Abstract
The antifungal activity of cysteine (2-amino-3-mercaptopropionic acid) on mycelial growth and the production of lanosterol, 3,β-hydroxy-lanosta-8,24-dien-21-al, 3,β-21,dihydroxy-lanosta-8,24-diene, trametenolic acid and ergosterol were investigated in solid and liquid cultures ofInonotus obliquus at cysteine concentrations of 0.9 and 9.0 mm by means of thinlayer chromatography densitometry. Cysteine elicited the production of ergosterol in all the cultures and at a concentration of 0.9 mm distinctly elicited the production of 3,β-hydroxy-lanosta-8,24-dien-21-al. At 9.0 mm, cysteine had greater antifungal activity than at 0.9 mm, decreasing growth and the production of metabolities.
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References
Abuzinadah RA, Read DJ (1988) Amino acids as nitrogen sources for ectomycorrhizal fungi: Utilization of invidual amino acids. Trans Br Mycol See 91:473–480
Adams HK, Campell MI, McCorkindale NJ (1967) Ergosterol peroxide: a fungal artefact. Nature 216:397
Cochrane VW (1958) Physiology of fungi. Wiley, New York
Daigle DJ, Cotty OJ (1991) The influence of cysteine, cysteine analogs and other amino acids on spore germination of Altenaria species. J Can Bot 69:2353–2356
Goulston G, Mercer EL Goad LJ (1975). The identification of 24-methylene-24-25-dihydrolanosterol and other possible ergosterol precursors inPhycomyces blakesleeanus andAgaricus campestris. Phytochemistry 14:457–462
Griffin DH (1981) Fungal physiology. Wiley, New York
Hirotani M, Asaka I, Ino C, Furuya T, Shiro M (1987) Ganoredic acid derivatives and ergosta-4,7,22-triene-3,6-dione fromGanoderma lucidum. Phytochemistry 26:2797–2803
Huxtable J-R (1986) Biochemistry of sulphur, Lafraconi WM (ed) Plenum Press, New York.
Kahlos K (1994a)Inonotus obliquus (Chaga fungus): in vitro culture and production of inotodiol, sterols and other secondary metabolities. In: Bajai YPS. (ed) Biotechnology in agriculture and foresty, vol 26. (Medicinal and aromatic plants VI) Springer Berlin Heidelberg New York, pp 179–197
Kahlos K (1994b) The effects of some amino acids on growth and lipid production inInonotus obliquus grownin vitro. Acta Biotechnol 14:169–179
Kahlos K, Kangas L, Hiltunen R (1987) Antitumour activity of some compounds and fractions from an n-hexane extract ofInonotus obliquus. Acta Pharm Fenn 96:33–40.
Kahlos K, Kangas L, Hiltunen R (1989) Ergosterol peroxide an active compound fromInonotus radiatus. Planta Med 55:389–390
Kahlos K, Vares T, Hiltunen R (1990) Optimization of pH level and effect of pH on secondary metabolites of two strains ofInonotus obliquus, in vitro. Planta Med 56:627
Kahlos K, Siirilä M, Hiltunen R (1991) Effects of chitosan on fungal growth and the production of lipid compounds in Inonotus obliquus. Planta Med: 56 A 16
Kahlos K, Hännikäinen T, Hiltunen R (1993) The antifungal activity of allicin and its effect on some lanostanes in vitro. XII Helsinki University Course in Drug Research, 17-18. June 1993 Espco, Finland, Abstracts p 75. The Finnish Pharmaceutical Society, Helsinki
Kermasha S, Gloetghebeur M, Monfenette A, Metche M, Rovel B (1993) Inhibitory effects of cysteine and aromatic acids on tyrosinase activity. Phytochemistry 34:349–353
Kunert J (1981) Organic sulphur sources for the growth of the dermatophyte Microsporum gypseum. Folia Microbiol 26:201–206
Minaeva VG (1970) Medicinal plants of Siberia. Navka, Novosibirsk
Naim M, Zuker I, Zehavi U, Rouseff R (1993) Inhibition of thiol compounds off flavor formation in stored orange juice. 2. Effect ofl-cysteine andn-acetyl-l-cysteine onp-vinylguaiacol formation. J Agric Food Chem 41:1359–1361
Owen HR, Wengerd D, Miller AR (1991) Culture medium pH is influenced by carbohydrate source, gelling agent, activated charcoal and medium storage method. Plant Cell Rep 10:583–586
Pandey DK, Chandra H, Tripathi NN, Dixit SN (1984) Antimycotic activity of some amino acids against dermatophytes. Arzneim Forsch 34:554–556
Sheikh YM, Djerassi C (1974) Steroids from sponges. Tetrahedron 30:4095–4103
Slaughter JC (1989) Sulphur compounds in fungi. In: Boddy L, Marchant R, Read DJ (eds) Nitrogen, phosphorus and sulphur utilization by fungi. Cambridge University Press, Cambridge, pp 91–106
Thuy NN, Galgoczy J, Novak EK (1981) Morphogenetic effect of L-cysteine on dermatophytes. Acta Microbiol Acad Sci Hung 28:347–457
Torres MR, Viladirich R, Sachis V, Canela R (1992) Influence of age and ergosterol content in mycelium of Aspergillus ochraceus. Lett Appl Microbiol 15:20–22
Turner WB (1971) Fungal metabolites. Academic Press, London
Weete JD (1973) Sterols of the fungi: distribution and biosynthesis. Phytochemistry 12:1843–1864
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Kahlos, K., Tikka, V.H. Antifungal activity of cysteine, its effect on C-21 oxygenated lanosterol derivatives and other lipids in Inonofus obliquus, in vitro. Appl Microbiol Biotechnol 42, 385–390 (1994). https://doi.org/10.1007/BF00902746
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DOI: https://doi.org/10.1007/BF00902746