Summary
The immunocytochemical localization of fatty acid binding protein (FABP) of liver type was studied at light and electron microscopic levels by the peroxidase-antiperoxidase (PAP) method using a specific polyclonal antibody against FABP in the liver of fed and fasted rats. In the liver of rats fed ad libitum, the intense immunoreactivity was confined to portions of the liver cell cytoplasm adjacent to the glycogen area. After 2-days' fasting, such a focal intracellular localization of the immunoreactivity was abolished, in association with the disappearance of the glycogen area, and was replaced by a diffuse distribution of the immunoreactivity throughout the cytoplasm, with higher intensity at the periphery of the cells. In liver cells exhibiting an overall hypertrophy of smooth endoplasmic reticulum (SER) induced by the treatment of fasted rats with phenobarbital, the peripheral localization of FABP immunoreactivity ramained unchanged compared with that obtained in the case of fasting alone, and the immunoreactivity did not occur in association with the proliferated SER in the central cytoplasm. These results suggest that FABP, although cytosolic in nature, changes its localization within the liver cells in response to the general metabolic alterations caused by the starvation, inferring that FABP is intimately involved in the intracellular transport and metabolism of free fatty acids.
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References
Bass NM, Mannings JA, Ockner RK (1985) Turnover and short term regulation of fatty acid binding protein in liver. J Biol Chem 260:9603–9607
Capron F, Coltoff-Schiller B, Johnson AB, Fleischner GM, Goldfischer S (1979) Immunocytochemical localization of hepatic ligandin and Z protein utilizing frozen sections for light and electron microscopy. J Histochem Cytochem 27:961–966
Cardell RR Jr (1971) Action of metabolic hormones on the fine structure of rat liver cells. I. Effects of fasting on the ultrastructure of hepatocytes. Am J Anat 131:21–54
De Man JCH, Blok APR (1966) Relationship between glycogen and agranular endoplasmic reticulum in rat hepatic cells. J Histochem Cytochem 14:135–146
Fulton AB (1982) How crowded is the cytoplasm? Cell 30:345–347
Gordon JI, Alpers DH, Ockner RK, Strauss AW (1983) The nucleotide sequence of rat liver fatty acid binding protein mRNA. J Biol Chem 258:3356–3363
Gordon JI, Elshourbagy N, Lowe JB, Liao W, Alpers DH, Taylor JM (1985) Tissue specific expression and developmental regulation of two genes coding for rat fatty acid binding proteins. J Biol Chem 260:1995–1998
Jones AL, Fawcett DW (1966) Hypertrophy of the agranular endoplasmic reticulum in hamster liver induced by phenobarbital (with a review on the functions of this organelle in liver). J Histochem Cytochem 14:215–232
Kondo H (1984) Reexamination of the reality or artifacts of the microtrabeculae. J Ultrastruct Res 87:124–135
Kugler JH (1967) Correlation of the glycogen concentration in rat liver and the appearance of glycogen and agranular endoplasmic reticulum. J R Microsc Soc 86:285–296
Masters BSS, Baron J, Taylor WE, Isaacson EL, Lo Spalluto J (1971) Immunochemical studies on electron transport chains involving cytochrome P-450. J Biol Chem 246:4143–4150
Mishkin S, Stein L, Gatmaitan Z, Arias IM (1972) The binding of fatty acids to cytoplasmic proteins: binding to Z protein in liver and other tissues of rat. Biochem Biophys Res Commun 47:997–1003
Newsholme EA, Start C (1973) Regulation in metabolism. J Wiley, London, pp 146–328
Ockner RK, Manning JA, Poppenhausen RB, Ho WKL (1972) A binding protein for fatty acids in cytosol of intestinal mucosa, myocardium, and other tissues. Science 177:56–58
Ockner RK, Manning JA, Kane JP (1982) Fatty acid binding protein: isolation from rat liver, characterization, and immunochemical quantification. J Biol Chem 257:7872–7878
Ouchterlony O (1958) Diffusion-in-gel methods for immunological analysis. Prog Allergy 5:1–78
Owen OE, Reichard GA Jr (1971) Fuels consumed by man: The interplay between carbohydrates and fatty acids. Prog Biochem Pharmacol 6:177–213
Porter KR, Byers HR, Ellisman MH (1979) The cytoskeleton. In: Schmidt FO, Worden FG (eds) The neuroscience IVth study program. MIT Press, Cambridge, Mass, pp 703–722
Reyes HA, Levi AJ, Gatmaitan Z, Arias IM (1971) Studies of Y and Z, two hepatic cytoplasmic organic anion-binding proteins: effect of drugs, chemicals, hormones, and cholestasis. J Clin Invest 50:2242–2252
Sacchettini JC, Said B, Schulz H, Gordon JI (1986) Rat heart fatty acid binding protein is highly homologous to the murine adipocyte 422 protein and the P2 protein of peripheral nerve myelin. J Biol Chem 261:8218–8223
Sternberger LA (1974) Immunocytochemistry. Prentice Hall, Englewood Cliffs, New Jersey
Takahashi K, Odani S, Ono T (1982) A close structural relationship of rat liver Z-protein to cellular retinoid binding proteins and peripheral nerve myelin P2 protein. Biochem Biophys Res Commun 106:1099–1105
Takahashi K, Odani S Ono T (1983) Isolation and characterization of the three fractions (DE-I, DE-II and DE-III) of rat-liver Z-protein and the complete primary structure of DE-II. Eur J Biochem 136:589–601
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Iseki, S., Kondo, H., Hitomi, M. et al. Immunocytochemical localization of hepatic fatty acid binding protein in the liver of fed and fasted rats. Histochemistry 89, 317–322 (1988). https://doi.org/10.1007/BF00500632
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DOI: https://doi.org/10.1007/BF00500632