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Mécanismes de l'excrétion d'acide urique chez le rat

Mechanisms of the renal excretion of uric acid in the rat

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Summary

The renal excretion of uric acid was studied in unanesthetized rats by clearance methods at different levels of plasma concentrations, of urine flow and of urinary pH. The plasma concentrations were increased by priming the animal either i.v., or i.v. and i.p., and by infusing uric acid.

At physiological plasma levels (0.6–1.5 mg-%), only 16% of the filtered amount of uric acid was excreted. Thus, uric acid was extensively reabsorbed. When the plasma levels were increased up to 15–20 mg-% the fraction of the filtered amount excreted in the urine increased, but never exceeded 100%, even in animals subjected to a decrease of GFR by constriction of the aorta and to mannitol diuresis.

Tubular secretion of uric acid in the rat was suggested by the persistence of urinary excretion at very low plasma levels.

A difference between two strains of rats with respect to renal uric acid excretion was observed.

Résumé

L'excrétion rénale de l'acide urique a été étudiée chez le rat éveillé par des mesures de clearance, à différents taux plasmatiques d'acide urique et dans différentes conditions de débit et de pH urinaires.

Le taux plasmatique d'acide urique a été augmenté par une injection initiale soit i.v., soit i.v. et i.p., suivie d'une perfusion i.v. d'acide urique dissous dans du THAM.

Au taux plasmatique d'acide urique d'un rat normal (0,6–1,5 mg-%), 16% seulement de la quantité d'acide urique filtrée est excrétée, preuve d'une réabsorption tubulaire importante. Avec l'augmentation du taux plasmatique, ce pourcentage augmente, sans dépasser 100 aux taux plasmatiques atteints (15–20 mg-%), et ceci quelles que soient les conditions expérimentales choisies (diurèse osmotique, abaissement opératoire de la filtration glomérulaire).

Une excrétion d'acide urique persistante, aux taux plasmatiques très bas et inférieurs au seuil d'apparition extrapolé suggère l'existence d'un mécanisme de sécrétion tubulaire d'acide urique concommittant.

Deux souches de rats de type Wistar se distinguent quant à l'excrétion d'acide urique.

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  1. Institut de Pharmacologie de l'Université de Lausanne, 21, rue du bugnon, CH-1005, Lausanne, Suisse

    J. F. Boudry

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Les travaux ont bénéficié du soutien financier du Fonds National Suisse de la Recherche Scientifique (Crédits no. 4983′3 et 3′44′68).

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Boudry, J.F. Mécanismes de l'excrétion d'acide urique chez le rat. Pflugers Arch. 328, 265–278 (1971). https://doi.org/10.1007/BF00586830

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  • DOI: https://doi.org/10.1007/BF00586830

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