Skip to main content
SpringerLink
Log in

The intermediate filament proteins of rabbit vascular smooth muscle: immunofluorescent studies of desmin and vimentin

  • Papers
  • Published:
Journal of Muscle Research & Cell Motility Aims and scope Submit manuscript

Summary

The localization of desmin and vimentin in rabbit vascular smooth muscle was studied using the fluorescent antibody technique. Desmin and vimentin were present in the smooth muscle cells of the portal anterior mesenteric vein, renal vein and renal artery. All endothelial cells and the smooth muscle cells of the abdominal aorta and main pulmonary artery contained only vimentin. The results suggest that desmin and vimentin are differentially distributed in rabbit vascular smooth muscle and that both may occur in a single blood vessel.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • ASHTON, F. T., SOMLYO, A. V. & SOMLYO, A. P. (1975) The contractile apparatus of vascular smooth muscle: Intermediate high voltage stereo electron microscopy.J. molec. Biol. 98, 17–29.

    Google Scholar 

  • BENNETT, G. S., FELLINI, S. A. & HOLTZER, H. (1978a) Immunofluorescent visualization of 100 Å filaments in different cultured chick embryo cell types.Differentiation 12, 71–82.

    Google Scholar 

  • BENNETT, G. S., FELLINI, S. A., CROOP, J. M., OTTO, J. J., BRYAN, J, & HOLTZER, H. (1978b) Differences among 100 Å filament subunits from different cell types.Proc. natn. Acad. Sci., U.S.A. 75, 4364–8.

    Google Scholar 

  • BENNETT, G. S., FELLINI, S. A., TOYAMA, Y. & HOLTZER, H. (1979) Redistribution of intermediate filament subunits during skeletal myogenesis and maturationin vitro.J. Cell Biol. 82, 577–84.

    Google Scholar 

  • BERNER, P. F., SOMLYO, A. V. & SOMLYO, A. P. (1981) Hypertrophy-induced increase of intermediate filaments in vascular smooth muscle.J. Cell Biol. 88, 96–101.

    Google Scholar 

  • CAMPBELL, G. R., CHAMLEY-CAMPBELL, J., GROSCHEL-STEWART, U., SMALL, J. V. & ANDERSON, P. (1979) Antibody staining of 10 nm (100 Å) filaments in cultured smooth, cardiac and skeletal muscle cells.J. Cell Sci. 37, 303–22.

    Google Scholar 

  • COOKE, P. H. & CHASE, R. H. (1971) Potassium chloride-insoluble myofilaments in vertebrate smooth muscle cells.Expl Cell Res. 66, 417–25.

    Google Scholar 

  • COOKE, P. H. (1976) A filamentous cytoskeleton in vertebrate smooth muscle fibers.J. Cell Biol. 68, 539–56.

    Google Scholar 

  • DEYL, Z., MACEK, K., ADAM, M, & VANCIKOVA. (1980) Studies on the chemical nature of elastin fluorescence.Biochim. Biophys. Acta 625, 248–54.

    Google Scholar 

  • FELLINI, S. A., BENNETT, G. S. & HOLTZER, H. (1978a) Selective binding of antibody against gizzard 10 nm filaments to different cell types in myogenic cultures.Am. J. Anat. 153, 451–7.

    Google Scholar 

  • FELLINI, S. A., BENNETT, G. S., TOYAMA, Y. & HOLTZER, H. (1978b) Biochemical and immunological heterogeneity of 100 Å filament subunits from different chick cell types.Differentiation 12, 59–69.

    Google Scholar 

  • FRANK, E. D. & WARREN, L. (1981) Aortic smooth muscle cells contain vimentin instead of desmin.Proc. natn. Acad. Sci., U.S.A. 78, 3020–4.

    Google Scholar 

  • FRANKE, W. W., SCHMID, E., OSBORN, M. & WEBER, K. (1978) Different intermediate-sized filaments distinguished by immunofluorescence microscopy.Proc. natn. Acad. Sci., U.S.A. 75, 5034–8.

    Google Scholar 

  • FRANKE, W. W., SCHMID, E., WINTER, S., OSBORN, M. & WEBER, K. (1979a) Widespread occurrence of intermediate-sized filaments of the vimentin-type in cultured cells from diverse vertebrates.Expl Cell Res. 123, 25–46.

    Google Scholar 

  • FRANKE, W. W., DENK, H., SCHMID, E., OSBORN, M. & WEBER, K. (1979b) Ultrastructural biochemical and immunological characterization of Mallory bodies in livers of griseofulvin-treated mice: Fimbriated rods of 14–20 nm filaments containing prekeratin polypeptides.Lab. Invest. 40, 207–20.

    Google Scholar 

  • FRANKE, W. W., SCHMID, E., OSBORN, M. & WEBER, K. (1979c) The intermediate-sized filaments of human endothelial cells.J. Cell Biol. 81, 570–80.

    Google Scholar 

  • GABBIANI, G., SCHMID, E., WINTER, S., CHAPONNIER, C., DeCHASTONAY, C., VANDERKERCKHOVE, J., WEBER, K. & FRANKE, W. W. (1981) Vascular smooth muscle cells differ from other smooth muscle cells: Predominance of vimentin filaments and a specific-type actin.Proc. natn. Acad. Sci., U.S.A. 78, 298–302.

    Google Scholar 

  • GABELLA, G. (1979) Hypertrophic smooth muscle. IV. Myofilaments, intermediate filaments and some mechanical properties.Cell Tissue Res. 201, 278–88.

    Google Scholar 

  • GARD, D. & LAZARIDES, E. (1980) The synthesis and distribution of desmin and vimentin during myogenesisin vitro.Cell 19, 263–75.

    Google Scholar 

  • GOLDSTEIN, G., SLIZYS, I. S. & CHASE, M. W. (1961) Studies on fluorescent antibody staining. I. Non-specific fluorescence with fluorescein-coupled sheep anti-rabbit globulins.J. exp. Med. 114, 89–110.

    Google Scholar 

  • HOLTZER, H., SANGER, J., ISHIKAWA, H. & STRAHS, H. (1973) Selected topics in skeletal myogenesis.Cold Spring Harb. Symp. quant. Biol. 37, 549–60.

    Google Scholar 

  • HOLTZER, H., FELLINI, S. A., RUBINSTEIN, N., CHI, J. & STRAHS, K. (1976) Cells, myosin and 100 Å filaments. InCell Motility (edited by GOLDMAN, R., Pollard, T. and Rosenbaum, J.), pp. 823–839. Cold Spring Harbor Laboratories.

  • HOLTZER, H., BENNETT, G. S., TAPSCOTT, S. J., CROOP, J. M., DLUGOSZ, A. & TOYAMA, Y. (1981) Changes in intermediate-sized filaments during myogenesis and neurogenesis. InInternational Cell Biology (edited by SCHWEIGER, J.), pp. 293–305. Berlin, Heidelberg, New York: Springer Verlag.

    Google Scholar 

  • HUBBARD, B. D. & LAZARIDES, E. (1979) Co-purification of actin and desmin from chicken smooth muscle and their copolymerizationin vitro to intermediate filaments.J. Cell Biol. 80, 166–82.

    Google Scholar 

  • HUIATT, T. W., ROBSON, R. M., ARAKAWA, N. & STROMMER, M. H. (1980) Desmin from avian smooth muscle.J. biol. Chem. 255, 6981–9.

    Google Scholar 

  • ISHIKAWA, H., BISCHOFF, R. & HOLTZER, H. (1968) Mitosis and intermediate-sized filaments in developing skeletal muscle.J. Cell Biol. 38, 538–55.

    Google Scholar 

  • JOHNSON, P. & YUN, J. S. (1980) Intermediate filaments of bovine pulmonary artery smooth muscle: Distribution, isolation and polypeptide composition.Int. J. Biochem. 11, 143–54.

    Google Scholar 

  • LAEMMLI, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophase T4.Nature, Lond. 227, 680–5.

    Google Scholar 

  • LAZARIDES, E. (1980) Intermediate filaments as mechanical integrators of cellular space.Nature, Lond. 283, 249–56.

    Google Scholar 

  • LAZARIDES, E. & HUBBARD, B. D. (1976) Immunological characterization of the 100 Å filaments from muscle cells.Proc. natn. Acad. Sci., U.S.A. 73, 4344–8.

    Google Scholar 

  • SCHMID, E., TAPSCOTT, S., BENNETT, G. S., CROOP, J., FELLINI, S. A., HOLTZER, H. & FRANKE, W. W. (1979) Differential location of different types of intermediate-sized filaments in various tissues of the chicken embryo.Differentiation 15, 27–40.

    Google Scholar 

  • SMALL, J. V. & SOBIESZEK, A. (1977) Studies on the function and composition of the 10-nm (100 Å) filaments of vertebrate smooth muscle.J. Cell Sci. 23, 243–68.

    Google Scholar 

  • SOMLYO, A. P., DEVINE, C. E., SOMLYO, A. V. & RICE, R. V. (1972) Filament organization in vertebrate smooth muscle.Phil. Trans. R. Soc. Lond. Ser. B 265, 223–9.

    Google Scholar 

  • SOMLYO, A. P. & SOMLYO, A. V. (1975) Ultrastructure of smooth muscle. InMethods of Pharmacology (edited by DANIEL, E. E. and PATON, D. M.), Vol. 3, pp. 3–43. New York: Plenum.

    Google Scholar 

  • TAPSCOTT, S. J., BENNETT, G. S., TOYAMA, Y., KLEINBART, F. & HOLTZER, H. (1981) Intermediate filament proteins in developing chick spinal cord.Devl Biol. 86, 40–54.

    Google Scholar 

  • UEHARA, Y., CAMPBELL, G. R. & BURNSTOCK, G. (1971) Cytoplasmic filaments in developing and adult vertebrate smooth muscle.J. Cell Biol. 50, 484–97.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Pennsylvania Muscle Institute, University of Pennsylvania, School of Medicine, 19104, Philadelphia, Pennsylvania, USA

    Peter F. Berner

  2. Wistar Institute of Anatomy and Biology, University of Pennsylvania, 36th and Spruce Streets, 19104, Philadelphia, Pennsylvania, USA

    Evan Frank, Howard Holtzer & Andrew P. Somlyo

Authors
  1. Peter F. Berner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Evan Frank
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Howard Holtzer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Andrew P. Somlyo
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Berner, P.F., Frank, E., Holtzer, H. et al. The intermediate filament proteins of rabbit vascular smooth muscle: immunofluorescent studies of desmin and vimentin. J Muscle Res Cell Motil 2, 439–452 (1981). https://doi.org/10.1007/BF00711969

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00711969

Keywords

Search

Navigation