Summary
A colony staining method was used to isolate mutants inAspergillus niger which showed altered glucose oxidase induction. The mutants were isolated under weakly or non-inducing conditions. A stable glucose-oxidase-negative mutant and a series of overproducing mutants were found. Among the overproducing mutants, different phenotypes were found with respect to glucose oxidase induction. The mutants were tested for glucose oxidase production in surface and submerged cultures, indicating a fair correspondence between those methods. From the characteristics of the mutants it can be concuded that oxygen- and carbonsource-dependent induction are mediated by different factors.
Similar content being viewed by others
References
Bensadoun A, Weinstein D (1976) Assay of proteins in the presence of interfering materials. Anal Biochem 70:241–250
Bos CJ, Debets AJM, Swart K, Huybers A, Kobus G, Slakhorst SM (1988) Genetic analysis and construction of master strains for assignment of genes to six linkage groups inAspergillus niger. Curr Genet 14:437–443
Fiedurek J, Rogalski J, Ilczuk Z, Leonowicz A (1986) Screening and mutagenesis of moulds for the improvement of glucose oxidase production. Enzyme Microb Technol 8:734–736
Heinrich M, Rehm HJ (1982) Formation of gluconic acid at low pH-values by free and immobilizedAspergillus niger cells during citric acid fermentation. Eur J Appl Microbiol Biotechnol 15:88–92
Itzhaki RF, Gill OM (1964) A microbiuret method for estimating proteins. Anal Biochem 9:401–410
Kriechbaum m, Heilmann HJ, Wientjes FJ, Hahn M, Jany JD, Gassen HG, Sharif F, Alaeddinoglu G (1989) Cloning and DNA sequence analysis of the glucose oxidase gene fromAspergillus niger NRRL-3. FEBS Lett 255:63–66
Kundu PN, Das A (1985) A note on crossing experiments withAspergillus niger for the production of calcium gluconate. J Appl Bacteriol 59:1–5
Markwell J, Frakes LG, Brott EC, Osterman J, Wagner FW (1989)Aspergillus niger mutants with increased glucose oxidase production. Appl Microbiol Biotechnol 30:166–169
Mischak H, Kubicek CP, Röhr M (1985) Formation and location of glucose oxidase in citric acid producing mycelia ofAspergillus niger. Appl Microbiol Biotechnol 21:27–31
Pontecorvo G, Roper JA, Hemmons LM, MacDonald KD, Bufton AWJ (1953) The genetics ofAspergillus nidulans. Adv Genet 5:141–239
Ramasamy K, Kelley RL, Reddy CA (1985) Lack of lignin degradation by glucose oxidase negative mutants ofPhanerochaete chrysosporium. Biochem Biophys Res Commun 131:436–441
Röhr M, Kubicek CP, Kominek J (1983) Gluconic acid. In: Rehm HJ, Reed G (eds) Biotechnology III. Verlag Chemie, Weinheim, pp 456–465
Vishniac W, Santer M (1957) The thiobacilli. Bacteriol Rev 21:195–213
Witteveen CFB, Busink R, Vondervoort P van de, Dijkema C, Swart K, Visser J (1989)l-Arabinose andd-xylose catabolism inAspergillus niger. J Gen Microbiol 135:2163–2171
Zetelaki KZ (1970) The role of aeration and agitation in the production of glucose oxidase in submerged culture II. Biotechnol Bioeng 12:379–397
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cor Witteveen, F.B., van de Vondervoort, P., Swart, K. et al. Glucose oxidase overproducing and negative mutants ofAspergillus niger . Appl Microbiol Biotechnol 33, 683–686 (1990). https://doi.org/10.1007/BF00604938
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00604938