Abstract
The abscisic-acid (ABA) contents of isolated guard-cell protoplasts and mesophyll-cell protoplasts fromVicia faba were determined by high-pressure liquid chromatography followed by gas chromatography. The amounts of ABA found immediately after preparation of the protoplasts varied from 90 to 570 amol per guard-cell protoplast, and from 75 to 100 amol per mesophyll-cell protoplast. These contents correspond to concentrations between 36 and 230 μmol per liter in guard-cell protoplasts and between 2.7 and 3.3 μmol per liter in mesophyll-cell protoplasts. During exposure of protoplasts to betaine concentrations of 0.3, 0.5, and 0.8 mol·l-1 at 0° and 20°C for 30 min, ABA contents as well as the fractions of ABA that leaked into the medium remained constant for both protoplast types. There was no evidence for net production of ABA in isolated protoplasts subjected to osmotic stress.
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Abbreviations
- ABA:
-
abscisic acid
References
Behl, R., Hartung, W. (1986) Movement and compartmentation of absicic acid in guard cells ofValerianella locusta: Effects of osmotic stress, external H+-concentration and fusicoccin. Planta168, 360–368
Bray, E., Zeevaart, J.A.D. (1985) The compartmentation of abscisic acid andβ-d-glucopyranosil abscisate in mesophyll cells. Plant Physiol.79, 719–722
Cornish, K., Zeevaart, J.A.D. (1985) Movement of abscisic acid into the apoplast in response to water stress inXanthium strumarium. Plant Physiol.78, 623–626
Cornish, K., Zeevaart, J.A.D. (1986) Abscisic acid accumulation by in situ and isolated guard cells ofPisum sativum L. andVicia faba L. in relation to water stress. Plant Physiol.77, 25–28
Creelman, R.A., Zeevaart, J.A.D. (1985) Abscisic acid accumulation in spinach leaf slices in the presence of penetrating and non-penetrating solutes. Plant Physiol.75, 166–169
Driessche, E., van, Beeckmans, S., Dejaegere, R., Kanarek, L. (1984) Thiourea: the antioxidant of choice for the purification of protein from phenolrich plant tissue. Anal. Biochem.141, 184–188
Gotow, K., Shimazaki, K., Kondo, N., Syono, K. (1984) Photosynthesis-dependent volume regulation in guard-cell protoplasts fromV. faba L. Plant Cell Physiol.25, 671–675
Grantz, D.A., Ho, T.-H.D., Uknes, S.J., Cheeseman, J.M., Boyer, J.S. (1985) Metabolism of abscisic acid in guard cells ofVicia faba L. andCommelina communis L. Plant Physiol.78, 51–56
Hedrich, R., Raschke, K., Stitt, M. (1985) A role for fructose 2,6-bisphosphate in regulating carbohydrate metabolism in guard cells. Plant Physiol.79, 977–982
Loveys, B.R., Robinson, S.P. (1987) Abscisic acid synthesis and metabolism in barley leaves and protoplasts. Plant Sci. Lett.49, 23–30
Pierce, M.L., Raschke, K. (1980) Correlation between loss of turgor and accumulation of abscisic acid in detached leaves. Planta148, 174–182
Schnabl, H. (1980) CO2 and malate metabolism in starch-containing and starch-lacking guard-cell protoplasts. Planta149, 52–58
Shimazaki, K., Gotow, K., Kondo, N. (1982) Photosynthetic properties of guard-cell protoplasts fromVicia faba L. Plant Cell Physiol.23, 871–879
Weiler, E.W., Schnabl, H., Hornberg, C. (1982) Stress-related levels of abscisic acid in guard-cell protoplasts ofVicia faba L. Planta154, 24–28
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Lahr, W., Raschke, K. Abscisic-acid contents and concentrations in protoplasts from guard cells and mesophyll cells ofVicia faba L.. Planta 173, 528–531 (1988). https://doi.org/10.1007/BF00958966
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DOI: https://doi.org/10.1007/BF00958966