Summary
In this study the proliferative (stem?) cells within the parenchyma of the normal “resting” breast were characterised by the ultrastructural examination of 60 mitotic cells. The parenchyma consists of epithelial and myoepithelial cells plus a few intraepithelial lymphocytes and macrophages. The majority of mitotic cells were randomly distributed throughout the lobules with a few present in ducts. In all cases the cells were identified as luminally positioned polarised epithelial cells. The proliferating cells had similar cytoplasmic features and were indistinguishable from adjacent interphase epithelial cells. No evidence was found for the division of subluminal epithelial or myoepithelial cells. These observations would be consistant with a single cell type giving rise to both epithelial and myoepithelial cells.
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Anderson TJ, Ferguson DJP, Raab GM (1982) Cell turnover in the “resting” human breast: the influence of parity, contraceptive pill, age and laterality. Br J Cancer 46:376–382
Baessler R (1970) The morphology of hormone-induced structural changes in the female breast. Curr Top Pathol 51:1–89
Bennett DC (1980) Morphogenesis of branching tubules in cultures of cloned mammary epithelial cells. Nature 285:657–659
Ferguson DJP, Anderson TJ (1981a) Morphological evaluation of cell turnover in relation to the menstrual cycle in the “resting” human breast. Br J Cancer 44:177–181
Ferguson DJP, Anderson TJ (1981b) Ultrastructural observations on cell death by apoptosis in the “resting” breast. Virchows Arch [Pathol Anat] 393:193–203
Ferguson DJP, Anderson TJ (1981c) A technique for identifying areas of interest in human breast tissue before embedding for electron microscopy. J Clin Pathol 34:1187–1189
Ferguson DJP (1985) Intraepithelial lymphocytes and macrophages in the normal breast. Virchows Arch [Pathol Anat] 407:369–378
Ormerod EJ, Rudland PS (1984) Cellular composition and organization of ductal buds in developing rat mammary glands: evidence for morphological intermediates between epithelial and myoepithelial cells. Am J Anat 170:631–652
Ozzello L (1971) Ultrastructure of the human mammary gland. Pathol Annual 6:1–59
Potten CS (1981) Celt replacement in epidermis (keratoporesis) via discrete units of proliferation. International Review of Cytology 69:271–318
Rudland PS, Ormerod EJ, Paterson F (1980) Stem cells in rat mammary gland development and cancer: a reviw. J Roy Soc Med 73:437–442
Russo IH, Ireland M, Isenberg W, Russo J (1976a) Ultrastructural description of three different epithelial cell types in rat mammary gland (Abstract). Proc Elec Micr Soc Am 34:146–147
Russo J, Isenberg W, Ireland M, Russo IH (1976b) Ultrastructural changes in the mammary epithelial cell population during neoplastic development induced by a chemical carcinogen (Abstract). Proc Elec Mic Soc Am 34:250–251
Salazar H, Tobon H (1974) Morphologic changes of the mammary gland during development, pregnancy and lactation. In: Josimovich JB (ed) Lactogenic hormones, fetal nutrition and lactation. John Wiley and Sons, New York, pp 221–277
Short RV, Drife JO (1977) The aetiology of mammary cancer in man and animals. Symph Zool Soc Lond 41:211–230
Smith EA, Monaghan P, Neville AM (1984) Basal clear cells of the normal human breast. Virchows Arch [Pathol Anat] 402:319–329
Stirling JW, Chandler JA (1976) The fine structure of the normal, resting terminal ductal lobular unit of the female breast. Virchows Arch [Pathol Anat] 372:205–226
Toker C (1967) Observations on the ultrastructure of a mammary ductule. J Ultrastr Res 21:9–25
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Ferguson, D.J.P. Ultrastructural characterisation of the proliferative (stem?) cells within the parenchyma of the normal “resting” breast. Vichows Archiv A Pathol Anat 407, 379–385 (1985). https://doi.org/10.1007/BF00709985
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DOI: https://doi.org/10.1007/BF00709985