Summary
The oxygen consumption together with lactic acid production and concentration of ATP, ADP, and creatinephosphate was measured during exercise and recovery on an isolated dog gastrocnemius.
Oxygen debt contraction and payment follow an exponential path with a half reaction time of about 20 sec. The concentration of ATP and ADP at steady state seem to be unaffected by the intensity of the exercise when this is submaximal and no appreciable production of lactic acid takes place. The concentration of creatinephosphate in muscle at steady state decreases with the intensity of the exercise. The ratio of the oxygen consumption at steady state to the alactic oxygen debt is identified with the speed constant of the resynthesis of phosphagen in muscle; the half reaction time of this process is 17–20 sec. The total alactic oxygen debt amounts to about 50 ml/kg of muscle. These figures are in good agreement with earlier data found in man.
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References
Belitzer, V. A., andE. T. Tsibakowa: Mechanism of phosphorilation during respiration. Biokhimiya4, 516–535 (1939).
Carlson, F. D., andA. Siger: The creatine phosphoryl transfer reaction in iodoacetate-poisoned muscle. J. gen. Physiol.43, 301–313 (1959).
Cerretelli, P., eI. Brambilla: Cinetica della contrazione di un debito di O2 nell'uomo. Boll. Soc. ital. Biol. sper.34, 679–682 (1958).
Chance, B.: Energy transfer and conservation in the respiratory chain. In: Haematic enzymes, pp. 597–624, ed. byJ. E. Falk, R. Lemberg, andR. K. Morton. New York: Pergamon 1961.
—, andB. Hess: Metabolic control mechanism. J. biol. Chem.234, 2404–2427 (1959).
—, andW. R. Williams: The respiratory chain and oxidative phosphorilation. Advanc. Enzymol.17, 65–134 (1956).
Gercken, G., u.P. Hürter: Stationäre Metabolitkonzentrationen im insuffizienten Säugetierherzen nach Monojodacetat und Natriumflorid-Vergiftung. Pflügers Arch. ges. Physiol.292, 100–117 (1966).
Green, D. E.: Electron transport and oxidative phosphorilation. Advanc. Enzymol.21, 73–129 (1951).
Henry, F. M.: Aerobic oxygen consumption and alactic debt in muscular work. J. appl. Physiol.3, 427–438 (1951).
—, andJ. de Moor: Metabolic efficiency of exercise in relation to work load at constant speed. J. appl. Physiol.2, 481–487 (1950).
——: Lactic and alactic oxygen consumption in moderate exercise of graded intensity. J. appl. Physiol.8, 608–614 (1956).
Jöbsis, F. F.: Basic processes in cellular respiration. In: Handbook of Physiology, Section 3: Respiration, Vol. 1. Amer. Physiol. Soc. Washington, D.C., pp. 63–124 (1964).
Lardy, H. A., andH. Wellmann: Oxidative phosphorilations: role of inorganic phosphate and acceptor systems in control of metabolic rates. J. biol. Chem.195, 215–224 (1952).
Lehninger, A. L., andC. L. Wadkins: Oxidative phosphorilation. Ann. Rev. Biochem.31, 47–48 (1962).
Maréchal, G.: Le métabolisme de la phosphorylcréatine et de l'adénosine triphosphate durant la contraction musculaire. Ed. Arscia-Bruxelles-Libraire Maloine, Paris 1964.
Margaria, R.: An outline for setting significant tests of muscular performance. Arch. Fisiol.64, 37–44 (1965).
Margaria, R.: Energy sources for aerobic and anaerobic work. In: Exercise at Altitude. Excerpta Medica Foundation (1967) (Ed.R. Margaria).
—,P. Cerretelli, andF. Mangili: Balance and kinetics of anaerobic energy release during strenous exercise in man. J. appl. Physiol.19, 623–628 (1964).
——,P. E. di Prampero, C. Massari, andG. Torelli: Kinetics and mechanism of oxygen debt contraction in man. J. appl. Physiol.18, 371–377 (1963).
—, andH. T. Edwards: The sources of energy in muscular work performed in anaerobic conditions. Amer. J. Physiol.108, 341–348 (1934).
——, andD. B. Dill: The possible mechanism of contracting and paying the oxygen debt and the role of lactic acid in muscular contraction. Amer. J. Physiol.106, 689–715 (1933).
—,F. Mangili, F. Cuttica, andP. Cerretelli: The kinetics of the oxygen consumption at the onset of muscular exercise in man. Ergonomics8, 49–54 (1965).
De Moor, J.: Individual differences in oxygen debt curves related to mechanical efficiency and sex. J. appl. Physiol.6, 460–466 (1954).
Piiper, J., P. E. di Prampero, andP. Cerretelli: Oxygen debt and high energy phosphates in the gastrocnemius muscle of the dog. Amer. J. Physiol. (in press) (1968).
Sacks, J., andM. G. Cleland: Absence of phosphate interchanges in ripetitive muscular contraction. Amer. J. Physiol.198, 300–302 (1960).
Thorn, W., G. Pfleiderer, R. A. Frowein u.I. Ross: Stoffwechsel-Vorgänge im Gehirn bei akuter Anoxie, akuter Ischämie und in der Erholung. Pflügers Arch. ges. Physiol.261, 334–360 (1955).
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This work has been supported in part by the Italian National Council of Sciences (CNR).
We are greatly indebted to Dr.J. Piiper for his useful criticism and help during the preparation of this paper.
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di Prampero, P.E., Margaria, R. Relationship between O2 consumption, high energy phosphates and the kinetics of the O2 debt in exercise. Pflugers Arch. 304, 11–19 (1968). https://doi.org/10.1007/BF00586714
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DOI: https://doi.org/10.1007/BF00586714