Skip to main content
SpringerLink
Log in

Partial characterization of an endogenous factor which modulates the effect of catecholamines on synaptosomal Na+, K+-ATPase

  • Original Articles
  • Published:
Neurochemical Research Aims and scope Submit manuscript

Abstract

We have previously presented evidence for the existence of a brain soluble factor which mediates the stimulation of synaptosomal ATPases by catecholamines. The stimulation of synaptosomal ATPases by dopamine plus brain soluble fraction was not modified if the soluble fraction was heated for 5 min at 95°C. One day after preparation, the soluble factor inhibited the Na+, K+-ATPase, but not the Mg2+-ATPase activity, and subsequent addition of noradrenaline stimulated the ATPases activities. The inhibitory effect of a 24 h soluble fraction disappeared if the soluble fraction was dialyzed; in this case, noradrenaline did not activate the enzyme activities. Gel filtration in Sephadex G-50 permitted separating a subfraction which inhibited ATPase activity (peak II) from another which stimulated ATPase activity (peak I). Peak I stimulated both Na+, K+, and Mg2+ ATPases. Peak II inhibited only Na+, K+-ATPase, and when stored acidified, it mediated ATPases stimulation by noradrenaline.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Albers, R., Rodríguez de Lores Arnaiz, G., andDe Robertis, E. 1965. Sodium-potassium-activated ATPase and potassium-activated p-nitrophenyl-phosphatase: a comparison of their subcellular localizations in rat brain. Proc. Natl. Acad. Sci. USA 53:557–564.

    Google Scholar 

  2. Rodríguez de Lores Arnaiz, G. Alberici, M., andDe Robertis, E. 1967. Ultrastructural and enzymic studies of cholinergic and non-cholinergic synaptic membranes isolated from brain cortex. J. Neurochem. 14:215–225.

    Google Scholar 

  3. Schaefer, A., Unyi, G., andPfeifer, A. K. 1972. The effects of a soluble factor and of catecholamines on the activity of adenosine triphosphatase in subcellular fractions of rat brain. Biochem. Pharmacol. 21:2289–2294.

    Google Scholar 

  4. Yoshimura, K. 1973. Activation of Na−K activated ATPase in rat brain by catecholamine. J. Biochem. 74:389–391.

    Google Scholar 

  5. Gilbert, J. C., Wyllie, M. G., andDavison, D. V. 1975. Nerve terminal ATPase as possible trigger for neurotransmitter release. Nature 255:237–238.

    Google Scholar 

  6. Logan, J. G., andO'Donovan, D. J. O. 1976. The effects of ouabain and the activation of neural membrane ATPase by biogenic amines. J. Neurochem. 27:185–189.

    Google Scholar 

  7. Desaiah, D., andHo, T. K. 1977. Kinetics of catecholamines sensitive Na+, K+-ATPase activity in mouse brain synaptosomes. Biochem. Pharmac. 26:2029–2035.

    Google Scholar 

  8. Lee, S. L., andPhillis, J. W. 1977. Stimulation of cerebral cortical synaptosomal Na+ K+-ATPase by biogenic amines. Can. J. Physiol. Pharmac. 55:961–964.

    Google Scholar 

  9. Rodríguez de Lores Arnaiz, G., andMistrorigo de Pacheco, M. 1978. Regulation of Na+ K+ adenosinetriphosphatase on nerve ending membranes. Action of norepinephrine and a soluble factor. Neurochem. Res. 3:733–744.

    Google Scholar 

  10. Rodríguez de Lores Arnaiz, G., andAntonelli de Gómez de Lima, M. 1981. The effect of several neurotransmitter substances on nerve ending membrane ATPase. Acta Physiol. Latinoam. 31:39–44.

    Google Scholar 

  11. Antonelli de Gómez de Lima, M., andRodríguez de Lores Arnaiz, G. 1981. Tissue specificity of dopamine effects on brain ATPases. Neurochem. Res. 6:969–977.

    Google Scholar 

  12. Rodríguez de Lores Arnaiz, G., andAntonelli de Gómez de Lima, M. 1983. The effects of dopamine on Na+ K+-ATPase activity in nerve ending membranes are prevented by N-ethyl-maleimide. Neurochem. Int. 5:117–124.

    Google Scholar 

  13. Lowry, O. H., andLópez, J. A. 1946. Determination of inorganic phosphate in presence of labile P esters. J. Biol. Chem. 162:421–428.

    Google Scholar 

  14. Lowry, O. H., Rosebrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.

    Google Scholar 

  15. Rodríguez de Lores Arnaiz, G. andDe Robertis, E. 1972. Properties of the isolated nerve endings. Pages 237–272,in Bronner, F., andKleinzeller, A. (eds.), Current Topics in Membranes and Transport, Vol. 3, Academic Press, New York.

    Google Scholar 

  16. Underwood, E. J. 1977. In Trace elements in human and animal nutrition. (4th Ed.) Pages 388–397, Academic Press, London.

    Google Scholar 

  17. Josephson, L., andCantley, L. C. Jr. 1977. Isolation of a potent (Na+−K+) ATPase inhibitor from striated muscle. Biochemistry 16:4572–4578.

    Google Scholar 

  18. Cheung, W. Y. 1980. Calmodulin plays a pivotal role in cellular regulation. Science 207:19–27.

    Google Scholar 

  19. Swanson, P. D., andStahl, W. L. 1976. Ion transport. Pages 125–147,in Siegel, G., Albers, R. W., Katzman, K., andAgranoff, B. (eds.), Basic Neurochemistry, Little Brown and Co., Boston, 2nd Ed.

    Google Scholar 

  20. Fishman, M. C. 1979. Endogenous digitalis-like activity in mammalian brain. Proc. Natl. Acad. Sci. U.S.A. 76:4661–4663.

    Google Scholar 

  21. Haupert, G. T., andSancho, J. M. 1979. Sodium transport inhibitor from bovine hypothalamus. Proc. Natl. Acad. Sci. U.S.A. 76:4658–4660.

    Google Scholar 

  22. Lichtstein, D., andSamuelov, S. 1980. Endogenous ‘ouabain like’ activity in brain. Biochem. Biophys. Res. Comm. 96:1518–1523.

    Google Scholar 

  23. Akagawa, K., Hara, N., andTsukada, Y. 1984. Partial purification and properties of the inhibitors of Na+ K+-ATPase and ouabain-binding in bovine central nervous system. J. Neurochem. 42:775–780.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto de Biología Celular Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155-(1121), Buenos Aires, Argentina

    G. Rodríguez de Lores Arnaiz & M. Antonelli de Gómez de Lima

Authors
  1. G. Rodríguez de Lores Arnaiz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Antonelli de Gómez de Lima
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Special Issue dedicated to Prof. Eduardo De Robertis.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rodríguez de Lores Arnaiz, G., Antonelli de Gómez de Lima, M. Partial characterization of an endogenous factor which modulates the effect of catecholamines on synaptosomal Na+, K+-ATPase. Neurochem Res 11, 933–947 (1986). https://doi.org/10.1007/BF00965584

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00965584

Keywords

Search

Navigation