Summary
A simple method for fixing and embedding plant tissue for hybridization histochemistry has been developed. Material is fixed with paraformaldehyde-glutaraldehyde by the phase-partition technique and embedded in LR Gold. The method preserves nucleic acidsin situ. Double-stranded32P-labelled DNA probes hybridized to complementary RNAs in thin and semithin sections. Biotinylated probes detected with immunogold markers have been used to localize transcripts at the electron nucroscopic level.
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McFadden, G.I., Bonig, I., Cornish, E.C. et al. A simple fixation and embedding method for use in hybridization histochemistry on plant tissues. Histochem J 20, 575–586 (1988). https://doi.org/10.1007/BF01002612
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DOI: https://doi.org/10.1007/BF01002612