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Age does not alter protein kinase C isozymes mRNA expression in rat brain

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Abstract

Calcium and phospholipid dependent Protein kinase C (PKC) may play a role in memory function and pathogenesis of many neurodegenerative disorders such as Alzheimer's disease (AD). Abnormal phosphorylation by PKC as well as reduced levels of PKC has been implicated in the neurodegeneration associated with AD and aging. Recently, many subtypes of PKC isozymes have been identified by molecular biology techniques which are expressed differentially in various regions of the brain. The reduction and alterations in the activities and distribution of these subtypes of PKC isozymes may be accountable for the decline of selective neurons during aging. In order to investigate the role of PKC isozymes during aging, we examined the distribution of PKC-α, β, and γ mRNA, expressions between young (4 months) and old (25 months) rat brains using in situ hybridization histochemistry. Our studies showed that signals of three isoforms of PKC mRNA vary in cortical and hippocampal regions. However, no change was detected in any of the PKC isoforms mRNA expressions in aged animals.

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Authors and Affiliations

  1. Neuropsychiatric Research Institute, 700 South First Avenue, Fargo, North Dakota

    Neelam Narang

  2. Center for Alcohol Research, University of North Carolina, College of Medicine, Chapel Hill, North Carolina

    Fulton T. Crews

  3. Departments of Neuroscience and Pharmacology, University of North Dakota, Grand Forks, North Dakota

    Neelam Narang

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  1. Neelam Narang
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  2. Fulton T. Crews
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Narang, N., Crews, F.T. Age does not alter protein kinase C isozymes mRNA expression in rat brain. Neurochem Res 20, 1119–1126 (1995). https://doi.org/10.1007/BF00995373

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