Summary
The hydrocarbon degradation rate could be doubled by the addition of sophorose lipids as biosurfactants in a model system containing 10% soil and a 1.35% hydrocarbon mixture of tetradecane, pentadecane, hexadecene, 1,2,4-trimethylcyclohexane, pristane (2,6,10,14-tetramethylpentadecane) phenyldecane and naphthalene suspended in mineral salts medium. The adaptation phases for two degradation phases were shortened, and the extent of degradation and final biomass were increased. The added biosurfactants were degraded after they had facilitated degradation of all hydrocarbon components.
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References
Atlas RM, Bartha R (1973) Effects of some commercial oil herders, dispersants and bacterial inocula on Biodegradation of oil in seawater. In: Ahearn DG, Meyers SP (eds) The microbial degradation of oil pollutants. Lousiana State University publ. no. LSU-SG-73-01, Baton Rouge, pp 283–289
Bernern K-H van (1984) Experimentelle Untersuchungen zur Wirkung von Rohöl und Rohöl/Tensid-Gemischen im Ökosystem Wattenmeer. II. Eindringverhalten und Persistenz von Rohölkohlenwasserstoffen in Sedimenten nach experimenteller Kontamination. Senckenb Marit 16:13–23
Bossert I, Bartha R (1984) The fate of petroleum in soil ecosystems. In: Atlas RM (ed) Petroleum microbiology. Macmillan, New York, pp 435–473
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal Biochem 72:248–254
Cooney J (1984) The fate of petroleum pollutants in freshwater ecosystems. In: Atlas RM (ed) Petroleum microbiology. Macmillan, New York, pp 399–433
Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung (1981) 9. Lieferung H 41, Verlag Chemie, Weinheim
Hisatsuka K-I, Nakahara T, Sano N, Yamada K (1971) Formation of rhamnolipid byPseudomonas aeruginosa and its function in hydrocarbon fermentation. Agric Biol Chem 35:686–692
Hodge JD, Hofreiter BT (1962) Determination of reducing sugars and carbohydrates. In: Whistler RL, BeMiller IN (eds) Methods in carbohydrate chemistry, vol 1: Academic Press, New York, pp 380–394
Lang S, Wagner F (1987) Structure and properties of biosurfactants. In: Kosaric N, Cairns WL, Gray NCC (eds) Biosurfactants and biotechnology. Dekker, New York, pp 21–45
Mattei G, Bertrand J-C (1985) Production of biosurfactants by a mixed bacteria population grown in continuous culture on crude oil. Biotechnol Lett 7:217–222
Mattei G, Rambeloarisoa E, Giusti G, Rontani JF, Bertrand JC (1986) Fermentation procedure of a crude oil in continuous culture on seawater. Appl Microbiol Biotechnol 23:302–304
Näveke R, Tepper KP (1982) Einführung in die mikrobiologischen Arbeitsmethoden. Fischer, Stuttgart
Oberbremer A, Müller-Hurtig R (1989) Aerobic stepwise hydrocarbon degradation and formation of biosurfactants by an original soil population in a stirred reactor. Appl Microbiol Biotechnol 31:582–586
Shailubhai K (1986) Treatment of petreoleum industry oil sludge in soil. Trends Biotechnol 4:200–206
Syldatk C, Wagner F (1987) Production of biosurfactants. In: Koraric N, Cairns WL, Gray NCC (eds) Biosurfactants and biotechnology. Dekker, New York, pp 89–120
Zajic JE, Seffens W (1984) Biosurfactants. CRC Crit Rev Microbiol 5:87–107
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Oberbremer, A., Müller-Hurtig, R. & Wagner, F. Effect of the addition of microbial surfactants on hydrocarbon degradation in a soil population in a stirred reactor. Appl Microbiol Biotechnol 32, 485–489 (1990). https://doi.org/10.1007/BF00903788
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DOI: https://doi.org/10.1007/BF00903788