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Effects of halothane on caffeine-induced tension transients in functionally skinned myocardial fibers

  • Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology
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Abstract

The effects of halothane on caffeine-induced tension transients in functionally skinned myocardial fibers were investigated. Fiber bundles from mechanically disrupted rabbit right ventricular papillary muscles were mounted on a tension transducer. The fiber preparation was loaded with Ca2+; Ca2+ was then released by the use of caffeine (25 mM); and the area of the resulting tension transient was measured. Each preparation was sequentially transferred from control to test to control solution. The control solutions were equilibrated with 100% N2, and the test solutions with a mixture of N2 and various halothane concentrations. The preparation was exposed to halothane during the Ca2+ uptake or the release phase only, or during both Ca2+ uptake and release phases. The areas of the test tension transients were compared with those of the two control tension transients. It was found that halothane depressed the caffeine-induced tension transient either during the uptake phase or the combined-uptake-and-release phase but not during the release phase. The halothane-induced depression was dose-dependent, reversible, and comparable to the depression observed in intact isolated papillary muscles. We conclude that halothane could induce myocardial depression by inhibiting Ca2+ uptake by the sarcoplasmic reticulum.

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Authors and Affiliations

  1. Department of Anesthesiology, University of Washington, School of Medicine, 98195, Seattle, Washington, USA

    Judy Y. Su & W. Glenn L. Kerrick

  2. Department of Physiology and Biophysics, University of Washington, School of Medicine, 98195, Seattle, Washington, USA

    Judy Y. Su & W. Glenn L. Kerrick

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  1. Judy Y. Su
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  2. W. Glenn L. Kerrick
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Additional information

Supported by Research Grants HL 20754 and AM 17081 from the National Institutes of Health and a Research Starter Grant from the Pharmaceutical Manufactures Association Foundation. Halothane was supplied by Ayerst Laboratories, Inc.

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Su, J.Y., Kerrick, W.G.L. Effects of halothane on caffeine-induced tension transients in functionally skinned myocardial fibers. Pflugers Arch. 380, 29–34 (1979). https://doi.org/10.1007/BF00582608

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  • DOI: https://doi.org/10.1007/BF00582608

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