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Trypanosoma cruzi: the effect of variations in experimental conditions on the levels of macrophage infection in vitro

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Abstract

A comparative and systematic analysis of the different experimental conditions used inTrypanosoma cruzi-macrophage interaction assays is presented. A pH range from 6.2 to 6.9 is favorable for parasite invasion, as is the use of Dulbecco's Modified Eagle Medium (DMEM). The washing procedures used in purification protocols also enhance the parasites' ability to penetrate macrophages. However, the main factor affecting the quantitative parameter of this in vitro infection, regardless of the parasite: cell ratio, is the number of macrophages provided to the parasites. These results, reviewed and compared with the corresponding literature, strongly suggest that macrophage surface areas and/or receptors are the limiting factors for optimal quantitative determination of the interaction ofT. cruzi in vitro.

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Authors and Affiliations

  1. Departamento de Ultraestrutura e Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Manguinhos, 21040, Rio de Janeiro, RJ, Brasil

    Tania C. Araújo-Jorge, Elizabeth P. Sampaio & Maria de Nazareth Leal de Meirelles

  2. Laboratório de Ultraestrutura Celular, Instituto de Biofisica Carlos Chagas Filho, CCS-UFRJ, Cidade Universitária, 21941, Rio de Janeiro, RJ, Brasil

    Wanderley De Souza

Authors
  1. Tania C. Araújo-Jorge
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  2. Elizabeth P. Sampaio
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  3. Wanderley De Souza
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  4. Maria de Nazareth Leal de Meirelles
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Araújo-Jorge, T.C., Sampaio, E.P., De Souza, W. et al. Trypanosoma cruzi: the effect of variations in experimental conditions on the levels of macrophage infection in vitro. Parasitol Res 75, 257–263 (1989). https://doi.org/10.1007/BF00931809

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