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Changes in erythrocyte 2,3 diphosphoglycerate in women following short term maximal exercise

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Abstract

15 untrained women were subjected to a walking treadmill test to determine the influence of maximal exercise upon synthesis of erythrocyte 2,3 DPG. Although there was a 9.8% increase in the 2,3 DPG content following exercise, there was a concomitant 9.4% increase in the hemoglobin level; therefore, when 2,3 DPG is expressed as a ratio to hemoglobin\(\frac{{(DPG\mu M/ml)}}{{(Hb gm/\% )}}\) there was no significant change as a result of exercise stress. It was suggested that three additive factors produced during strenuous exercise: decreased pH; increased hemoglobin concentration; and increased CO2 production result in by-product inhibition of 2,3 DPG synthesis. It is concluded that 2,3 DPG does not provide a physiologic benefit in the adaptation of the oxygen transport system to exercise.

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References

  1. Astrup, P., Engle, K., Severinghaus, J. W., Munson, E.: The influence of temperature and pH on the dissociation curve of oxygen-hemoglobin of human blood. Scand. J. clin. Lab. Invest.17, 515–523 (1965)

    Google Scholar 

  2. Austin, P. L., Stegink, L. D., Gisolfi, C. V., Lauer, R. M.: The effect of exercise on red blood cell 2,3-diphosphoglycerate in children. Pediatrics83, 41–45 (1973)

    Google Scholar 

  3. Bellingham, A. J., Detter, J. C., Lenfant, C.: Regulatory mechanisms of hemoglobin oxygen affinity in acidosis and alkalosis. J. clin. Invest.50, 700–706 (1971)

    Google Scholar 

  4. Benesch, R., Benesch, R. E.: Intracellular organic phosphates as regulators of oxygen release by hemoglobin. Nature (Lond.)221, 618–622 (1969a)

    Google Scholar 

  5. Benesch, R. E., Benesch, R., Ing Yu, C.: The oxygenation of hemoglobin in the presence of 2,3-diphosphoglycerate: Effect of temperature, pH, ionic strength and hemoglobin concentration. Biochemistry8, 2567–2571 (1969b)

    Google Scholar 

  6. Benesch, R. E., Benesch, R.: The reaction between diphosphoglycerate and hemoglobin. Fed. Proc.29, 1101–1104 (1970)

    Google Scholar 

  7. Brewer, G. J., Eaton, J. W.: Erythrocyte metabolism: Interaction with oxygen transport. Science171, 1205–1211 (1971)

    Google Scholar 

  8. Brewer, G. J.: 2,3-DPG and erythrocyte oxygen affinity. Ann. Rev. Med.25, 29–38 (1974)

    Google Scholar 

  9. Brewer, G. J., Oelshlegel, F. J., Jr., Schoomaker, E. B., Knutsen, C. A.: Potential effects of hemoglobin concentration and red cell metabolism together with observations on red cell metabolic differences between men and women. Advanc. exp. Med. Biol.28, 99–119 (1972)

    Google Scholar 

  10. Dempsey, J. A., Rodriquez, J., Shahidi, N. T., Reddan, W. G., MacDougall, J. D.: Muscular exercise, 2,3-DPG and oxy-hemoglobin affinity. Int. Z. angew. Physiol.30, 34–39 (1971)

    Google Scholar 

  11. Doll, E., Keul, J., Maiwald, C.: Oxygen tension and acid-base equilibria in venous blood of working muscle. Amer. J. Physiol.215, 23–29 (1968)

    Google Scholar 

  12. Eaton, J. W., Brewer, G. J.: The relationship between red cell 2,3-diphosphoglycerate and levels of hemoglobin in the human. Proc. nat. Acad. Sci. (Wash.)61, 756–760 (1968)

    Google Scholar 

  13. Faulkner, J. A., Brewer, G. J., Eaton, J. W.: Adaptation of the red blood cell to muscular exercise. Advanc. exp. Med. Biol.6, 213–227 (1970)

    Google Scholar 

  14. Eaton, J. W., Brewer, G. J., Schultz, J. S., Sing, C. F.: Variation in 2,3-diphosphoglycerate and ATP levels in human erythrocytes and effects on oxygen transport. Advanc. exp. Med. Biol.28, 21–38 (1972)

    Google Scholar 

  15. Eaton, J. W., Faulkner, J. A., Brewer, G. J.: Response of the human red cell to muscular activity. Proc. Soc. exp. Biol. (N.Y.)132, 886–887 (1969)

    Google Scholar 

  16. Garby, L., de, Verdier C. H.: Binding of 2,3 diphosphoglycerate to human hemoglobin A — Effects of pH, hemoglobin concentration, and carbon dioxide. In: Oxygen affinity of hemoglobin and red cell acid base status, P. Astrup, M. Rørth, eds., pp. 236–242. Copenhagen: Alfred Benzon Foundation 1972

    Google Scholar 

  17. Gerlach, E., Duhm, J., Deuticke, B.: Metabolism of 2,3-diphosphoglycerate in red blood cells under various experimental conditions. Advanc. exp. Med. Biol.6, 155–174 (1970)

    Google Scholar 

  18. Holmgren, A., McIlroy, M. B.: Effect of temperature on arterial blood gas tension and pH during exercise. J. appl. Physiol.19, 243–245 (1964)

    Google Scholar 

  19. Klocke, R. A.: Oxygen transport and 2,3 diphosphoglycerate (DPG). Chest62, 625–631 (1971)

    Google Scholar 

  20. Lenfant, C., Torrance, J., English, E., Finch, C. A., Reynafarje, C., Ramos, J., Faura, J.: Effect of altitude on oxygen binding by hemoglobin and on organic phosphate levels. J. clin. Invest.47, 2652–2656 (1968)

    Google Scholar 

  21. Lenfant, C., Torrance, J. D., Reynafarje, C.: Shift of the O2-Hb dissociation curve at altitude: mechanism and effect. J. appl. Physiol.30, 625–631 (1971)

    Google Scholar 

  22. Magazanik, A., Shapiro, Y., Meytes, D., Meytes, I.: Enzyme blood levels and water balance during a marathon race. J. appl. Physiol.36, 214–217 (1974)

    Google Scholar 

  23. McArdle, W. D., Katch, F. I., Pechar, G. S., Jacobson, L., Ruch, S.: Reliability and interrelationships between maximal oxygen intake, physical work capacity and step-test scores in college women. Med. Sci. Sports4, 182–186 (1972)

    Google Scholar 

  24. Rørth, M., Nygaard, S. F.: Phosphate metabolism of the red cell during exposure to high altitude (4,500 m). In: Oxygen affinity of hemoglobin and red cell acid base status, P. Astrup, M. Rørth, eds., pp. 599–608. Copenhagen: Alfred Benzon Foundation 1972a

    Google Scholar 

  25. Rørth, M., Nygaard, S. F., Parving, H. H.: Effect of exposure to simulated high altitude on human red blood phosphates and oxygen affinity of hemoglobin. Influence of exercise. Scand. J. clin. Lab. Invest.29, 329–333 (1972b)

    Google Scholar 

  26. Rose, Z. B., Liebowitz, J.: 2,3-Diphosphoglycerate phosphotase from human erythrocytes. J. biol. Chem.245, 3232–3241 (1970)

    Google Scholar 

  27. Shappell, S. D., Murray, J. A., Bellingham, A. J., Woodson, R. D., Detter, J. C., Lenfant, C.: Adaptation to exercise: Role of hemoglobin affinity for oxygen and 2,3-diphospho-glycerate. J. appl. Physiol.30, 827–832 (1971)

    Google Scholar 

  28. Sloan, A. W., Burt, J. J., Blyth, C. S.: Estimation of body fat in young women. J. appl. Physiol.17, 967–970 (1962)

    Google Scholar 

  29. Taunton, J. E., Taunton, C. A., Banister, E. W.: Alterations in 2,3-DPG and P50 with maximal and submaximal exercise. Med. Sci. Sports6, 238–241 (1974)

    Google Scholar 

  30. Thomson, J. M., Dempsey, J. A., Chosy, L. W., Shahidi, N. T., Reddan, W. G.: Oxygen transport and oxyhemoglobin dissociation during prolonged muscular work. J. appl. Physiol.37, 658–664 (1974)

    Google Scholar 

  31. Tomita, S., Riggs, A.: Studies of the interaction of 2,3-diphosphoglycerate from mouse, man, and elephant. J. biol. Chem.246, 547–554 (1971)

    Google Scholar 

  32. Van Beaumont, W., Greenleaf, J. E., Juhos, L.: Disproportional changes in hematocrit, plasma volume, and proteins during exercise and bed rest. J. appl. Physiol.33, 55–61 (1972)

    Google Scholar 

  33. Wilmore, J. H., Norton, A. C.: The heart and lungs at work, p. 23. Shiller Park: Beckman Instruments 1974

    Google Scholar 

  34. Woodson, R. B., Wranne, B., Better, J.: Effect of erythrocyte 2,3 DPG and hemoglobin concentration on O2 delivery and work performance. Clin. Res.20, 628 (1972)

    Google Scholar 

  35. Wranne, B., Nordgren, L., Woodson, R. D.: Increased blood oxygen affinity and physical work capacity in man. Scand. J. clin. Lab. Invest.33, 347–352 (1974)

    Google Scholar 

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Authors and Affiliations

  1. Department of Health, Physical Education, and Recreation, University of Texas, 78712, Austin, Texas

    H. W. Bonner, C. A. Tate & C. K. Buffington

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  1. H. W. Bonner
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  2. C. A. Tate
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Bonner, H.W., Tate, C.A. & Buffington, C.K. Changes in erythrocyte 2,3 diphosphoglycerate in women following short term maximal exercise. Europ. J. Appl. Physiol. 34, 227–232 (1975). https://doi.org/10.1007/BF00999936

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  • DOI: https://doi.org/10.1007/BF00999936

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