Summary
A semi-intact eye cup preparation was developed which maintains visual responses and enables intracellular recordings to be made from the optic lobes of the crayfish compound eye.
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1.
Sustaining fibers (SFs) were impaled with lucifer yellow electrodes near their entrance to the second optic neuropil (where the SFs originate).
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2.
Corneal receptive fields were determined and the SFs identified based on the previous work of Wiersma and Yamaguchi (1966). Following identification of each cell, the dendritic morphology was observed with lucifer yellow iontophoresis and subsequent fluorescence microscopy (Fig. 2).
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3.
An individual SF possesses a dendritic arborization restricted to that portion of the medulla corresponding to its corneal receptive field (Fig. 3). Therefore, the position of the dendritic tree combined with the retinotopic organization within the distal optic neuropils determine each SF's visual receptive field.
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Abbreviations
- SFs :
-
sustaining fibers
- EPSPs :
-
excitatory postsynaptic potentials
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Kirk, M.D., Waldrop, B. & Glantz, R.M. The crayfish sustaining fibers. J. Comp. Physiol. 146, 175–179 (1982). https://doi.org/10.1007/BF00610235
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DOI: https://doi.org/10.1007/BF00610235