Abstract
Expression sites of genes encoding (1→3,1→4)-β-glucan 4-glucanohydrolase (EC 3.2.1.73) have been mapped in germinated barley grains (Hordeum vulgare L.) by hybridization histochemistry. A32P-labelled cDNA (copy DNA) probe was hybridized to cryosections of intact barley grains to localize complementary mRNAs. No mRNA encoding (1→3,1→4)-β-glucanase is detected in ungerminated grain. Expression of (1→3,1→4)-β-glucanase genes is first detected in the scutellum after 1 d and is confined to the epithelial layer. At this stage, no expression is apparent in the aleurone. After 2 d, levels of (1→3,1→4)-β-glucanase mRNA decrease in the scutellar epithelium but increase in the aleurone. In the aleurone layer, induction of (1→3,1→4)-β-glucanase gene expression, as measured by mRNA accumulation, progresses from the proximal to distal end of the grain as a front moving away from, and parallel to, the face of the scutellum.
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Abbreviations
- cDNA:
-
copy DNA
- RNase:
-
ribonuclease
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McFadden, G.I., Ahluwalia, B., Clarke, A.E. et al. Expression sites and developmental regulation of genes encoding (1→3,1→4)-β-glucanases in germinated barley. Planta 173, 500–508 (1988). https://doi.org/10.1007/BF00958963
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DOI: https://doi.org/10.1007/BF00958963