Abstract
The main glycerolipids (monogalactosyl-, digalactosyl-, sulphoquinovosyl diacylglycerol, phosphatidylglycerol) from five blue-green algae (Microcystis, Anabaena, Nostoc, Oscillatoria, Tolypothrix) were analyzed for fatty acid composition, occurrence of diglyceride species and positional distribution of fatty acids between thesn-1- andsn-2-position of glycerol. In contrast to eucaryotic plants biosynthetically closely related lipids (monogalactosyl-, digalactosyl-, trigalactosyl diacylglycerol) show nearly identical diglyceride moieties, whereas sulphoquinovosyl diacylglycerol and phosphatidylglycerol are separated from galactolipids by composition as well as occurrence of fatty acids. On the other hand the positional distribution of fatty acids in all lipids is controlled exclusively by chain length and not by degree of unsaturation with C18-fatty acids at thesn-1- and C16-fatty acids at thesn-2-position. These results show that in procaryotic organisms the diversity in diglyceride portions of lipids is reduced as compared to eucaryotic organisms, but nevertheless does exist.
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Abbreviations
- MGD, DGD, TGD, SQD:
-
monogalactosyl-, digalactosyl-, trigalactosyl-, sulphoquinovosyl diacylglycerol
- PG:
-
phosphatidyl glycerol
References
Allen, M. B., Arnon, D. J.: Studies on nitrogen-fixing blue-green algae. I. Growth and nitrogen fixation byAnabaena cylindrica Lemm. Plant Physiol.30, 366–372 (1955)
Budzikiewicz, H., Rullkötter, J., Heinz, E.: Massenspektroskopische Untersuchungen an Glycosylglyceriden. Z. Naturforsch.28c, 499–504 (1973)
Douce, R., Benson, A. A.: Isolation, purification and properties of the envelope of spinach chloroplasts. Portug. Acta Biol.14, 45–64 (1974)
Fischer, W.: The polar lipids of group B streptococci. II. Composition and positional distribution of fatty acids. Biochim. Biophys. Acta487, 89–104 (1977)
Gent, P. A., Gigg, R.: Synthesis of 3-O-(6-O-[6-O-α-D-galactopyranosyl-α-D-galactopyranosyl]-β-D-galactopyranosyl)-1,2-di-O-stearoyl-L-glycerol, a ‘trigalactosyl diglyeride’. J. Chem. Soc. Perk. Trans.1, 1779–1781 (1975)
Heinz, E.: Über die enzymatische Bildung von Acylgalaktosyldiglycerid. Biochim. Biophys. Acta144, 333–343 (1967)
Heinz, E.: Enzymatic reactions in galactolipid biosynthesis. In: Lipids and lipid polymers in higher plants (M. Tevini, H. K. Lichtenthaler, eds.), pp. 102–115. Berlin-Heidelberg-New York: Springer 1977
Hirayama, O.: Lipids and lipoprotein complex in photosynthetic tissues. II. Pigments and lipids in blue-green alga,Anacystis nidulans. J. Biochem.61, 179–185 (1967)
Hitchcock, C., Nichols, B. W.: Plant lipid biochemistry. London-New York: Academic Press 1971
Kagawa, Y., Ariga, T.: Determination of molecular species of phospholipids from thermophilic bacterium PS 3 by mass chromatography. J. Biochem.81, 1161–1165 (1977)
Kenyon, C. N.: Fatty acid composition of unicellular strains of blue-green algae. J. Bacteriol.109, 827–834 (1972)
Kenyon, C. N., Rippka, R., Stanier, R. Y.: Fatty acid composition and physiological properties of some filamentous blue-green algae. Arch. Mikrobiol.83, 216–236 (1972)
Kenyon, C. N., Stanier, R. Y.: Possible evolutionary significance of polyunsaturated fatty acids in blue-green algae. Nature227, 1164–1166 (1970)
Koch, W.: Verzeichnis der Sammlung von Algenkulturen am Pflanzenphysiologischen Institut der Universität Göttingen. Arch. Mikrobiol.47, 402–432 (1964)
Lambein, F., Wolk, C. P.: Structural studies on the glycolipids from the envelope of the heterocyst ofAnabaena cylindrica. Biochemistry12, 791–798 (1973)
Lang, N. J., Whitton, B. A.: Arrangement and structure of thylakoids. In: The biology of blue-green algae (N. G. Carr, B. A. Whitton, eds.), pp. 66–79. Berkeley-Los Angeles: University of California Press 1973
Mereschkowsky, C.: Über Natur und Ursprung der Chromatophoren im Pflanzenreiche. Biol. Zentralbl.25, 593–604 (1905)
Nakano, M., Fischer, W.: The glycolipids ofLactobacillus casei DSM 20021. Hoppe-Seyler's Z. Physiol. Chem.358, 1439–1453 (1977)
Nichols, B. W.: Comparative lipid biochemistry of photosynthetic organisms. In: Phytochemical phylogeny (J. B. Harbone, ed.), pp. 105–118. London-New York: Academic Press 1970
Nichols, B. W.: Lipid composition and metabolism. In: The biology of blue-green algae (N. G. Carr, B. A. Whitton, eds.), pp. 144–161. Berkeley-Los Angeles: University of California Press 1973
Nichols, B. W., Wood, B. J. B.: The occurrence and biosynthesis of gamma-linolenic acid in a blue-green alga,Spirulina platensis. Lipids3, 46–50 (1968)
Radunz, A.: Über das Sulfochinovosyl-diacylglycerin aus höheren Pflanzen, Algen und Purpurbakterien. Hoppe-Seyler's Z. Physiol. Chem.350, 411–417 (1969)
Radunz, A.: Localisation of the tri- and digalactosyl diglyceride in the thylakoid membrane with serological methods. Z. Naturforsch.31c, 589–593 (1976)
Rullkötter, J., Heinz, E., Tulloch, A. P.: Combination and positional distribution of fatty acids in plant digalactosyl diglycerides. Z. Pflanzenphysiol.76, 163–175 (1975)
Safford, R., Nichols, B. W.: Positional distribution of fatty acids in monogalactosyl diglyceride fractions from leaves and algae. Structural and metabolic studies. Biochim. Biophys. Acta210, 57–64 (1970)
Schmitz, R.: Über die Zusammensetzung der pigmenthaltigen Strukturen aus Prokaryonten. I. Untersuchungen an Thylakoiden vonOscillatoria chalybea Kütz. Arch. Mikrobiol.56, 225–237 (1967)
Shivley, J. M.: Inclusion bodies of procaryotes. Ann. Rev. Microbiol.28, 167–187 (1974)
Siebertz, H. P., Heinz, E.: Labelling experiments on the origin of hexa- and octadecatrienoic acids in galactolipids from leaves. Z. Naturforsch.32c, 193–205 (1977)
Tuquet, C., Guillot-Salomon, T., de Lubac, M., Signol, M.: Granum formation and the presence of phosphatidylglycerol containing trans-Δ 3-hexadecenoic acid. Plant Sci. Lett.8, 59–64 (1977)
Veerkamp, J. H.: Biochemical changes inBifidobacterium bifidum var.pennsylvanicus after cell wall inhibition. V. Structure of the galactosyldiglycerides. Biochim. Biophys. Acta273, 359–367 (1972)
Wood, B. J. B.: Fatty acids and saponifiable lipids. In: Algal physiology and biochemistry (W. D. P. Stewart, ed.), pp. 236–265. Oxford-London-Edinburgh-Melbourne: Blackwell 1974
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Zepke, H.D., Heinz, E., Radunz, A. et al. Combination and positional distribution of fatty acids in lipids from blue-green algae. Arch. Microbiol. 119, 157–162 (1978). https://doi.org/10.1007/BF00964267
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DOI: https://doi.org/10.1007/BF00964267