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Physiological requirements for degradation of lignin and lignin-related substances by Sporotrichum pulverulentum

  • Applied Microbiology
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European journal of applied microbiology and biotechnology Aims and scope Submit manuscript

Summary

Vanillic and syringic acid metabolism in the white-rot fungus Sporotrichum pulverulentum Nov. at varied concentrations of glucose and nitrogen was investigated. Vanillic acid metabolism was stimulated by 20.6 mM nitrogen and repressed by 1% glucose when measured as formation of 14CO2 from 14C-labelled vanillic acids. Metabolism of 14C-methoxy labelled syringic acid was initially most rapid in the presence of 0.25% glucose and 20.6 mM nitrogen but total 14CO2 evolution was higher in cultures containing 1% glucose.

The metabolism of veratrylglycerol-β-guaiacyl ether by S. pulverulentum was repressed by high nitrogen in the medium in the same way as lignin degradation. Conversion of labelled spruce and poplar lignins to 14CO2 by S. pulverulentum was highest in the presence of 1% glucose and 2.6 mM nitrogen, while 0.1% yeast extract had a repressive effect.

The results indicate that monomers are degraded during both primary and secondary metabolism of the fungus, whereas dimers and more complex lignin structures are attacked during the secondary phase. The switch in metabolism of lignin degradation products seems to occur when these products have a structure between monomeric and dimeric forms.

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Authors and Affiliations

  1. Swedish Forest Products Research Laboratory, Box 5604, S-114 86, Stockholm, Sweden

    Paul Ander, Karl-Erik Eriksson & Hui-sheng Yu

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  1. Paul Ander
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  2. Karl-Erik Eriksson
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  3. Hui-sheng Yu
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Ander, P., Eriksson, KE. & Yu, Hs. Physiological requirements for degradation of lignin and lignin-related substances by Sporotrichum pulverulentum . European J. Appl. Microbiol. Biotechnol. 18, 374–380 (1983). https://doi.org/10.1007/BF00504748

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