Abstract
AnAspergillus sp., isolated from a rubbish dump, produced 10.6 IU ml-1 xylanase activity. Two xylanases were recognized and each was purified to homogeneity by two-stage chromatography on DEAE-and CM-Sepharose. Xylanase I had a pI of 7.2 and anM r of 26 kDa whereas xylanase II had a pI of 4.7 and anM r of 21 kDa. At 50°C, xylanase I was stable for 2.5 h but xylanase II was only stable for 1 h.
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P. Khanna is with the National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440 020, India. S. Sivakami Sundari and N. Jothi Kumar are with the National Environmental Engineering Research Institute, Madras Zonal Laboratory, CSIR Madras Complex, Taramani 600 113, India.
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Khanna, P., Sundari, S.S. & Kumar, N.J. Production, isolation and partial purification of xylanases from anAspergillus sp.. World J Microbiol Biotechnol 11, 242–243 (1995). https://doi.org/10.1007/BF00704661
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DOI: https://doi.org/10.1007/BF00704661