Summary
Lipase from Candida cylindracea was immobilized by entrapment with photo-crosslinkable resin prepolymers or urethane prepolymers, and by covalent binding or by adsorption to different types of porous inorganic or organic supports. All of the immobilized lipase preparations thus obtained showed some activity for hydrolysis of olive oil. Lipase entrapped with a hydrophobic photo-cross-linkable resin prepolymer exhibited the highest activity, which was about 30% of that of the free counterpart. Entrapment method enabled lipase to gain operational stability. Semicontinuous hydrolysis of olive oil using immobilized lipase was also accomplished in a packed-bed reactor with a recycling system. In this reactor, immobilized lipase was observed to have the sufficient activity and stability.
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References
Bell G, Todd JR (1981) Hydrolysis of triglyceride by solid phase lipolytic enzymes of Rhizopus arrhizus in continuous reactor system. Biotechnol Bioeng 23:1703–1719
Brümmer W, Hennrich N, Klockow M, Lang H, Orth HD (1972) Preparation and properties of carrier-bound enzymes. Eur J Biochem 25:129–135
Fukui S, Sonomoto K, Itoh N, Tanaka A (1980) Several novel methods for immobilization of enzymes, microbial cells and organelles. Biochimie 62:381–386
Fukui S, Tanaka A, Iida T, Hasegawa E (1976) Application of photo-crosslinkable resin to immobilization of an enzyme. FEBS Lett 66:179–182
Kilara A, Shahani KM (1977) Preparation and properties of immobilized papain and lipase. Biotechnol Bioeng 19:1703–1714
Kosugi Y, Suzuki H (1973) Fixation of cell-bound lipase and properties of the fixed lipase as an immobilized enzyme. J Ferment Technol 51:895–903
Kubo H, Karube I, Suzuki S (1976) Electric field control of enzyme membrane activity. Biochem Biophys Res Commun 69:731–736
Lieberman RB, Ollis DF (1975) Hydrolysis of particulate tributyrin in a fluidized lipase reactor. Biotechnol Bioeng 17:1401–1419
Omata T, Iida T, Tanaka A, Fukui S (1979) Transformation of steroids by gel-entrapped Nocardia rhodocrous cells in organic solvent. Eur J Appl Microbiol Biotechnol 8:143–155
Omata T, Iwamoto N, Kimura T, Tanaka A, Fukui S (1981) Stereoselective hydrolysis of dl-menthyl succinate by gel-entrapped Rhodotorula minuta var. texensis cells in organic solvent. Eur J Appl Microbiol Biotechnol 11:199–204
Sonomoto K, Tanaka A, Omata T, Yamane T, Fukui S (1979) Application of photo-crosslinkable resin prepolymers to entrap microbial cells. Effects of increased cell-entrapping gel hydrophobicity on the hydrocortisone Δ 1-dehydrogenation. Eur J Appl Microbiol Biotechnol 6:325–334
Tanaka A, Yasuhara S, Gellf G, Osumi M, Fukui S (1978) Immobilization of yeast microbodies and the properties of immobilized microbody enzymes. Eur J Appl Microbiol Biotechnol 5:17–27
Tomizuka N, Ota Y, Yamada K (1966) Studies on lipase from Candida cylindracea. Part I. Purification and properties. Agric Biol Chem 30:576–584
Tosa T, Mori T, Chibata I (1969) Studies on continuous enzyme reactions. Part IV. Enzymatic properties of the DEAE-Sephadex-aminoacylase complex. Agric Biol Chem 33:1053–1059
Weetall HH (1976) Covalent coupling methods for inorganic support materials. Methods Enzymol 44:134–148
Yokozeki K, Yamanaka S, Takinami K, Hirose Y, Tanaka A, Sonomoto K, Fukui S (1982a) Application of immobilized lipase to regio-specific interesterification of triglyceride in organic solvent. Eur J Appl Microbiol Biotechnol 14:1–5
Yokozeki K, Yamanaka S, Utagawa T, Takinami K, Hirose Y, Tanaka A, Sonomoto K, Fukui S (1982b) Production of adenine arabinoside by gel-entrapped cells of Enterobacter aerogenes in water-organic cosolvent system. Eur J Appl Microbiol Biotechnol 14:225–231
Zaborsky OR, Ogletree J (1974) The immobilization of glucose oxidase via activation of its carbohydrate residues. Biochem Biophys Res Commun 61:210–216
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Kimura, Y., Tanaka, A., Sonomoto, K. et al. Application of immobilized lipase to hydrolysis of triacylglyceride. European J. Appl. Microbiol. Biotechnol. 17, 107–112 (1983). https://doi.org/10.1007/BF00499860
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DOI: https://doi.org/10.1007/BF00499860