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Layered structure of trichoderma cell walls

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Abstract

Trichoderma pseudokoningii mycelium was treated sequentially with the following enzymes; (1) lysing enzyme and laminarinase, (2) pronase, and (3) chitinase. (1) Removed the outermost amorphous glucan layer and revealed a striated layer which was removed by (2). Microfibrils were exposed and removed by (3). The wall thus has a layered structure comprising glucan, protein and/or glycoprotein, and chitin.

Glucan was prepared and subjected to methylation and hydrolysis. 1,3,5,6-Tetra-O-acetyl-2,4-di-O-methyl-D-glucose was identified suggesting that the 1–3 and 1–6 glucans are cross-linked at intervals.

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Author notes

  1. Derek Boothby

    Present address: Department of Food and Biological Sciences, Polytechnic of North London, N7 8DB, Holloway, London, UK

Authors and Affiliations

  1. School of Chemistry, Polytechnic of North London, N7 8DB, Holloway, London, UK

    Mohamed S. Jennah & Bruce E. Davison

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  1. Mohamed S. Jennah
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  2. Bruce E. Davison
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  3. Derek Boothby
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Jennah, M.S., Davison, B.E. & Boothby, D. Layered structure of trichoderma cell walls. Arch. Microbiol. 133, 330–331 (1982). https://doi.org/10.1007/BF00521300

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  • DOI: https://doi.org/10.1007/BF00521300

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