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Determination of the substitution patterns of cellulose methyl ethers by HPLC and GLC-comparison of methods

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Summary

The substitution patterns of methyl cellulose as well as of a thexyldimethylsilyl cellulose after permethylation were determined by hydrolysis and separation of the resulting partially methylated glucoses without further derivatization by h.p.l.c.. On an amine-modified silica column the solutes get separated into glucose, 2,3,6-tri-O-methyl glucose and the groups of mono-O-methyl-and di-O-methyl glucoses. A chromatographic run on a reversed-phase column enables the identification of the single mono-O-methyl- and di-O-methyl glucoses. In this way, a determination of both the average degree of substitution and the substitution pattern of cellulose derivatives is possible. Comparison of the results with those obtained by standard methylation analysis including g.l.c.-m.s. proves the correctnes of the method employed.

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Authors and Affiliations

  1. Institut für Organische Chemie und Makromolekulare Chemie, Universität, Humboldtstrasse 10, O-6900, Jena, Federal Republic of Germany

    U. Erler, A. Stein & D. Klemm

  2. Institut für Organische Chemie, Universität, Martin-Luther-King-Platz 6, W-200, Hamburg 13, Federal Republic of Germany

    P. Mischnick

Authors
  1. U. Erler
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  2. P. Mischnick
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  3. A. Stein
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  4. D. Klemm
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Erler, U., Mischnick, P., Stein, A. et al. Determination of the substitution patterns of cellulose methyl ethers by HPLC and GLC-comparison of methods. Polymer Bulletin 29, 349–356 (1992). https://doi.org/10.1007/BF00944830

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  • DOI: https://doi.org/10.1007/BF00944830

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