Skip to main content
SpringerLink
Log in

Effects of prostaglandins and other putative chemical intermediaries on the activity of canine testicular polymodal receptors studied in vitro

  • Excitable Tissues and Central Nervous Physiology
  • Published:
Pflügers Archiv Aims and scope Submit manuscript

Abstract

(1) An in vitro testis-superior spermatic nerve preparation was used to evaluate the effects of chemical agents applied in the bathing solution. Both directly evoked discharges and responses to algesic solutions [bradykinin (BK) 9×10−8 M, hypertonic saline 616 mM and high K+ solution 60 mM] of polymodal receptors were studied. (2) Prostaglandin (PG)-E2 (1.4×10−6–1.4×10−5 M) and serotonin (5-HT) (1.1×10−6 to 1.4×10−4 M) had only a weak excitatory effect. However, test responses to algesic substances were regularly greatly increased by PG-E2,-I2 and 5-HT. Concentrations of PG-E2 of 1.4×10−8 M or grealer augmented BK responses; higher concentrations and/or longer applications were needed to enhance responses to algesic salt solutions. Effective concentrations for the PGs and 5-HT were near those reported for inflamed tissues and exudate. (3) Aspirin (ASA) (5.5×10−4 M or greater, for more than 4 min) suppressed the responses to BK but not those evoked by hypertonic saline. The ASA effect on the BK response was largely restored by an addition of PG-E2. (4) Substance P also had a weak excitatory effect on some polymodal receptors, but no significant enhancement of the response to BK was noted. (5) These results further support a role of polymodal receptors in transmitting nociceptive information, of inflammatory origin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Armstrong D, Dry RML, Keele CA, Markham JW (1953) Observations on chemical excitants of cutaneous pain in man. J Physiol 120:326–351

    Google Scholar 

  2. Barbieri EJ, Orzechowski RF, Rossi GV (1977) Measurement of prostaglandin E2 in an inflammatory exudate: effects of nonsteroidal anti-inflammatory agents. J Pharmacol Exp Ther 201:769–777

    Google Scholar 

  3. Beck PW, Handwerker HO (1974) Bradykinin and serotonin effects on various types of cutaneous nerve fibres. Pflügers Arch 347:209–222

    Google Scholar 

  4. Chahl LA, Iggo A (1977) The effects of bradykinin and prostaglandin E1 on rat cutaneous afferent nerve activity. Br J Pharmacol 59:343–347

    Google Scholar 

  5. Collier HOJ, Schneider C (1969) Profiles of activity in rodents of some narcotic and narcotic antagonist drugs. Nature 224:610–611

    Google Scholar 

  6. Erspamer V (1966) Occurrence of indolealkylamines in nature. In: Erspamer V (ed) Handbuch der Experimentellen Pharmakologie, vol 19. Springer, Berlin Heidelberg New York, pp 132–181

    Google Scholar 

  7. Essman WB (1978) Serotonin distribution in tissues and fluids. In: Essman WB (ed) Availability, localization and disposition, vol 1 Spectrum, New York, pp 15–179

    Google Scholar 

  8. Ferreira SH, Moncada S, Vane JR (1971) Indomethacin and aspirin abolish prostaglandin release from the spleen. Nature (New Biol) 231:237–239

    Google Scholar 

  9. Ferreira SH, nakamura M, Castro MSdeA (1978) The hyperalgesic effects of prostacyclin and prostaglandin E2. Prostaglandins 16:31–37

    Google Scholar 

  10. Fitzgerald M, Lynn B (1979) The weak excitation of some cutaneous receptors in cats and rabbits by synthetic substance P. J Physiol 293:66–67

    Google Scholar 

  11. Flower RJ, Moncada S, Vane JR (1980) Analgesic-antipyretics and anti-inflammatory agents; drugs employed in the treatment of gout. In: Gilman AG, Goodman LS, Gilman A (eds) Goodman and Gilman's: the pharmacological basis of therapeutics. Macmillan. New York, pp 682–728

    Google Scholar 

  12. Fock S, Mense S (1976) Excitatory effects of 5-hydroxytryptamine, histamine and potassium ions on muscular group IV afferent units: a comparison with bradykinin. Brain Res 105:459–469

    Google Scholar 

  13. Greaves MW, Sondergaard J, McDonald-Gibson W (1971) Recovery of prostaglandins in human cutaneous inflammation. Br Med J 2:258–260

    Google Scholar 

  14. Handwerker HO (1976) Pharmacological modulation of the discharge of nociceptive C-fibres. In: Zotterman Y (ed) Sensory functions of the skin in primate. Pergamon, Oxford, pp 427–439

    Google Scholar 

  15. Higgs GA, Moncada S, Salmon JA, Seager K (1983) The source of thromboxane and prostaglandins in experimental inflammation. Br J Pharmacol 79:863–868

    Google Scholar 

  16. Hökfelt T, Kellerth J-O, Nilsson G, Pernow B (1975) Experimental immunohistochemical studies on the localization and distribution of substance P in cat primary sensory neurons. Brain Res 100:235–252

    Google Scholar 

  17. Horton EW (1963) Action of prostaglandin E1 on tissues which respond bradykinin. Nature 200:892–893

    Google Scholar 

  18. Johansson S-A (1960) 5-Hydroxytryptamine in burns. Acta Physiol Scand 48:126–132

    Google Scholar 

  19. Johnson AR, Erdoes EG (1973) Release of histamine from mast cells by vasoactive peptides. Proc Soc Exp Biol Med 142:1252–1256

    Google Scholar 

  20. Juan H (1979) The pain enhancing effect of PG-I2. Agents Action 9:204–212

    Google Scholar 

  21. Juan H, Sametz W, Petronijevic S, Lembeck F (1984) Prostaglandin release and nociceptor stimulation by peptides. Naunyn-Schmiedeberg's Arch Pharmacol 326:64–68

    Google Scholar 

  22. Katori M, Harada Y, Tanaka K, Miyazaki H, Ishibashi M, Yamashita Y (1980) Changes of prostaglandin and thromboxane levels in pleural fluid of rat carrageenin-induced pleurisy. In: Samuelsson B, Ramwell W Paoletti R (eds) Advances in prostaglandin and thromboxane research, vol 8. Raven Press, New York, pp 1733–1737

    Google Scholar 

  23. Kumazawa T, Mizumura K (1979) Effects of synthetic substance P on unit discharges of testicular nociceptors of dogs. Brain res 170:553–557

    Google Scholar 

  24. Kumazawa T, Mizumura K (1980) Chemical responses of polymodal receptors of the scrotal content in dogs. J Physiol 299:219–231

    Google Scholar 

  25. Kumazawa T, Mizumura K (1980) Mechanical and thermal responses of polymodal receptors recorded from the superior spermatic nerve of dogs. J Physiol 299:233–245

    Google Scholar 

  26. Kumazawa T, Mizumura K (1983) Temperature dependency of the chemical responses of the polymodal receptor units in vitro. Brain Res 278:305–307

    Google Scholar 

  27. Kumazawa T, Mizumura K (1984) Abnormal activity of polymodal receptors induced by clioquinol (5-chloro-7-iodo-8-hydroxyquinoline). Brain Res 310:185–188

    Google Scholar 

  28. Kumazawa T, Mizumura K, Sato J (1987) Response properties of polymodal receptors studied using in vitro testis-superior spermatic nerve preparations of dogs. J Neurophysiol 57 (in press)

  29. Lembeck F, Holzer P (1979) Substance P as neurogenic mediator of antidromic vasodilation and neurogenic plasma extravasation. Naunyn-Schmiedeberg's Arch Pharmacol 310: 175–184

    Google Scholar 

  30. Lembeck F, Juan H (1974) Interaction of prostaglandins and indomethacin with algesic substances. Naunyn-Schmiedeberg's Arch Pharmacol 285:301–313

    Google Scholar 

  31. Lembeck F, Popper H, Juan H (1976) Release of prostaglandins by bradykinin as an intrinsic mechanism of its algesic effect. Naunyn-Schmiedeberg's Arch Pharmacol 294:69–73

    Google Scholar 

  32. Lim RKS, Guzman F, Rodgers DW, Goto K, Braun C, Dickenson GD, Engle RJ (1964) Site of action of narcotic and non-narcotic analgesics determined by blocking bradykinin-evoked visceral pain. Arch Int Pharmacodyn Thes 152:25–58

    Google Scholar 

  33. Mense S (1981) Sensitization of group IV muscle receptors to bradykinin by 5-hydroxytryptamine and prostaglandin-E2. Brain Res 225:95–105

    Google Scholar 

  34. Mense S (1982) Reduction of the bradykinin-induced activation of feline group III and IV muscle receptors by acetylsalicylic acid. J Physiol 326:269–283

    Google Scholar 

  35. Moncada S, Ferreira SH, Vane JR (1975) Inhibition of prostaglandin biosynthesis as the mechanism of analgesia of aspirinlike drugs in the dog knee joint. Eur J Pharmacol 31:250–260

    Google Scholar 

  36. Mullane KM, Moncada S (1980) Prostacyclin release and the modulation of some vasoactive hormones. Prostaglandins 20:25–49

    Google Scholar 

  37. Ohuchi K, Sato H, Tsurufuji S (1976) The content of prostaglandin E and prostaglandin F2 in the exudate of carrageenin granuloma of rats. Biochim Biophys Acta 424:439–448

    Google Scholar 

  38. Olgart L, Gazerius B, Brodin E, Nilsson G (1977) Release of substance P-like immunoreactivity from the dental pulp. Acta Physiol Scand 101:510–512

    Google Scholar 

  39. Rosell S, Olgart L, Gazelius B, Panopoulos P, Folkers K, Horig J (1981) Inhibition of antidromic and substance P-induced vasodilatation by a substance P antagonist. Acta Physiol Scand 111:381–382

    Google Scholar 

  40. Schaible HG, Schmidt RF (1985) Coding of pain information from joint. In: Rowe MH, Willis WD (ed) Development, organization, and processing in somatosensory pathways. Liss, New York, pp 309–316

    Google Scholar 

  41. Sicuteri F (1968) Sensitization of nociceptors by 5-hydroxytryptamine in man. In: Lim RKS, Armstrong D, Pardo EG (eds) Pharmacology of pain. Pergamon, New York, pp 57–86

    Google Scholar 

  42. Terashima R, Anderson FL, Jubiz W (1976) Prostaglandin E release in the dog: effect of sodium. Am J Physiol 231:1429–1432

    Google Scholar 

  43. Vane JR (1971) Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nature (New Biol) 231:232–235

    Google Scholar 

  44. Willis AL (1969) Release of histamine, kinin and prostaglandins during carrageenin-induced inflammation in the rat. In: Montegazza P, Horton EW (eds) Prostaglandins, peptides, and amines. Academic Press, London, pp 31–38

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Nervous and Sensory Functions, The Research Institute of Environmental Medicine, Nagoya University, Furo-cho, Chikusa-ku, 464, Nagoya, Japan

    K. Mizumura, J. Sato & T. Kumazawa

Authors
  1. K. Mizumura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Sato
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. Kumazawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mizumura, K., Sato, J. & Kumazawa, T. Effects of prostaglandins and other putative chemical intermediaries on the activity of canine testicular polymodal receptors studied in vitro. Pflugers Arch. 408, 565–572 (1987). https://doi.org/10.1007/BF00581157

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00581157

Key words

Search

Navigation