Abstract
A method of counterflow isotachophoresis of proteins on cellulose acetate films is suggested. To produce a counterflow of fluid, an electroendosmotic flow formed in the film during passage of an electric current through it was used. During isotachophoresis of proteins in the presence of ampholytes, the proteins are concentrated on the boundary between the leading and closing ions and, at the same time, they are fractionated by electrophoretic mobility. Microanalysis of protein mixtures in dilute solutions can be undertaken by this method. It is readily combined with immunodiffusion and immunoelectrophoretic detection of protein antigens.
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Abelev, G.I., Karamova, É.R. Counterflow isotachphoresis on cellulose acetate membranes role of electroendosmosis. Bull Exp Biol Med 88, 1049–1053 (1979). https://doi.org/10.1007/BF00799280
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DOI: https://doi.org/10.1007/BF00799280