Abstract
In vitro protoscolices of Echinococcus granulosus grew in a vesicular direction. After 9 days the laminated layer was observed; it reached thicknesses up to 140 Μm. By day 125 of culture, the cysts had reached sizes of up to 4.875 Μm and a high percentage of cysts had been obtained (10.4%). The ultrastructure of these cysts was studied and the existence of a germinal layer was demonstrated.
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Rodriguez-Caabeiro, F., Casado, N. Evidence of in vitro germinal layer development in Echinococcus granulosus cysts. Parasitol Res 74, 558–562 (1988). https://doi.org/10.1007/BF00531634
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DOI: https://doi.org/10.1007/BF00531634