Summary
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1.
Jejunal loops of anaesthetized rats were perfused with hypo-, iso-, and hypertonic buffered solutions containing tritiated water. The blood flow, the disapperance rate (from the intestinal lumen), and the appearance rate (in the intestinal venous blood) were determined simultaneously.
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2.
A decrease of the blood flow from about 1.7 to 0.2 ml min−1g−1 wet tissue weight diminished the appearance and disappearance rate of tritiated water. An increase of the blood flow caused the reversed results. The appearance rate was almost completely determined by the blood flow indicating that the intestinal absorption of tritiated water is blood flow limited. Therefore the absorption rate of tritiated water characterizes badly the diffusive water permeability of the intestinal epithelium.
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3.
A water net flux directed towards the blood accelerated and towards the intestinal lumen retarded the intestinal absorption of tritiated water at low, intermediate, and high blood flow values.
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4.
The data were analysed by a modified four-compartment-model considering the variation of blood flow and water net flux. The water net flux influences the absorption of tritiated water during the penetration through the epithelium by solvent drag (sieving coefficient 1-σ=1.54±0.19) as well as by changing the blood flow rate through capillaries near the epithelium.
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References
Andersen, B., Ussing, H. H.: Solvent drag on non-electrolytes during osmotic flow through isolated toad skin and its response to antidiuretic hormone. Acta physiol. scand. 39, 228–239 (1957).
Andreoli, T. E., Troutman, S. L.: An analysis of unstirred layers in series with “tight” and “porous” lipid bilayer membranes. J. gen. Physiol. 57, 464–478 (1971).
Benson, J. A., Jr., Lee, P. R., Scholer, J. F., Kim, K. S., Bollman, J. L.: Water absorption from the intestine via portal and lymphatic pathways. Amer. J. Physiol. 184, 441–444 (1956).
Cope, F. W.: Nuclear magnetic resonance evidence using D2O for structured water in muscle and brain. Biophys. J. 9, 303–319 (1969).
Dainty, J.: Water relations of plant cells. Advanc. bot. Res. 1, 279–326 (1963).
— House, C. R.: “Unstirred layer” in frog skin. J. Physiol. (Lond.) 182, 66–78 (1966).
Fisher, R. B.: The absorption of water and of some small solute molecules from the isolated small intestine of the rat. J. Physiol. (Lond.) 130, 655–664 (1955).
Fordtran, J. S., Levitan, R., Bikerman, V., Burrows, B. A., Ingelfinger, F. J.: The kinetics of water absorption in the human intestine. Trans. Ass. Amer. Phycns 74, 195–206 (1961).
— Rector, F. C., Jr., Carter, N. W.: The mechanisms of sodium absorption in the human small intestine. J. clin. Invest. 47, 884–900 (1968).
——, Locklear, T. W., Ewton, M. F.: Water and solute movement in the small intestine of patients with sprue. J. clin. Invest. 46, 287–298 (1967).
Forth, W., Furukawa, E., Leopold, G., Rummel, W.: Vergleichende Untersuchungen über die Resorption 3H-markierter Herzglycoside. In: Radioisotope in Pharmakokinetik und klinischer Biochemie. W. Keiderling, G. Hoffmann u. H. Ladner. Stuttgart: Schattauer 1970.
—, Rummel, W.: Wirkung von Herzglykosiden auf Calcium-, Natrium-, Wasser-und Glukosetransport am isolierten Dünndarm. Helv. physiol. pharmacol. Acta 25, 8–23 (1967).
Grim, E., Lee, J. S., Visscher, M. B.: Water exchange between intestinal contents, tissues and blood. Amer. J. Physiol. 182, 359–363 (1955).
Hakim, A. A., Lifson, N.: Urea transport across intestinal mucosa in vitro. Amer. J. Physiol. 206, 1315–1320 (1964).
Hays, R. M.: A new proposal for the action of vasopressin, based on studies of a complex synthetic membrane. J. gen. Physiol. 51, 385–398 (1968).
—, Leaf, A.: The state of water in the isolated toad bladder in the presence and absence of vasopressin. J. gen. Physiol. 45, 933–948 (1962).
Herzer, R., Haberich, F. J.: Enterale Aufnahme und renale Ausscheidung von Tritium-markiertem Wasser bei wachen Ratten. Pflügers Arch. ges. Physiol. 292, 277–287 (1966).
Hindle, W., Code, C. F.: Some differences between duodenal and ileal sorption. Amer. J. Physiol. 203, 215–220 (1962).
Horowitz, S. B., Fenichel, I. R.: Diffusion and the transport of organic nonelectrolytes in cells. Ann. N. Y. Acad. Sci. 125, 572–594 (1965).
Hyden, S.: A turbidometric method for the determination of higher polyethylene glycols in biologic materials. K. Lantbr. Högsk. Ann. 22, 139–145 (1955).
Kedem, O., Katchalsky, A.: A physical interpretation of the phenomenological coefficients of membrane permeability. J. gen. Physiol. 45, 143–179 (1962).
——: Permeability of composite membranes. Trans. Faraday Soc. 59, 1918–1953 (1963).
Kidder III, G. W.: Unstirred layers in tissue respiration; application to studies of frog gastric mucosa. Amer. J. Physiol. 219, 1789–1795 (1970).
Lee, P. R., Code, C. F., Scholer, J. F.: The influence of varying concentrations of sodium chloride on the rate of absorption of water from the stomach and small bowel of human beings. Gastroenterology 29, 1008–1015 (1955).
Lembeck, F., Sewing, K.-Fr., Winne, D.: Der Einfluß von 5-Hydroxytryptamin auf die Resorption von Tritium-Wasser (HTO) aus dem Dünndarm der Ratte. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 247, 100–109 (1964).
Levitt, D. G., Hakim, A. A., Lifson, N.: Evaluation of components of transport of sugars by dog jejunum in vivo. Amer. J. Physiol. 217, 777–783 (1969).
Lifson, N., Gruman, L. M., Levitt, D. G.: Diffusive-convective models for intestinal absorption of D2O. Amer. J. Physiol. 215, 444–454 (1968).
—, Hakim, A. A.: Simple diffusive-convective model for intestinal absorption of a nonelectrolyte (urea). Amer. J. Physiol. 211, 1137–1146 (1966).
Lindeman, B., Solomon, A. K.: Permeability of luminal surface of intestinal mucosal cells. J. gen. Physiol. 45, 801–810 (1962).
Noyan, A.: Water absorption from the intestine via portal and lymphatic pathway in rats. Proc. Soc. exp. Biol. (N. Y.) 117, 317–320 (1964).
Ochsenfahrt, H.: The mucosal-serosal transfer of drugs in the rat jejunum with and without blood flow. Naunyn-Schmiedebergs Arch. Pharmak. 270, Suppl. R 102 (1971).
— Winne, D.: Der Einfluß der Durchblutung auf die Resorption von Arzneimitteln aus dem Jejunum der Ratte. Naunyn-Schmiedebergs Arch. Pharmak. 264, 55–75 (1969).
Ochsenfahrt, H., Winne, D.: Der Einfluß des Wassernettofluxes auf die Resorption von Arzneimitteln. Naunyn-Schmiedebergs Arch. Pharmak. 266, 414–415 (1970).
Sewing, K.-Fr.:, Winne, D., Lembeck, F. Der Einfluß von 5-Hydroxytryptamin auf die Resorption von Tritium-Wasser (HTO) aus dem Colon der Ratte in vivo. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 252, 286–290 (1965).
Shields, R.: The absorption and secretion of fluid and electrolytes by the obstructed bowel. Brit. J. Surg. 52, 774–779 (1965).
— Code, C. F.: Effect of increased portal pressure on sorption of water and sodium from the ileum of dogs. Amer. J. Physiol. 200, 775–780 (1961).
Smyth, D. H., Wright, E. M.: Streaming potentials in the rat small intestine. J. Physiol. (Lond.) 182, 591–602 (1966).
Soergel, K. H., Whalen, G. E., Harris, J. A.: Passive movement of water and sodium across the human small intestinal mucosa. J. appl. Physiol. 24, 40–48 (1968).
Staverman, A. J.: The theory of measurement of osmotic pressure. Rec. Trav. chim. Pays-Bas 70, 344–352 (1951).
Ther, L., Winne, D.: Drug absorption. Ann. Rev. Pharmacol. 11, 57–70 (1971).
Visscher, M. B., Fetcher, E. S., Jr., Carr, C. W., Gregor, H. P., Bushey, M. S., Barker, D. E.: Isotopic tracer studies on the movement of water and ions between intestinal lumen and blood. Amer. J. Physiol. 142, 550–575 (1944).
Wang, J. H., Robinson, C. V., Edelman, I. S.: Self-diffusion and structure of liquid water. III. Measurement of the self-diffusion of liquid water with 2H, 3H and 18O as tracers. J. Amer. chem. Soc. 75, 466–470 (1953).
Winne, D.: Der Einfluß einiger Pharmaka auf die Darmdurchblutung und die Resorption tritiummarkierten Wassers aus dem Dünndarm der Ratte. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 254, 199–224 (1966).
—: Formal kinetics of water and solute absorption with regard to intestinal blood flow. J. theor. Biol. 27, 1–18 (1970a).
—: Der Einfluß der Durchblutung auf die Wasser-und Salzresorption im Jejunum der Ratte. Naunyn-Schmiedebergs Arch. Pharmak. 265, 425–441 (1970b).
—: Die Pharmakokinetik der Resorption bei Perfusion einer Darmschlinge mit variabler Durchblutung. Naunyn-Schmiedebergs Arch. Pharmak. 268, 417–433 (1971a).
—: Die Bedeutung der Blutdränage in der Pharmakokinetik der enteralen Resorption. Med. Welt (Stuttg.) 22, 632–640 (1971b).
— Ochsenfahrt, H.: Die formale Kinetik der Resorption unter Berücksichtigung der Darmdurchblutung. J. theor. Biol. 14, 293–315 (1967).
— Remischovsky, J.: Intestinal blood flow and absorption of non-dissociable substances. J. Pharm. Pharmacol. 22, 640–641 (1970).
——: Der Einfluß der Durchblutung auf die Resorption von Harnstoff, Methanol und Äthanol aus dem Jejunum der Ratte. Naunyn-Schmiedebergs Arch. Pharmak. 268, 392–416 (1971a).
——: Der Einfluß der Durchblutung auf die Resorption von Polyalkoholen aus dem Jejunum der Ratte. Naunyn-Schmiedebergs Arch. Pharmak. 270, 22–40 (1971b).
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Winne, D. The influence of blood flow and water net flux on the absorption of tritiated water from the jejunum of the rat. Naunyn-Schmiedeberg's Arch. Pharmacol. 272, 417–436 (1972). https://doi.org/10.1007/BF00501248
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DOI: https://doi.org/10.1007/BF00501248