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Immobilization of thylakoids in porous particles and stabilization of the photochemical processes by glutaraldehyde action at subzero temperature

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Summary

Lettuce thylakoids were immobilized by the action of glutaraldehyde at sub-zero temperature in presence of albumin. Particles with sponge structure and good mechanical properties were obtained. The activity yield after immobilization was found equal to 70% for the oxygen production. Photosystems I and II were both active after immobilization. The yield for the ATP production was 27%. An increase of stability of the immobilized thylakoids was observed when stored under illumination.

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Authors and Affiliations

  1. Laboratoire de Technologie Enzymatique, E.R.A. no 338 du C.N.R.S., B.P. 233 U.T.C., F-60206, Compiègne, France

    M. F. Cocquempot & D. Thomas

  2. Laboratoire de Physiologie Cellulaire Végétale, L.A. no du C.N.R.S., Université Paris-Sud, F-91405, Orsay, France

    M. L. Champigny & A. Moyse

Authors
  1. M. F. Cocquempot
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  2. D. Thomas
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  3. M. L. Champigny
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  4. A. Moyse
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Cocquempot, M.F., Thomas, D., Champigny, M.L. et al. Immobilization of thylakoids in porous particles and stabilization of the photochemical processes by glutaraldehyde action at subzero temperature. European J. Appl. Microbiol. Biotechnol. 8, 37–41 (1979). https://doi.org/10.1007/BF00510264

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  • DOI: https://doi.org/10.1007/BF00510264

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