Summary
An assay for the determination of the protein content of whole cells immobilized in cross-linked synthetic gels was developed. The assay is based on a three step procedure: a) methanol dehydration, b) protein extraction by 1.0 M alkali at 125°C c) colorimetric assay of the extracted protein according to Bradford's procedure (Bradford M. M. (1976), Anal. Biochem. 72:248–254). The procedure worked out was found adequate for the determination of the protein content of microbial cells immobilized in synthetic and native polymer-gel-systems.
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References
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Freeman, A., Blank, T. & Aharonowitz, Y. Protein determination of cells immobilized in cross-linked synthetic gels. European J. Appl. Microbiol. Biotechnol. 14, 13–15 (1982). https://doi.org/10.1007/BF00507997
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DOI: https://doi.org/10.1007/BF00507997