Summary
A study was made on the permeability of cerebral blood vessels to albumin during development. Fluorescent labeled bovine serum albumin was injected into a tail vein of newborn, young and adult rats and into the umbilical artery of embryos from the fifteenth to the twenty-first day of pregnancy. The distribution of the tracer was ascertained by means of fluorescence microscopy. In the brains of the embryos and postnatal rats the fluorescent albumin was strictly confined to the lumen of the blood vessels, while considerable extravascular passage was observed in subcutaneous tissue. The results indicate that in rat embryos the cerebral blood vessels are impermeable to albumin at least as early as the fifteenth day after fertilization.
Zusammenfassung
Es wurde eine Untersuchung der Permeabilität der Hirngefäße für Albumin im Verlaufe der Entwicklung durchgeführt. Fluorescenz-markiertes Rinder-Serumalbumin wurde in eine Schwanzvene von neugeborenen, jungen und erwachsenen Ratten sowie in die Umbilicalarterie von Embryonen zwischen dem 15. und 21. Schwangerschaftstag injiziert. Die Verteilung der markierten Substanz wurde fluorescenzmikroskopisch nachgewiesen. In den Gehirnen embryonaler und postnataler Ratten war das fluorescenzmarkierte Albumin streng auf das Lumen der Blutgefäße beschränkt, während im subeutanen Gewebe eine erhebliche extravasale Passage nachgewiesen werden konnte. Die Befunde sprechen dafür, daß bei Rattenembryonen die Hirngefäße zumindest ab dem 15. Schwangerschaftstag für Albumin undurchlässig sind.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abdou, J. A., andH. Tarver: Plasma protein. I. Loss from circulation and catabolism to carbon dioxide. J. biol. Chem.190, 769–780 (1951).
Behnsen, G.: Über die Farbstoffspeicherung im Zentralnervensystem der weißen Maus in verschiedenen Alterszuständen. Z. Zellforsch.4, 515–538 (1927).
Bodenheimer, T. T., andM. W. Brightman: Perivascular space and the blood-brain barrier to peroxidase. Anat. Rec.157, 351 (1967).
Broman, T., andO. Steinwall: Blood-brain barrier. In: Neuropathology. Ed.J. M. Minckler. New York: McGraw-Hill (in press).
Dobbing, J.: The blood-brain barrier. Physiol. Rev.41, 130–138 (1961).
Edström, R.: Recent developments of the blood-brain barrier concept. Int. Rev. Neurobiol.7, 403–416 (1964).
Everett, N. B., andB. I. Simmons: Measurement and radioautographic localization of albumin in rat tissues after intravenous administration. Circulat. Res.6, 307–313 (1958).
Grazer, F. M., andC. D. Clemente: Developing blood-brain barrier to trypan blue. Proc. Soc. exp. Biol. (N. Y.)74, 758–760 (1957).
Gröntoft, D.: Intracranial haemorrage and blood-brain barrier problems in the new-born. Acta path. microbiol. scand. Suppl. C (1954).
—: The permeability to P32 in different regions of the brain of new-born and adult rabbits. Acta path. microbiol. scand.63, 481–492 (1965).
Himwich, W. A., J. C. Petersen, andM. Allen: Hematoencephalic exchange as a function of age. Neurology (Minneap.)7, 705–710 (1957).
Humphrey, J. H., A. Neuberger, andP. Perkins: Observations on the presence of plasma proteins in skin and tendon. Biochem. J.66, 390–399 (1957).
Klatzo, I., J. Miquel, C. Tobias, andW. Haymaker: Effects of alpha-particle radiation on the rat brain, including vascular permeability and glycogen studies. J. Neuropath. exp. Neurol.20, 459–483 (1961).
—, andR. Otenasek: The application of fluorescein labelled serum proteins (FLSP) to the study of vascular permeability in the brain. Acta neuropath. (Berl.)2, 144–160 (1962).
Klatzo, I., W. Wisniewski, andD. Smith: Obervations on penetration of serum proteins into the central nervous system. Progr. Brain Res.15, 73–88 (1965).
Lajtha, A.: The brain barrier system. In: Neurochemistry (2nd ed.), p. 399. Ed.K. A. C. Elliot, I. H. Page andJ. H. Quastel. Springfield, Ill.: Ch. C. Thomas 1962.
Mancini, R. E., O. Vilar, C. Gómez, J. M. Dellacha, O. W. Davidson, andA. Castro: Histological study of distribution of fluorescent serum protein in connective tissue. J. Histochem. Cytochem.9, 356–362 (1961).
Reese, T. S., andM. J. Karnovsky: Fine structural localization of a blood-brain barrier for exogenous peroxidase. J. Cell Biol.34, 207–217 (1967).
Stern, L., andR. Peyrot: Le fonctionnement de la barrière hématoencéphalique aux divers stades de développment chez les diverses espèces animales. C. R. Soc. Biol. (Paris)96, 1124 (1927).
Steinwall, O., andI. Klatzo: Selective vulnerability of blood-brain barrier in chemically induced lesions. J. Neuropath. exp. Neurol.25, 542–559 (1966).
Waelsch, H.: The turnover of components of the developing brain; the blood-brain barrier. In: Biochemistry of the developing nervous system. Ed.H. Waelsch. New York: Academic Press 1955.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Olsson, Y., Klatzo, I., Sourander, P. et al. Blood-brain barrier to albumin in embryonic new born and adult rats. Acta Neuropathol 10, 117–122 (1968). https://doi.org/10.1007/BF00691305
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00691305