Skip to main content
SpringerLink
Log in

The La antigen shuttles between the nucleus and the cytoplasm in CV-1 cells

  • Original Articles
  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

Recently we established a monoclonal antibody against the La-protein (Bachmannet al., Proc. Natl. Acad. Sci. USA, 83, 7770, 1986). The antibody gives a nuclear speckled type staining and, in addition, a perinuclear cytoplasmic staining on cultured cells in immunofluorescence microscopy. After inhibition of RNA synthesis the La-protein is transported into the cytoplasm. After prolonged inhibition it returns into the nucleus forming large growing speckles. The transport into the nucleus apparently depends on glycosylation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

FITC:

Fluorescein Isothiocyanate

RITC:

Rhodamine Isothiocyanate

scRNP:

small cytoplasmic Ribonucleoprotein

snRNP:

Small nuclear Ribonucleoprotein

mab:

Monoclonal antibody

Ig:

Immunoglobulin

BSA:

Bovine Serum Albumin

PBS:

Phosphate Buffered Saline

PMSF:

Phenylmethanesulfonyl Fluoride

SDS:

Sodium Dodecyl Sulfate

EGTA:

Ethyleneglycol bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic Acid

MCTD:

Mixed Connective Tissue Disease

SLE:

Systemic Lupus Erythematosus

References

  1. Zasloff M: tRNA transport from the nucleus in a eukaryotic cell: carrier mediated translocation process. Proc Natl Acad Sci USA 80:6436–6440, 1983

    Google Scholar 

  2. Schröder HC, Bachmann M, Diehl-Seifert B, Müller WEG: Transport of mRNA from nucleus to cytoplasm. Progr. Nucleic Acid Res Mol Biol 34:89–142, 1987

    Google Scholar 

  3. Schröder HC, Rottmann M, Bachmann M, Diehl-Seifert B, Müller WEG: Purification and characterization of the major nucleoside triphosphatase from rat liver nuclear envelopes. J Biol Chem 261:663–668, 1987

    Google Scholar 

  4. Mattaj IW, Lienhard S, Zeller R, DeRobertis EM: Nuclear exclusion of transcription factor IIIA and the 42S particle transfer RNA-binding protein in Xenopus oocytes: A possible mechanism for gene control? J Cell Biol 97:1261–1265, 1983

    Google Scholar 

  5. Mattaj IW: Cap-trimethylation of U snRNA is cytoplasmic and dependent on U snRNA protein binding. Cell 46:905–911, 1986

    Google Scholar 

  6. Habets WJ, Berden JHM, Hoch SO, Van Venrooij WJ: Further characterization and subcellular localization of Sm and U1 ribonucleoprotein antigens. Eur J Immunol 15:992–997, 1985

    Google Scholar 

  7. Lerner M, Steitz JA: Snurps and Scyrps. Cell 25:298–300, 1981

    Google Scholar 

  8. Brunet C, Sri-Widada J, Jeanteur P: SnRNP's and scRNP's in Eukaryotic Cells. In: FE Hahn, DJ Kopecko and WEG Müller (eds) Progr Mol Subcell Biol. Springer Verlag Berlin, Heidelberg, New York, Tokyo, 1985, pp 1–52

    Google Scholar 

  9. Hendrick JP, Wolin S, Rinke J, Lerner M, Steitz JA: Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: Further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells. Mol Cell Biol 12:1138–1149, 1981

    Google Scholar 

  10. Rinke J, Steitz JA: Precursor molecules of both human 5S ribosomal RNA and transfer RNAs are bound by a cellular protein reactive with anti-La Lupus antibodies. Cell 29:149–159, 1982

    Google Scholar 

  11. Chambers JC, Kurilla MG, Keen JD: Association between the 7S RNA and the Lupus La protein varies among cell types. J Biol Chem 258:11438–11441, 1983

    Google Scholar 

  12. Madore SJ, Wieben ED, Pederson T: Eukaryotic small ribonucleoproteins. J Biol Chem 259:1929–1933, 1984

    Google Scholar 

  13. Bachmann M, Mayet WJ, Schröder HC, Pfeifer K, Meyer zum Büschenfelde K-H, Müller WEG: Association of La and Ro antigens with intracellular structures in HEp-2 carcinoma cells. Proc Natl Acad Sci USA 83:7770–7774, 1986

    Google Scholar 

  14. Bachmann M, Trautmann F, Messer R, Zahn RK, Meyer zum Büschenfelde K-H, Müller WEG: Association of a polyuridylate-specific endoribonuclease with small nuclear ribonucleoproteins which had been isolated by affinity chromatography using antibodies from a patient with systemic Inpus erythematosus. Eur J Biochem 136:447–451, 1983

    Google Scholar 

  15. Kurata N, Tan EM: Identification of antibodies to nuclear acidic antigens by counterimmunoelectrophoresis. Arth Rheum 19:574–580, 1976

    Google Scholar 

  16. Gramzow M, Bachmann M, Uhlenbruck G, Dorn A, Müller WEG: Identification and further characterization of the specific cell binding fragment from sponge aggregation factor. J Cell Biol 102:1344–1349, 1986

    Google Scholar 

  17. Bachmann M, Schröder HC, Wagner KG, Mayet WJ, Pfeifer K, Müller WEG: Purification and characterization of the Ro and La antigens. Modulation of their binding affinity to poly(U) by phosphorylation and the presence of ATP. Biol Chem Hoppe-Seyler 367:671–680, 1986

    Google Scholar 

  18. Bachmann M, Mayet WJ, Schröder HC, Pfeifer K, Meyer zum Büschenfelde K-H, Müller WEG: Identification of the Ro and La antigen in the endoribonuclease VII RNP complex. Biochemical J 243:189–194, 1987

    Google Scholar 

  19. Blobel G, Potter R: Nuclei from rat liver: Isolation method that combines purity with high yield. Science 154:1662–1665, 1966

    Google Scholar 

  20. Bachmann M, Messer R, Trautmann F, Müller WEG: 12S small nuclear ribonucleoprotein-associated acidic and pyrimidine-specific endoribonuclease from calf thymus and L5178y cells. Biochim Biophys Acta 783:89–99, 1984

    Google Scholar 

  21. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685, 1970

    Google Scholar 

  22. Towbin H, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354, 1979

    Google Scholar 

  23. McNeilage LJ, Wittingham S: Use of the Bio-Rad silver stain to identify gel purified RNA components of small nuclear ribonucleoproteins. J Immunol Methods 66:253–260, 1984

    Google Scholar 

  24. Müller WEG, Mayet WJ, Bachmann M, Meyer zum Büschenfelde K-H, Pfeifer K, Diehl-Seifert B, Schröder HC: Agecorrelated changes in the lupus erythematosus antigens Ro, La, Sm and RNP of the thymus gland. Compr Gerontol A 1:40–44, 1987

    Google Scholar 

  25. Murphy JT, Burgess RR, Dahlberg JE, Lund E: Transcription of a gene for human U1 small nuclear RNA. Cell 29:265–274, 1982

    Google Scholar 

  26. Reichel R, Benecke B-J: Reinitiation of synthesis of small cytoplasmic RNA species K and L in isolated HeLa cell nucleiin vitro. Nucl Acids Res 8:225–234, 1980

    Google Scholar 

  27. Elbein AD: The tunicamycins-useful tools for studies on glycoproteins. TIBS 6:219–221, 1981

    Google Scholar 

  28. Stefano JE: Purified Lupus antigen La recognizes an oligouridylate stretch common to the 3′-termini of RNA polymerase III transcripts. Cell 36:145–154, 1984

    Google Scholar 

  29. Francoeur AM, Mathews MB: Interaction between VA RNA and the Lupus antigen La: Formation of a ribonucleoprotein particlein vitro. Proc Natl Acad Sci USA 79:6772–6776, 1982

    Google Scholar 

  30. Nyman U, Hallman H, Hadlaczky G, Petterson I, Sharp G, Ringertz NR: Intranuclear localization of snRNP antigens. J Cell Biol 102:127–145, 1986

    Google Scholar 

  31. Rosa MD, Gottlieb E, Lerner MR, Steitz JA: Striking similarities are exhibited by two small Epstein-Barr virus-encoded ribonucleic acids and the adenovirus-associated ribonucleic acids VAI and VAII. Mol Cell Biol 1:785–796, 1981

    Google Scholar 

  32. Hamelin R, Chan EK, Tan EM: Antibodies against small nuclear ribonucleoproteins immunoprecipitate complexes containing ts110 Moloney murine sarcoma virus genomic and messenger RNAs. Virology 152:87–99, 1986

    Google Scholar 

  33. Gottlieb E, Steitz J: The mammalian La protein is an RNA polymerase III transcription termination factor. Mol Biol Rep 12:243, 1987

    Google Scholar 

  34. Jelinek W, Leinwand L: Low molecular weight RNAs hydrogen bonded to nuclear and cytoplasmic poly(A)-terminated RNA from cultured chinese hamster ovary cells. Cell 156:205–214, 1978

    Google Scholar 

  35. Harada F, Kato N, Hoshino H: Series of 4.5S RNAs associated with poly(A)-containing RNAs of rodent cells. Nucleic Acids Res 7:909–917, 1979

    Google Scholar 

  36. Harada F, Kato N: Nucleotide sequences of 4.5S RNAs associated with poly(A)-containing RNAs of mouse and hamster cells. Nucleic acids Res 8:1273–1285, 1980

    Google Scholar 

  37. Kato N, Hoshino HO, Harada F: Nucleotide sequence of 4.5S RNA (C8 or hY5) from HeLa cells. Biochem Biophys Res Commun 99:1477–1485, 1982

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Physiologische Chemie, Universität, Duesbergweg 6, 6500, Mainz, Germany

    Michael Bachmann, Karin Pfeifer, Heinz C. Schröder & Werner E. G. Müller

Authors
  1. Michael Bachmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karin Pfeifer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Heinz C. Schröder
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Werner E. G. Müller
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bachmann, M., Pfeifer, K., Schröder, H.C. et al. The La antigen shuttles between the nucleus and the cytoplasm in CV-1 cells. Mol Cell Biochem 85, 103–114 (1989). https://doi.org/10.1007/BF00577106

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00577106

Key words

Search

Navigation