Summary
Smooth muscle cells of the guinea-pig sphincter pupillae were fixed in the presence of 3% lanthanum nitrate. Although only small amounts of lanthanum were found in plasmalemmal vesicles, large deposits were present within a membrane system resembling sarcoplasmic reticulum. The significance of these findings is discussed in relation to the use of lanthanum nitrate as an extracellular tracer.
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References
Bannister, L. H.: Lanthanum as an intracellular stain. J. Microscopy96, 413–419 (1972)
Bloom, W., Fawcett, D. W.: A textbook of histology, 9th ed. London: W. B. Saunders Co. 1970
Burnstock, G.: Structure of smooth muscle and its innervation. In: Smooth muscle. E. Bülbring, A. Brading, A. Jones, and T. Tomita, editors. London: Edward Arnold Publishers Ltd., 1970
Devine, C. E., Simpson, F. O., Bertaud, W. S.: Surface features of smooth muscle cells from the mesenteric artery and vas deferens. J. Cell Sci.8, 427–443 (1971)
Devine, C. E., Somlyo, A. P.: Ultrastructure of vascular smooth muscle (VSM) studied with lanthanum. Fed. Proc.29, 455 (Abstr.) (1970)
Devine, C. E., Somlyo, A. V., Somlyo, A. P.: Sarcoplasmic reticulum and excitation-contraction coupling in mammalian smooth muscles. J. Cell Biol.52, 690–718 (1972)
Doggenweiler, C. F., Frenk, S.: Staining properties of lanthanum on cell membranes. Proc. nat. Acad. Sci. (Wash.)53, 425–430 (1965)
Gabella, G.: Caveolae intracellulares and sarcoplasmic reticulum in smooth muscle. J. Cell Sci.8, 601–609 (1971)
Hodgson, B. J., Kidwai, A. M., Daniel, E. E.: Uptake of lanthanum by smooth muscle. Canad. J. Physiol. Pharmacol.50, 730–733 (1972)
Lane, N. J., Treherne, J. E.: Lanthanum staining of neurotubules in axons from cockroach ganglia. J. Cell Sci.7, 217–231 (1970)
Lesseps, R. J.: The removal by phospholipase C of a layer of La3+-staining material external to the cell membrane in embryonic chick cells. J. Cell Biol.34, 173–183 (1967)
Luft, J. H.: Ruthenium red and violet. II. Fine structural localization in animal tissues. Anat. Rec.171, 369–416 (1971)
Nishihara, H.: Some observations on the fine structure of the guinea-pig taenia coli after incubation in hypertonic solution. J. Anat. (Lond.)107, 101–114 (1970)
Overton, J.: Localized lanthanum staining of the intestinal brush border. J. Cell Biol.38, 447–452 (1968)
Revel, J. P., Karnovsky, M. J.: Hexagonal array of subunits in intercellular junctions. J. Cell Biol.33, C7. (1967)
Rubio, R., Sperelakis, N.: Penetration of horseradish peroxidase into the terminal cisternae of frog skeletal muscle fibres and blockade of caffeine contracture by Ca++ depletion. Z. Zellforsch.124, 57–77 (1972)
Somlyo, A. P., Somlyo, A. V.: Vascular smooth muscle. I. Normal structure, pathology, biochemistry and biophysics. Pharmacol. Rev.20, 197–272 (1968)
Uehara, Y., Burnstock, G.: Postsynaptic specialization of smooth muscle at close neuromuscular junctions in the guinea pig sphincter pupillae. J. Cell Biol.53, 849–853 (1972)
Venable, J. H., Coggeshall, R.: A simplified leadcitrate stain for use in electronmicroscopy. J. Cell Biol.25, 407–408 (1965)
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This study was supported by grants from the Australian Research Grants Committee and the National Heart Foundation of Australia. G. R. Campbell is the holder of a Post-Doctoral Fellowship with the National Heart Foundation of Australia. The authors are most grateful to Dr. R. Purves and to Dr. J. Chamley for their constructive criticisms of the manuscript.
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Campbell, G.R., Uehara, Y. & Burnstock, G. Lanthanum nitrate in a smooth muscle membrane system. Z.Zellforsch 147, 157–161 (1974). https://doi.org/10.1007/BF00582790
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DOI: https://doi.org/10.1007/BF00582790