Abstract
Using the shrinking droplet method and simultaneous perfusion of the peritubular capillaries the isotonic reabsorption of Ringer's solution from the papillary collecting ducts was measured. Under control conditions the volume reabsorption from the papillary collecting ducts wasJ v±SE=2.6±0.1 · 10−5 cm3 · cm−2 · s−1. In rats which were on low Na+ diet,J v increased to 127%, and in adrenalectomized animals it decreased to 34% of the control value. Three hours after application of aldosterone in the adrenalectomized animalsJ v was partially restored to 63% of control rats. Amiloride 10−4 M, added to the luminal perfusate, produced a strong inhibition ofJ v (to 32% of control). Acetazolamide, 10−4 M, added to both perfusates, reducedJ v very strongly (to 40% of control), while omission of bicarbonate reduced it only to 77% of control. Acetazolamide, added to bicarbonate-free perfusates, did not result in a significant further reduction ofJ v. The data indicate that the Na+ reabsorption from the papillary collecting duct is controlled by mineralocorticoids. Furthermore, they suggest the existence of two transport mechanisms in the luminal cell membrane: 1. An amiloride-sensitive entry step and 2. an entry step via a Na+−H+-countertransport mechanism, the latter being less important.
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References
Deetjen, P., Kramer, K.: Die Abhängigkeit des O2-Verbrauchs der Niere von der Na-Rückresorption. Pflügers Arch.273, 636–650 (1961)
Diezi, J., Michoud, P., Aceves, J., Giebisch, G.: Micropuncture study of electrolyte transport across papillary collecting duct of the rat. Am. J. Physiol.224, 623–634 (1973)
Edelman, I. S.: Candidate mediators in the action of aldosterone on Na+ transport. In: Membrane Transport Processes, (ed. J. F. Hoffman), Vol. 1, pp. 125–140. New York: Raven 1978
Funder, J. W., Feldmann, D., Edelman, I. S.: The role of plasma binding and receptor specificity in the mineralocorticoid action of aldosterone. Endocrinology92, 994–1004 (1972)
Gertz, K. H.: Transtubuläre Natriumchloridflüsse und Permeabilität für Nichtelektrolyte im proximalen und distalen Konvolut der Rattenniere. Pflügers Arch.276, 336–356 (1963)
Gross, J. B., Kokko, J. P.: Effects of aldosterone and potassium-sparing diuretics on electrical potential differences across the distal nephron. J. Clin. Invest.59, 82–89 (1977)
Gross, J. B., Imai, M., Kokko, J. P.: A functional comparison of the cortical collecting tubule and the distal convoluted tubule. J. Clin. Invest.55, 1284–1294 (1975)
Györy, A. Z.: Reexamination of the split oil droplet method as applied to kidney tubules. Pflügers Arch.324, 328–343 (1971)
Hierholzer, K., Lange, S.: The effects of adrenal steroids on renal function. In: MTP International Review of Science. Kidney and Urinary Tract Physiology. Physiol. Ser. I, Vol. 6. (A. C. Guyton and K. Thurau, eds.), pp. 273–333. London: Butterworth 1974
Hierholzer, K., Wiederholt, M., Holzgreve, H., Giebisch, G., Klose, R. M., Windhager, E. E.: Micropuncture study of renal transtubular concentration gradients of sodium and potassium in adrenalectomized rats. Pflügers Arch.285, 193–210 (1965)
Iyengar, R., Mailman, D. S., Sachs, G.: Purification of distinct plasma membranes from canine renal medulla. Am. J. Physiol.234, F247-F254 (1978)
Knox, F. G.: Regulation of collecting duct sodium reabsorption. In: New Aspects of Renal Function. (H. G. Vogel and K. J. Ullrich, eds.), pp. 181–183. Amsterdam: Excerpta Medica 1978
O'Neil, R. G., Boulpaep, E.: Active ion transport properties of rabbit renal collecting tubules: Influence of amiloride and amphotericin B. Fed. Proc.37, 728 (1978)
O'Neil, R. G., Helman, S. I.: Transport characteristics of renal collecting tubules: Influence of DOCA and diet. Am. J. Physiol.233, F 544-F 588 (1977)
Radtke, H. W., Rumrich, G., Kinne-Saffran, E., Ullrich, K. J.: Dual action of acetazolamide and furosemide on proximal volume absorption in the rat kidney. Kidney Int.1, 100–105 (1972)
Sonnenberg, H.: Effect of adrenalectomy on medullary collecting-duct function in rats before and during blood volume expansion. Pflügers Arch.368, 55–62 (1977)
Stoner, L. C., Burg, M. B., Orloff, J.: Ion transport in cortical collecting tubule, effect of amiloride. Am. J. Physiol.227, 453–459 (1974)
Uhlich, E., Baldamus, C. A., Ullrich, K. J.: Einfluß von Aldosteron auf den Natriumtransport in den Sammelrohren der Säugerniere. Pflügers Arch.308, 111–126 (1969)
Ullrich, K. J.: Summary of discussion on Dr. Sonnenberg's presentation. In: New Aspects of Renal Function. (H. G. Vogel and K. J. Ullrich, eds.), pp. 184–185. Amsterdam: Excerpta Medica 1978
Ullrich, K. J., Radtke, H. W., Rumrich, G.: The role of bicarbonate and other buffers on isotonic fluid absorption in the proximal convolution of the rat kidney. Pflügers Arch.330, 149–161 (1971)
Ullrich, K. J., Capasso, G., Rumrich, G., Papavassiliou, F., Klöss, S.: Coupling between proximal tubular transport processes. Studies with ouabain, SITS and HCO −3 -free solutions. Pflügers Arch.368, 245–252 (1977)
Ullrich, K. J., Frömter, E., Murer, H.: How is the proximal isotonic absorption inhibited by carbonic anhydrase inhibitors. In: Diuretics in research and clinics (W. Siegenthaler, R. Beckerhoff, and W. Vetter, eds.), pp. 2–7. Stuttgart: Thieme 1977
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Ullrich, K.J., Papavassiliou, F. Sodium reabsorption in the papillary collecting duct of rats. Pflugers Arch. 379, 49–52 (1979). https://doi.org/10.1007/BF00622904
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DOI: https://doi.org/10.1007/BF00622904