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Activation of vascular smooth muscle of rat aorta by noradrenaline and depolarization: Two different mechanisms

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Summary

  1. 1.

    Contraction of helical strips of the rat aorta was induced by 3 μg/ml of noradrenaline or by potassium-rich solution (80–120 mM K). The resulting amplitudes of contraction were maximal for these respective ways of activation and showed almost the same value in this preparation.

  2. 2.

    The noradrenaline-induced tension, but not the contraction induced by depolarization, decreased, when the hydrogen ion concentration of the bath solution was increased. Furthermore, the response to noradrenaline was more sensitive to a blockade of the adrenergic alpha-receptors or a variation of the bath temperature.

  3. 3.

    Since, after the application of papaverine, the amplitudes of contraction produced by noradrenaline or depolarization were diminished to the same degree, papaverine probably acts on the later steps of the activation-contraction mechanism.

  4. 4.

    When verapamil (iproveratril) was applied in order to inhibit excitationcontraction coupling, tension development after depolarization was influenced to a higher degree than noradrenaline-induced contraction.

  5. 5.

    The fact that it is possible to inhibit the response to depolarization without substantial influence on the response to noradrenaline andvice versa led to the conclusion that in the rat aorta depolarization on the one hand and noradrenaline on the other hand act via different mechanisms of activation.

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References

  1. Ahlquist, R. P.: A study of the adrenotropic receptors. Amer. J. Physiol.153, 586–600 (1948).

    Google Scholar 

  2. Albert, A.: Ionization, pH and biological activity. Pharmacol. Rev.4, 136 to 167 (1952).

    Google Scholar 

  3. Axelsson, J., Wahlström, B., Johansson, B., Jonsson, O.: Influence of the ionic environment on spontaneous electrical and mechanical activity of the rat portal vein. Circulat. Res.21, 609–618 (1967).

    Google Scholar 

  4. Biamino, G., Kruckenberg, P., Wessel, H. J.: Über Beziehungen zwischen Tonus und elektrischer Aktivität der glatten Gefäßmuskulatur. Pflügers Arch.315, 212–231 (1970).

    Google Scholar 

  5. Briggs, A. H.: Calcium movements during potassium contracture in isolated rabbit aortic strips. Amer. J. Physiol.203, 849–852 (1962).

    Google Scholar 

  6. Bygdeman, S., Euler, U. S. v.: The effect of respiratory acidosis upon the peripheral vascular reactivity to noradrenaline in the cat. Acta physiol. scand.54, 138–146 (1962).

    Google Scholar 

  7. Davignon, J., Lorenz, R. R., Shepherd, J. T.: Response of the human umbilical artery to changes in transmural pressure. Amer. J. Physiol.209, 51–59 (1965).

    Google Scholar 

  8. Fleckenstein, A., Döring, H. J., Kammermeier, H.: Einfluß von Beta-Rezeptorenblockern und verwandten Substanzen auf Erregung, Kontraktion und Energiestoffwechsel der Myokardfaser. Klin. Wschr.46, 343–351 (1968).

    Google Scholar 

  9. Ferrari, M.: Interactions between Ca and some myolytic agents on depolarized vascular smooth muscle. J. Pharm. Pharmacol.22, 71–72 (1970).

    Google Scholar 

  10. Godfraind, T., Kaba, A.: Blockade or reversal of the contraction induced by calcium and adrenaline in depolarized arterial smooth muscle. Brit. J. Pharmacol.36, 549–560 (1969).

    Google Scholar 

  11. Griebel, L., Peiper, U., Wende, W., Glaser, B., Wasner, E.: Aktivierung des isolierten Gefäßmuskelpräparates durch Noradrenalin, Vasopressin bzw. Depolarisation bei stufenweiser Blockade der alpha-Rezeptoren. Naunyn-Schmiedebergs Arch. Pharmak.270, 248–261 (1971).

    Google Scholar 

  12. Grün, G., Fleckenstein, A., Tritthart, H.: Elektromechanische Entkoppelung durch “muskulotrope” Relaxantien an der Uterusmuskulatur. Naunyn-Schmiedebergs Arch. Pharmak.264, 239–240 (1969).

    Google Scholar 

  13. Hinke, J. A. M.: Calcium requirements for noradrenaline and high potassium ion contraction in arterial smooth muscle. In: W. M. Paul, E. E. Daniel, C. M. Kay and G. Mockton: Muscle. Oxford: Pergamon Press 1965.

    Google Scholar 

  14. —, Wilson, M. L., Burnham, S. C.: Calcium and the contractility of arterial smooth muscle. Amer. J. Physiol.206, 211–215 (1964).

    Google Scholar 

  15. Hiraoka, M., Yamagishi, S., Sano, T.: Role of calcium ions in the contraction of vascular smooth muscle. Amer. J. Physiol.214, 1084–1089 (1968).

    Google Scholar 

  16. Holman, M. E.: Electrophysiology of vascular smooth muscle. Physiol. Rev.61, 137–177 (1969).

    Google Scholar 

  17. —, Kasby, C. B., Suthers, M. B., Wilson, J. A. F.: Some properties of the smooth muscle of rabbit portal vein. J. Physiol. (Lond.)196, 111–132 (1968).

    Google Scholar 

  18. Hudgins, P. M.: Some drug effects on calcium movements in aortic strips. J. Pharmacol. exp. Ther.170, 303–310 (1969).

    Google Scholar 

  19. —, Weiss, G. B.: Differential effects of calcium removal upon vascular smooth muscle contraction induced by norepinephrine, histamine and potassium. J. Pharmacol. exp. Ther.159, 91–97 (1968).

    Google Scholar 

  20. ——: Characteristics for45Ca binding in vascular smooth muscle. Amer. J. Physiol.217, 1310–1315 (1970).

    Google Scholar 

  21. Jhamandas, K. H., Nash, C. W.: Effects of inorganic anions on the contractility of vascular smooth muscle. Canad. J. Physiol. Pharmacol.45, 675–682 (1967).

    Google Scholar 

  22. Johansson, B., Jonsson, O., Axelsson, J., Wahlström, B.: Electrical and mechanical characteristics of vascular smooth muscle response to norepinephrine and isoproterenol. Circulat. Res.21, 619–633 (1967).

    Google Scholar 

  23. Keatinge, W. R.: Mechanism of adrenergic stimulation of mammalian arteries and its failure at low temperatures. J. Physiol. (Lond.)174, 184–205 (1964).

    Google Scholar 

  24. Keatinge, W. R.: Reversal of electrical response of arterial smooth muscle to noradrenaline in calcium-deficient solution. J. Physiol. (Lond.)198, 20 P (1968).

    Google Scholar 

  25. Kimizuka, H., Koketsu, K.: Binding of calcium ion to a lecithin film. Nature (Lond.)196, 995–996 (1962).

    Google Scholar 

  26. Kirchheim, H., Gross, R.: Das Verhalten der Nierendurchblutung und des Nierenumfanges bei Blutdrucksteigerungen durch doppelseitigen Carotisverschluß oder Schrittmacher-Tachykardie. Untersuchungen zur Autoregulation der Nierendurchblutung am wachen Hund. Pflügers Arch.320, 79–96 (1970).

    Google Scholar 

  27. Ko, K.-C., Gimeno, A. L., Berman, D. A.: Electrical and contractile properties of isolated rat atria in bufferfree medium as influenced by changes in pH. Experientia (Basel)24, 137–139 (1968).

    Google Scholar 

  28. Kutscha, W., Pauschinger, P., Brecht, K.: Der Einfluß der H-Ionen auf Elektrolytgehalt, Membranpotential und Kontraktion tonischer und phasischer Skeletmuskeln. Pflügers Arch. ges. Physiol.280, 1–21 (1964).

    Google Scholar 

  29. Lewis, G. R.: The importance of ionization in the activity of sympathetic amines. Brit. J. Pharmacol.9, 488–495 (1954).

    Google Scholar 

  30. Lundholm, L., Mohme-Lundholm, E.: Dissociation of contraction and stimulation of lactic acid production in experiments on smooth muscle under anaerobic conditions. Acta physiol. scand.57, 111–124 (1963).

    Google Scholar 

  31. Mainwood, G. W., Lee, S. L.: The hyperpolarizing effect of hydrogen ions on transmembrane potential in frog skeletal muscle. Canad. J. Physiol. Pharmacol.46, 151–157 (1968).

    Google Scholar 

  32. McNeill, R. J., Stark, R. D., Greenway, C. V.: Intestinal vasoconstriction after hemorrhage: role of vasopressin and angiotensin. Amer. J. Physiol.219, 1342–1347 (1970).

    Google Scholar 

  33. Mekata, H., Niu, H.: Electrical and mechanical responses of coronary artery smooth muscle to catecholamines. Jap. J. Physiol.19, 599–608 (1969).

    Google Scholar 

  34. Murphy, R. A., Bohr, D. F., Newman, D. L.: Arterial actomyosin: Mg, Ca, and ATP ion dependencies for ATPase activity. Amer. J. Physiol.217, 666–673 (1969).

    Google Scholar 

  35. Peiper, U., Griebel, L., Wende, W.: Unterschiedliche Temperaturabhängigkeit der Gefäßmuskelkontraktion nach Aktivierung durch Kalium-Depolarisation bzw. Noradrenalin. Pflügers Arch.324, 67–78 (1971).

    Google Scholar 

  36. Peiper, U., Wende, W., Wullstein, H. K.: Der Einfluß von Temperatur, Vorspannung und Propranolol auf die Noradrenalinwirkung am isolierten Gefäßstreifen der Rattenaorta. Pflügers Arch.305, 167–176 (1969).

    Google Scholar 

  37. Peiper, U., Wende, W.: Wirkung der extracellulären Wasserstoffionen-Konzentration auf die adrenerge Aktivierung des Gefäßmuskels. Pflügers Arch.314, 14–26 (1970).

    Google Scholar 

  38. ——: Adrenerge Aktivierung und Kaliumkontraktur des Gefäßmuskels. Pflügers Arch.316, R 22 (1970).

    Google Scholar 

  39. Robison, G. A., Sutherland, E. W.: Sympathin E, Sympathin I and the intracellular level of cyclic AMP. Circulat. Res.27, Suppl. 1, 147–161 (1970).

    Google Scholar 

  40. Shibata, S., Briggs, A. H.: Mechanical activity of vascular smooth muscle under anoxia. Amer. J. Physiol.212, 981–984 (1967).

    Google Scholar 

  41. Sparrow, W. P., Maxwell, L. C., Ruegg, J. C., Bohr, D. F.: Preparation of a calcium ion-sensitive actomyosin from arteries. Amer. J. Physiol.219, 1366–1371 (1970).

    Google Scholar 

  42. Speden, R. N.: Excitation of vascular smooth muscle. In: E. Bülbring, A. Bradig, A. Jones and T. Tomita: Smooth muscle, pp. 558–588. London: E. Arnold Ltd 1970.

    Google Scholar 

  43. Su, C., Bevan, J. A.: Dissociation by SY-14 of potassium induced depolarization and contraction in vascular smooth muscle. Fed. Proc.22, 308 (1963).

    Google Scholar 

  44. Van Breemen, C.: Blockade of membrane calcium fluxes by Lanthanum in relation to vascular smooth muscle contractility. Arch. int. Physiol. Biochem.77, 710–716 (1969).

    Google Scholar 

  45. Voth, D., Schipp, R., Agsten, M., Schürmann, K., Kohlhardt, M., Dudek, J.: Untersuchungen über den Einfluß des Kationenmilieus und verschiedener Pharmaka auf die Kontraktilität und Autorhythmik eines spontan aktiven glatten Gefäßmuskels in vitro. Arch. Kreisl.-Forsch.60, 364–387 (1969).

    Google Scholar 

  46. Waugh, W. H.: Calcium and contraction of arterial smooth muscle. In: W. M. Paul, E. E. Daniel, C. M. Kay and G. Monckton: Muscle. Oxford: Pergamon Press 1965.

    Google Scholar 

  47. Wende, W., Peiper, U.: Wechselwirkung von Kalium und Noradrenalin auf die Spannungsentwicklung des isolierten Gefäßmuskels. Pflügers Arch.320, 133–141 (1970).

    Google Scholar 

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  1. Physiologisches Institut der Universität Würzburg, Würzburg, Germany

    U. Peiper, L. Griebel & W. Wende

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  1. U. Peiper
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  2. L. Griebel
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  3. W. Wende
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Peiper, U., Griebel, L. & Wende, W. Activation of vascular smooth muscle of rat aorta by noradrenaline and depolarization: Two different mechanisms. Pflugers Arch. 330, 74–89 (1971). https://doi.org/10.1007/BF00588736

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  • DOI: https://doi.org/10.1007/BF00588736

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