Skip to main content
SpringerLink
Log in

Glucose and lactate concentrations during activity in the leopard frog,Rana pipiens

  • Published:
Journal of comparative physiology Aims and scope Submit manuscript

Summary

  1. 1.

    Resting blood metabolite concentrations were determined at 19 different times of the daily cycle (15°C, LD12:12). Blood glucose values for 151 frogs determined over a 6-month period yielded a mean level of 24.5±3.0 mg·100 ml−1 blood (mg%). Blood lactate values for 132 animals yielded a mean level of 31.0±12.3 mg%.

  2. 2.

    Blood glucose concentrations over a 24-hour period showed no diel cycle. Blood lactate values over a 24-hour period were much more erratic, but with a distinct peak one h after the onset of scotophase (1900 hrs CST), suggestive of a diurnal cycle.

  3. 3.

    No correlations were found between normal blood glucose and lactate concentrations and weight, sex, or season.

  4. 4.

    Faradic stimulation of 15- and 30 min duration were equally efficient in eliciting maximal lactate concentrations. A 2-min period of shocking was significantly less effective.

  5. 5.

    Stressed (i.e., due to strenuous exercise) blood metabolite concentrations forR. pipiens were determined 0.0, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, and 8.5 hrs following a 30-min period of Faradic stimulation from 1200 to 1230 hrs CST. Blood glucose levels increased from 20.9 to 57.2 mg% and blood lactate values increased from 18.6 to 99.5 mg% during a period of two hrs following stimulation; normal levels were again attained 4.0 hrs after the end of stimulation.

  6. 6.

    Resting liver, muscle, and total body lactate concentrations were 20.9±3.5, 76.8±17.5, and 44.7±12.5 mg 100 g−1 tissue wt. (mg%), respectively. Stressed tissue lactate levels were determined 0.0, 1.0, 3.0, 4.0, and 7.5 hrs following the Faradic stimulation period. Immediately following the end of stimulation, liver, muscle, and total body lactate concentrations increased by factors of 4.6 (from 20.9 to 95.6 mg%), 3.2 (from 76.8 to 244.0 mg%), and 4.0 (from 44.7 to 180.8 mg%), respectively. After one h of recovery, all three lactate concentrations were reduced abruptly to approximately one-half the peak value and were maintained for several hrs before return to normal values after 7.5 hrs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barlow, O. W., Vigor, W. N., Peck, R. I.: The action of insulin on the frog, the influence of dosage, temperature, excision of the liver, administration of glucose, sodium bicarbonate, and calcium gluconate on the reaction of the frog to insulin. J. Pharm. exp. Therap.41, 229–243 (1931)

    Google Scholar 

  • Bennett, A. F., Licht, P.: Anaerobic metabolism during activity in lizards. J. comp. Physiol.81, 277–288 (1972)

    Google Scholar 

  • Bennett, A. F., Licht, P.: Relative contributions of anaerobic and aerobic energy production during activity in amphibia. J. comp. Physiol.87, 351–360 (1973)

    Google Scholar 

  • Bennett, A. F., Licht, P.: Anaerobic metabolism during activity in amphibians. Comp. Biochem. Physiol.48A, 319–327 (1974)

    Google Scholar 

  • Black, E. C., Robertson, A. C., Parker, R. R.: Some aspects of carbohydrate metabolism in fish. In: Comperative physiology of carbohydrate metabolism in heterothermic animals (Martin, A. W., ed.), p. 89–214. Seattle: Univ. of Washington Press 1961

    Google Scholar 

  • Christiansen, J., Penney, D.: Anaerobic glycolysis and lactic acid accumulation in cold submergedRana pipiens. J. comp. Physiol.87, 237–245 (1973)

    Google Scholar 

  • Duncan, D. B.: Multiple range and multiple F-tests. Biometrisc11, 1–6 (1955)

    Google Scholar 

  • Fromm, P. O., Johnson, R. E.: The respiratory metabolism of frogs as related to season. J. cell. comp. Physiol.45, 343–360 (1955)

    Google Scholar 

  • Guimond, R. W., Hutchison, V. H.: The effect of temperature and photoperiod on gas exchange in the leopard frog,Rana pipiens. Comp. Biochem. Physiol.27, 177–195 (1968)

    Google Scholar 

  • Harris, J. A.: Seasonal variation in some hematological characteristics ofRana pipiens. Comp. Biochem. Physiol.43A, 975–989 (1972)

    Google Scholar 

  • Hermansen, B., Jorgensen, C. B.: Blood glucose in male toads (Bufo bufo): Annual variation and hormonal regulation. Gen. comp. Endocr.12, 313–321 (1969)

    Google Scholar 

  • Hutchison, V. H., Whitford, W. G., Kohl, M.: The effect of photoperiod on daily rhythms of oxygen consumption in the tropical toad,Bufo marinus. Z. vergl. Physiol.75, 367–382 (1971)

    Google Scholar 

  • Jones, D. R., Mustafa, T.: The lactacid oxygen debt in frogs after one hour's apnoea in air. J. comp. Physiol.85, 15–24 (1973)

    Google Scholar 

  • Mazzocco, P.: Ocilationes periodicas del metabolismo hidrocarbonado en el sapoBufo arenarum (Hensel). Rev. Soc. argent. Biol.14, 330–334 (1938)

    Google Scholar 

  • Miller, M. R.: Carbohydrate metabolism in amphibians and reptiles. In: Comparative physiology of carbohydrate metabolism in heterothermic animals (Martin, A. W., ed.), p. 125–144. Seattle: Univ. of Washington Press 1961

    Google Scholar 

  • Mizell, S.: Seasonal changes in energy reserves in the common frog,Rana pipiens. J. cell. comp. Physiol.66, 251–258 (1965)

    Google Scholar 

  • Scott, E. L., Kleitman, N.: Sugar in the blood of the common frog. Amer. J. Physiol.55, 355–361 (1921)

    Google Scholar 

  • Seiden, G.: The response of the pancreatic islands of the frogRana pipiens to alloxan. Anat. Rec.91, 187–197 (1945)

    Google Scholar 

  • Seymour, R. S.: Physiological correlates of forced activity and burrowing in the spadefoot toad,Scaphiopus hammondii. Copeia1, 1–22 (1973)

    Google Scholar 

  • Smith, C. L.: Seasonal changes in blood sugar, body fat, liver glycogen, and gonads in the common frog,Rana temporaria. J. exp. Biol.26, 412–429 (1950)

    Google Scholar 

  • Tenney, S. M., Tenney, J. B.: Quantitative morphology of cold-blooded lungs: Amphibia and reptilia. Resp. Physiol.9, 197–215 (1970)

    Google Scholar 

  • Turney, L. D., Hutchison, V. H.: Metabolic scope, oxygen debt, and diurnal oxygen consumption cycle of the leopard frog,Rana pipiens. Comp. Biochem. Physiol.49A, 583–601 (1974)

    Google Scholar 

  • Ultsch, G. R.: A theoretical and experimental investigation of the relationship between metabolic rate, body size, and oxygen exchange capacity. Resp. Physiol.18, 143–160 (1973)

    Google Scholar 

  • Whitford, W. G., Hutchison, V. H.: Body size and metabolic rate in salamanders. Physiol. Zool.40, 127–133 (1967)

    Google Scholar 

  • Wright, P. A.: Blood sugar studies in the bullfrogRana catesbeiana. Endocrinology64, 551–558 (1959)

    Google Scholar 

  • Wurster, D. H.: Comparative studies on the blood glucose and pancreatic islets of the salamander,Taricha torosa, and a lizard,Eumeces obsoletus. Ph.D. dissertation, Stanford University (1958)

Download references

Author information

Authors and Affiliations

  1. Department of Zoology, University of Oklahoma, 73069, Norman, Oklahoma, USA

    Victor H. Hutchison & L. Douglas Turney

Authors
  1. Victor H. Hutchison
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Douglas Turney
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hutchison, V.H., Turney, L.D. Glucose and lactate concentrations during activity in the leopard frog,Rana pipiens . J Comp Physiol B 99, 287–295 (1975). https://doi.org/10.1007/BF00710369

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00710369

Keywords

Search

Navigation