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Yeast cell-cycle mutantcdc21 is a temperature-sensitive thymidylate auxotroph

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Summary

Genetic tests with the yeast cell-cycle mutantcdc21 isolated by Hartwell indicate that the CDC21 gene in yeast is the same as the TMP1 gene, whose mutant alleles confer an auxotrophic requirement for thymidine-5′-monophosphate (dTMP). Yeast strains carryingcdc21 can grow at 37° in the presence of dTMP provided that they are permeable to this compound. The gene is shown to be linked toade2 on Chr. XV, and a case of intragenic complementation betweencdc21 and anothertmp1 allele is reported.

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Authors and Affiliations

  1. Division of Microbiology, National Institute for Medical Research, Mill Hill, NW7 1AA, London, U.K.

    J. C. Game

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  1. J. C. Game
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Communicated by W. Gajewski

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Game, J.C. Yeast cell-cycle mutantcdc21 is a temperature-sensitive thymidylate auxotroph. Molec. Gen. Genet. 146, 313–315 (1976). https://doi.org/10.1007/BF00701257

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  • DOI: https://doi.org/10.1007/BF00701257

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