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Complement-dependent hemodynamic and hematologic changes in the rabbit

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Abstract

The intravenous injection of the anticomplementary protein from cobra venom, cobra factor (CoF), induces decreases in mean arterial blood pressure and circulating platelets in rabbits. The changes are rapidly reversed. Both changes require the presence of C3 and occur in rabbits genetically deficient in the sixth component of complement. The hypotensive effects of CoF were blocked by the histamine H2-receptor antagonist burimamide. An acute C3-dependent change in blood pressure and circulating platelets also was demonstrated following the intravenous injection ofS. marcescens endotoxin. However, abrogation of these acute changes by C3 depletion did not alter the extent of a second, prolonged fall in blood pressure and platelets induced byS. marcescens endotoxin occurring after 60–90 min. C3 depletion also did not alter the lethal effects of theS. marcescens endotoxin.

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Abbreviations

LPS:

lipopolysaccharide

C6:

the sixth component of complement

PAGE:

polyacrylamide-gel electrophoresis

CoF, cobra factor:

the anticomplementary protein isolated from cobra venom

C3:

the third component of complement

PAF:

platelet-activating factor

S. marcescens :

Serratia marcescens

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Authors and Affiliations

  1. Department of Immunopathology, Scripps Clinic and Research Foundation, 92037, La Jolla, California

    Richard J. Ulevitch & Charles G. Cochrane

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  1. Richard J. Ulevitch
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  2. Charles G. Cochrane
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Additional information

This work was supported by United States Public Health Service Grant AI-07007, HL 17786 and Office of Naval Research ONR 207-027. R. J. Ulevitch is the recipient of United States Public Health Service Young Investigator Pulmonary Research Grant HL 18376.

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Ulevitch, R.J., Cochrane, C.G. Complement-dependent hemodynamic and hematologic changes in the rabbit. Inflammation 2, 199–216 (1977). https://doi.org/10.1007/BF00917596

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