Summary
We investigated the excretion of citric and isocitric acids in a strain of Saccharomycopsis lipolytica grown on either n-paraffins, glucose, or glycerol. These acids were excreted in the ratio of 67:33 on n-paraffins and roughly 92:8 on either glucose or glycerol. However, with all the carbon sources used, the relative amount of isocitric acid in the intracellular pool remained below 10%. The assimilation of citric and isocitric acids was prevented when glucose or glycerol were the carbon sources, but not when n-paraffins were used. Citric acid stopped isocitric acid assimilation. These phenomena of selective assimilation and/or uptake might explain the variations observed in the ratio of citric to isocitric acids excreted on different carbon sources.
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Tréton, B., Le Dall, M.T. & Heslot, H. Excretion of citric and isocitric acids by the yeast Saccharomycopsis lipolytica . European J. Appl. Microbiol. Biotechnol. 6, 67–77 (1978). https://doi.org/10.1007/BF00500857
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DOI: https://doi.org/10.1007/BF00500857