Summary
Blood flow and activity of oxidative and glycolytic enzymes was studied in slow- and fast-twitch muscles in kittens and in slow and fast muscles in chicks in the course of postnatal development. Soleus (in kittens) and anterior latissimus dorsi (in chicks) were used as representatives of slow muscles, gastrocnemius (in kittens) and posterior latissimus dorsi (in chicks) as representatives of fast-twitch muscles.
Blood flow measured by133Xe clearance was similar in soleus and gastrocnemius (20 ml/100 g x min) during the first week of life. Later, blood flow increased slightly in soleus, and bccame considerably lower in gastrocnemius. The first significant difference was observed in 5-week-old kittens, where the blood flow was 2.4 times higher in soleus than in gastrocnemius. In adult cats blood flow (measured by photoelectric drop-counter) was 39.8±12.7 ml/100 g tissue x min in soleus, and 7.0±1.5 ml/100 g x min in gastrocnemius.
The number of open capillaries per muscle fibre (studied in histological sections after India ink injection) was always higher in muscles with higher blood flow, and in 35-day-old kittens was significantly higher in soleus than in gastrocnemius.
The activity of the two oxidative enzymes measured (citrate synthase and 3-hydroxyacetyl-CoA-dehydrogenase) was higher in soleus than in gastrocnemius in one-day-old kittens, and this proportion did not change substantially during further development. The activity of both enzymes increased in both muscles after 21 day of age.
The activity of glycolytic enzymes (lactate dehydrogenase and triosephosphodehydrogenase) was similar in both muscles in kittens up to 21 days; thereafter, their activity increased much more rapidly in gastrocnemius than in soleus.
In chickens, in which electromyographic activity, in both slow and fast muscles decreased during postnatal development, the activity of oxidative enzymes decreased as well, the decrease being greater after the 4th day in fast muscle in slow. Similarly, blood flow which was the same in both muscles until 8 days of age decreased more in the course of further development in fast than in slow muscles.
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Hudlická, O., Pette, D. & Staudte, H. The relation between blood flow and enzymatic activities in slow and fast muscles during development. Pflugers Arch. 343, 341–356 (1973). https://doi.org/10.1007/BF00595821
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DOI: https://doi.org/10.1007/BF00595821