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Isolation of a melibiose-binding protein from human spleen

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Abstract

A melibiose-binding protein was isolated from human spleen by serial affinity chromatography on lactose-, mannose-, and melibiose-Sepharose. The purified protein agglutinated rabbit erythrocytes and re-bound to melibiose, but did not bind to murine nor human laminin. The protein was composed of ∼58 kDA, 32 kDa and 26 kDa polypeptides. The polypeptides were detected in buffy coat cell extracts and they were synthesizedin vitro by B lymphoblastoid cells. The polypeptides did not react with anti-galaptin, anti-C-reactive protein, anti-amyloid P, anti-keratin, and anti-rat lung lectin 29 sera. The 58 kDa polypeptide reacted very weakly with anti-core-specific lectin serum and reacted with anti-IgG serum. The data suggest that the major protein isolated is an anti-Galαl → 6 immunoglobulin.

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Abbreviations

ME:

mercaptoethanol

PMSF:

phenylmethylsufonyl fluoride

HEPES:

4-(2-hydroxyethyl)-1-piperazine ethanosulfonate

PBS:

0.01m PO4, 0.12m NaCl, pH 7.3

TBS:

0.1m NaCl, 0.05m Tris, 0.05% NaN3, 0.01m CaCl2, 0.001m MgCl2, pH 7.3

BSA:

bovine serum albumin

GSI:

Griffonia simplicifolia I

SDS-PAGE:

sodium dodecylsulfate-polyacrylamide gel electrophoresis

References

  1. Cummings RD, Smith DF (1992)BioEssays 14:849–56.

    Google Scholar 

  2. Hirabayashi J (1993)Trends Glycosci Glycotechnol 5:251–70.

    Google Scholar 

  3. Lotan R (1992) InGlycoconjugates: Composition, Structure, and Function (Allen HJ, Kisailus EC eds) pp. 635–71. New York: Marcel Dekker, Inc.

    Google Scholar 

  4. Drickamer K (1988)J Biol Chem 263:9557–60.

    Google Scholar 

  5. Drickamer K (1989)Ciba Found Symp 145:45–61.

    Google Scholar 

  6. Leffler H, Masiarz FR, Barondes SH (1989)Biochemistry 28:9222–29.

    Google Scholar 

  7. Barondes SH, Castronovo V., Cooper DNWet al. (1994)Cell 76:597–98.

    Google Scholar 

  8. Barondes SH, Gitt MA Leffler H, Cooper DNW (1988)Biochimie 70:1627–32.

    Google Scholar 

  9. Caron M, Bladier D, Joubert R (1990)Int J Biochem 22:1379–85.

    Google Scholar 

  10. Hirabayashi J, Kasai K-i. (1993)Glycobiology 3:297–304.

    Google Scholar 

  11. Bezouska K, Crichlow, GV, Rose JM, Taylor ME, Drickamer K. (1991)J Biol Chem 266:11604–9.

    Google Scholar 

  12. Allen HJ, Gottstine S, Sharma A, DiCioccio RA, Swank RT, Hua L (1991)Biochemistry 30:8904–10.

    Google Scholar 

  13. Sharma A, DiCioccio RA, Allen HJ (1992)Glycobiology 2:285–292.

    Google Scholar 

  14. Sharma A, Chemelli R, Allen HJ (1990)Biochemistry 29:5309–14.

    Google Scholar 

  15. Allen HJ, Johnson EAZ (1977)Carbohydr Res 58:253–65.

    Google Scholar 

  16. Ahmed H, Allen HJ, DiCioccio RA (1991)Carbohydr Res 213:321–24.

    Google Scholar 

  17. DiCioccio RA, Brown KS (1988)Biochem Genet 26:401–20.

    Google Scholar 

  18. Laemmli UK (1970)Nature 227:680–85.

    Google Scholar 

  19. Bonner WM, Lasky RA (1974)Eur J Biochem 46:83–88.

    Google Scholar 

  20. Ahmed H, Allen HJ, Sharma, A, Matta, K (1990)Biochemistry 29:5315–19.

    Google Scholar 

  21. Knibbes RN, Perini F, Goldstein IJ (1989)Biochemistry 28:6379–89.

    Google Scholar 

  22. Galili U, Shohet SB, Kobrin E, Stults CLM, Macher, BA (1988)J Biol Chem 263:17755–62.

    Google Scholar 

  23. Galili U, Basbaum CB, Shohet SB, Buehler J, Macher BA (1987)J Biol Chem 262:4683–88.

    Google Scholar 

  24. Sandrin MS, Vaughan, HA, Dabkowski PL, McKenzie IF (1993)Proc Natl Acad Sci USA 90:11391–95.

    Google Scholar 

  25. Good AH, Cooper DK, Malcolm AJ, Ippolito RM, Koren E, Neethling FA, Ye Y, Zudhi N, Lamontagne LR (1992)Transplant Proc 24:559–62.

    Google Scholar 

  26. Towbin H, Rosenfelder G, Wieslander J, Avila J, Rojas M, Szarfman A, Esser K, Nowack H, Timpl R (1987)J Exp Med 166:419–32.

    Google Scholar 

  27. Joubert-Caron R, Caron M, Bochet P, Chadli A, Delaporte P, Schuller E, Bladier D (1994)Int J Biochem 26:813–23.

    Google Scholar 

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Authors and Affiliations

  1. Department of Gynecologic Oncology, Roswell Park Cancer Institute, 14263, Buffalo, New York, USA

    Ashu Sharma, Hafiz Ahmed & Howard J. Allen

Authors
  1. Ashu Sharma
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  2. Hafiz Ahmed
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  3. Howard J. Allen
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Sharma, A., Ahmed, H. & Allen, H.J. Isolation of a melibiose-binding protein from human spleen. Glycoconjugate J 12, 17–21 (1995). https://doi.org/10.1007/BF00731864

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  • DOI: https://doi.org/10.1007/BF00731864

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