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Alteration in rat apolipoprotein C-III gene expression and lipoprotein composition during inflammation

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Abstract

A cloned rat apolipoprotein (apo) C-III cDNA was used as a hybridization probe to measure apo C-III mRNA levels in rats after induction of inflammation. Hepatic apo C-III mRNA levels decrease to a minimum value of 46% of normal 72 h after the induction of inflammation. The changes observed are very similar to the changes in mRNA levels of rat apo A-IV, while the hepatic mRNA levels for apo A-I and apo C-I during inflammation remain relatively constant. Alterations in apo C-III gene expression during inflammation were associated with changes in lipoprotein particle size and composition. A decrease of approximately twofold in the amount of apo C-III-containing HDL particles was found in rats 24 h after the induction of inflammation. The average size of apo C-III-containing HDL particles in rats during inflammation is significantly larger than that of the control group. A decrease in the concentration of apo A-I-containing HDL particles was also observed in these rats. The results indicate altered apolipoprotein gene expression associated with alterations in the size and composition of HDL particles.

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Authors and Affiliations

  1. Russell Grimwade School of Biochemistry, University of Melbourne, 3052, Parkville, Victoria, Australia

    Peiyan Shen & Geoffrey J. Howlett

  2. Department of Genetics, University of Melbourne, 3052, Parkville, Victoria, Australia

    Peiyan Shen

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  1. Peiyan Shen
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Shen, P., Howlett, G.J. Alteration in rat apolipoprotein C-III gene expression and lipoprotein composition during inflammation. Inflammation 17, 153–166 (1993). https://doi.org/10.1007/BF00916102

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