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Potentiation by deprenyl of the autoreceptor-mediated inhibition of [3H]-5-hydroxytryptamine release by 5-methoxytryptamine

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Summary

The 5-hydroxytryptamine (5HT) receptor agonist, 5-methoxytryptamine, inhibited in a concentration-dependent manner the electrically-evoked release of 3H-5HT from superfused rat hypothalamic slices, with an IC50 of 560 nmol/l, without affecting the spontaneous outflow of radioactivity. In the presence of the selective monoamine oxidase B (MAO B) inhibitor, (−)-deprenyl (1 μmol/l), the concentration-effect curve for 5-methoxytryptamine was shifted significantly to the left, and the IC50 was decreased to 25 nmol/l. Under the same experimental conditions, the potency of the 5HT receptor agonist lysergic acid diethylamide (LSD) at inhibiting the electrically-evoked release of 3H-5HT was the same in the presence as well as in the absence of (−)-deprenyl. The IC50 values for LSD were 34 nmol/l in the absence of deprenyl, and 31 nmol/l in the presence of the MAO B inhibitor. It is concluded that deprenyl potentiates the inhibition by 5-methoxytryptamine of 3H-5HT release, by preventing its inactivation through MAO B. Since 5-methoxytryptamine may be present in the pineal gland of some species, the potent effects of this 5-HT receptor agonist on seretoninergic neutrotransmission may be of physiological relevance.

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Authors and Affiliations

  1. Department of Biology, Laboratoires d'Etudes et de Recherches Synthélabo (L.E.R.S.) 58, rue de la Glacière, F-75013, Paris, France

    Anne-Marie Galzin & Salomon Z. Langer

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  1. Anne-Marie Galzin
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  2. Salomon Z. Langer
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Galzin, AM., Langer, S.Z. Potentiation by deprenyl of the autoreceptor-mediated inhibition of [3H]-5-hydroxytryptamine release by 5-methoxytryptamine. Naunyn-Schmiedeberg's Arch. Pharmacol. 333, 330–333 (1986). https://doi.org/10.1007/BF00512949

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  • DOI: https://doi.org/10.1007/BF00512949

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