Abstract
Antigens derived fromEchinococcus granulosus, Taenia hydatigena andT. pisiformis cyst fluids,T. solium cysticerci,E. multilocularis protoscoleces andE. vogeli cyst membranes were examined by enzyme-linked immunosorbent assay (ELISA) and immunoelectrophoresis (IEP) using four monoclonal antibodies (mAb) toE. granulosus antigen 5 (Ag5) and antigen B (AgB). Anti-Ag5 mAbs 24.14 and 61A12 reacted strongly withT. hydatigena andT. pisiformis cyst fluids and, to a lesser degree, anti-AgB MAbs 31.15 and 39B3 also displayed some reaction with these antigens in ELISA. The formation of a modified arc 5 band between Anti-Ag5 mAbs andT. hydatigena cyst fluid (THCF) in IEP further confirms the existence of Ag5 inT. hydatigena cyst fluid. However, the inability of THCF andT. pisiformis cyst fluid (TPCF) to form an AgB band as well as that of TPCF to form an arc 5 band with mAbs in IEP does not exclusively prove the lack of AgB in THCF and TPCF or the lack of Ag5 in TPCF. The absence of a reaction of mAbs withT. solium, E. multilocularis andE. vogeli antigen preparations in ELISA or IEP would suggest that these mAbs may recognise epitopes different from those ofT. solium, E. multilocularis andE. vogeli parasites; this might be exploited for specific differentiation ofE. granulosus.
This is a preview of subscription content, log in via an institution to check access.
References
Capron A, Vernes A, Biguet J (1967) Le diagnostic immunoélectrophorétique de l'hydatidose. Le kyste hydatique du foie. Lyon Hydatidol, pp 27–40
Herbert IV, Osberg OC (1975) Serological studies on pigs experimentally infected withT. solium orT. hydatigena. J Comp Pathol 85:487–498
Jenkins DJ, Rickard MD (1985) Specific antibody responses toTaenia hydatigena, Taenia pisiformis andEchinococcus granulosus infection in dogs. Aust Vet J 62:72–78
Lightowlers MW, Liu D, Haralambous A, Rickard MD (1989) Subunit composition and specificity of the major cyst fluid antigens ofEchinococcus granulosus. Mol Biochem Parasitol 37:171–182
Liu D, Lightowlers MW, Rickard MD (1992a) Evaluation of a monoclonal antibody based competition ELISA for diagnosis of human hydatidosis. Parasitology 104:357–361
Liu D, Lightowlers MW, Rickard MD (1992b) Examination of murine antibody response to secondary hydatidosis using ELISA and immunoelectrophoresis. Parasite Immunol 14:239–248
Liu D, Rickard MD, Lightowlers MW (1992c) Further characterisation of monoclonal antibodies toEchinococcus granulosus antigen 5 and antigen B. Int J Parasitol 22:391–394
Liu D, Rickard MD, Lightowlers MW (1992d) Comparative immunoelectrophoretic analysis ofEchinococcus granulosus, Taenia hydatigena andTaenia pisiformis cyst fluid antigens by hyperimmune rabbit sera. Res Vet Sci 53:133–135
Schantz PM, Shanks D, Wilson M (1980) Serologic cross-reactions with sera from patients with echinococcosis and cysticercosis. Am J Trop Med Hyg 29:609–612
Varela-Díaz VM, Coltorti EA (1976) Immunodiagnostic techniques for human hydatidosis. Monograph 7. Pan American Zoonosis Centre. Pan American Health Organisation, Buenos Aires
Varela-Díaz VM, Gemmell MA, Williams JF (1972) Immunological responses of the mammalian host against tapeworm infections: XII. Observations on antigenic sharing betweenTaenia hydatigena andTaenia ovis. Exp Parasitol 32:96–101
Varela-Díaz VM, Coltorti EA, Rickard MD, Torres JM (1977a) Comparative antigenic characterisation ofEchinococcus granulosus andTaenia hydatigena cysts fluids by immunoelectrophoresis. Res Vet Sci 23:213–216
Varela-Díaz VM, Eckert J, Rausch RL, Coltorti EA, Hess U (1977b) Detection of theEchinococcus granulosus diagnostic arc 5 in sera from patients with surgically confirmedE. multilocularis infection. Z Parasitenkd 53:183–188
Varela-Díaz VM, Coltorti EA, D'Alessandro A (1978) Immunoelectrophoresis tests showingEchinococcus granulosus arc 5 in human cases ofEchinococcus vogeli and cysticercosis-multiple myeloma. Am J Trop Med Hyg 27:554–557
Yarzábal LA, Bout DT, Naquira FR, Capron AR (1977) Further observations on the specificity of antigen 5 ofEchinococcus granulosus. J Parasitol 63:495–499
Yong WK, Heath DD (1979) “Arc 5” antibodies in sera of sheep infected withEchinococcus granulosus, Taenia hydatigena andTaenia ovis. Parasite Immunol 1:27–38
Yong WK, Heath DD, Parmeter SN (1978)Echinococcus granulosus, Taenia hydatigena, T. ovis: evaluation of cyst fluids as antigen for serodiagnosis of larval cestodes in sheep. NZ Vet J 26:231–234
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Liu, D., Rickard, M.D. & Lightowlers, M.W. Analysis of taeniid antigens using monoclonal antibodies toEchinococcus granulosus antigen 5 and antigen B. Parasitol Res 79, 82–85 (1993). https://doi.org/10.1007/BF00931225
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00931225