Summary
Chemical and histochemical methods were used to compare the epithelial glycoproteins from formalin-fixed surgical specimens of normal human large intestine, colonic tumours, ulcerative colitis and diverticular disease. All the epithelial glycoproteins contained fucose, galactose, glucosamine, galactosamine and, in addition, sialic acids both with and withoutO-acyl substituents in the side chain and/or at position C4. The glycoproteins of the normal ascending and descending colons differed significantly with respect to the percentage of the sialic acids released following digestion of the de-O-acylated glycoprotein withVibrio cholera neuraminidase and to the molar fucose-sialic acid ratio. Statistical analysis of the chemical data showed that (a) compared to normal, the sialic acids of the tumour and ulcerative colitis glycoproteins from the descending colon were significantly less substituted in the side chain and at position C4; (b) theO-acetyl substitution pattern of the sialic acids of the ulcerative colitis glycoproteins from the ascending colon and the quantitative composition of the carbohydrate prosthetic groups of the ulcerative colitis glycoproteins from both ascending and descending colons differed from normal; (c) it was not always possible to distinguish between the ulcerative colitis and tumour glycoproteins on the basis of theO-acetyl substitution pattern of their sialic acids; and (d), there were minor differences between normal glycoproteins and those from cases of diverticular disease.
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References
Culling, C. F. A. (1974)Handbook of Histopathological and Histochemical Technique. 3rd edn. London: Butterworth.
Culling, C. F. A. &Reid, P. E. (1980) Specific techniques for the identification ofO-acylated sialic acids in colonic mucins.J. Microscopie 119, 415–25.
Culling, C. F. A., Reid, P. E., Burton, J. D., &Dunn, W. L. (1975) A histochemical method of differentiating lower gastrointestinal tract mucin from other mucins in primary or metastatic tumours.J. Clin. Pathol. 28, 656–8.
Culling, C. F. A., Reid, P. E. &Dunn, W. L. (1971) The effect of saponification upon certain histochemical reactions of the epithelial mucins of the G.I. tract.J. Histochem. Cytochem. 19, 654–62.
Culling, C. F. A., Reid, P. E. &Dunn, W. L. (1976). A new histochemical method for the identification and visualization of both side-chain acylated and non-acylated sialic acids.J. Histochem. Cytochem. 24, 1225–30.
Culling, C. F. A., Reid, P. E. &Dunn, W. L. (1977b) A histochemical comparison of the epithelial mucin of the ileum in Crohn's disease and in normal controls.J. clin. Path. 31, 1063–7.
Culling, C. F. A., Reid, P. E. &Dunn, W. L. (1979) A histochemical comparison of theO-acetylated sialic acids of the epithelial mucins in ulcerative colitis Crohn's disease and normal controls.J. clin. Path. 32, 1272–7.
Culling, C. F. A., Reid, P. E., Dunn, W. L. &Clay, M. G. (1974) The histochemical demonstration ofO-acylated sialic acids in gastrointestinal mucins: Their association with the potassium hydroxide-periodic acid-Schiff effect.J. Histochem. Cytochem. 22, 826–31.
Culling, C. F. A., Reid, P. E., Dunn, W. L. &Freeman, H. J. (1981b) The relevance of the histochemistry of colonic mucins based upon their PAS reactivity.Histochem. J. 13, 889–903.
Culling, C. F. A., Reid, P. E., Ramey, C. W., Dunn, W. L. &Clay, M. G. (1977c) A method for the simultaneous estimation of free and ketosidically bound sialic acid.Can. J. Biochem. 55, 778–82.
Culling, C. F. A., Reid, P. E. &Worth, A. J. (1981a) Carbohydrate markers in the lower gastro-intestinal tract. InMethods and Achievements in Experimental Pathology; Cell Markers (edited byJasmin, G. andCantin, M.) vol. 10, pp. 77–100. Basel: S. Karger.
Culling, C. F. A., Reid, P. E., Worth, A. J. &Dunn, W. L. (1977a) A new histochemical technique of use in the interpretation and diagnosis of adenocarcinomas and villous lesions in the large intestine.J. clin. Path. 30, 1056–62.
Dawson, I. M. P. (1972) The histochemistry of Crohn's disease.Clin. Gastroenterol. 1, 309–20.
Dawson, P. A., Patel, J. &Filipe, M. I. (1978) Variations in sialo mucins in the mucosa of large intestine in malignancy: a quantimet and statistical analysis.Histochem. J. 10, 559–72.
Faillard, H. &Schauer, R. (1972) Glycoproteins as lubricants, protective agents, carriers, structural proteins and as participants in other functions. InGlycoproteins: Their Composition, Structure and Function (edited byGottschalk, A.), Vol. 5, pp. 1256–67. Amsterdam, London, New York: Elsevier.
Fenger, C. &Filipe, M. I. (1977) Pathology of the anal glands with special reference to their mucin histochemistry.Acta path. microbiol. scand. 85, 273–85.
Filipe, M. I. (1979) Mucins in the human gastrointestinal epithelium: a review.Invest. Cell Path. 2, 195–216.
Filipe, M. I. &Cooke, K. B. (1974), Changes in the composition of mucin in the mucosa adjacent to carcinoma of the colon as compared with normal: a biochemical investigation.J. clin. Path. 27, 315–8.
Filipe, M. I. &Fenger, C. (1979) Histochemical characteristics of mucins in the small intestine. A comparative study of normal mucosa, benign epithelial tumours and carcinoma.Histochem. J. 11, 277–87.
Florey, H. (1955) Mucin and protection in the body.Proc. R. Soc. Lond. Biol. Series B. 143, 147–59.
Forstner, J. F. (1978) Intestinal mucin in health and disease.Digestion 17, 234–63.
Fraser, G. M. &Clamp, J. R. (1975) Changes in human colonic mucus in ulcerative colitis.Gut 16, 832–3.
Gold, D. V. &Miller, F. (1974) Characterisation of human mucoprotein antigen.Immunochemistry 11, 369–75.
Gold, D. V. &Miller, F. (1975) Chemical and immunological differences between normal and tumoral colonic mucoprotein antigen.Nature, Lond. 255 85–7.
Gold, D. V. &Miller, F. (1978) Composition of human colonic mucoprotein antigen from normal and neoplastic mucosa.Cancer Res. 38 3204–11.
Gold, D. V., Shochat, D. &Miller, F. (1981) Protease digestion of colonic mucin. Evidence of the existence of two immunochemically distinct mucins.J. biol. Chem. 256 6354–8.
Kent, P. W. (1962) The chemistry of mucoprotein: an introduction to gastrointestinal mucus.Gastroenterology 43, 292–303.
Kim, Y. S. &Isaacs, R. (1975) Glycoprotein metabolism in inflammatory and neoplastic disease of the human colon.Cancer Res. 35, 2092–7.
Korhonen, L. K. &Makela, V. (1971) Carbohydrate-rich compounds in the colonic mucosa of man III. A preliminary report of some biochemical characteristics of the carbohydrate-rich components in normal colonic mucosa.Histochem. J. 3, 101–6.
Lehnhardt, W. F. &Winzler, R. J. (1968) Determination of neutral sugars in glycoproteins by gas-liquid chromatography.J. Chromatogr. 34, 471–9.
Ludowieg, J. J. &Dorfman, A. (1960) A micromethod for the colorimetric determination ofN-acetyl groups in acid mucopolysaccharides.Biochim. biophys. Acta. 38, 212–8.
Morson, B. C. (1975) Pathology of ulcerative colitis. InInflammatory Bowel Disease (edited byKirsner, J. B. andShorter, R. G.), pp 167–81 Philadelphia: Lea and Febiger.
Ramey, C. W., Culling, C. F. A., Reid, P. E., Dunn, W. L. &Clay, M. G. (1979) Simple inexpensive apparatus for the rapid DEAE-cellulose chromatography of small quantities of glycoproteins.J. Chromatogr. (Biomedical Applications) 164, 382–5.
Reid, P. E., Culling, C. F. A. &Dunn, W. L. (1973) Saponification induced increase in the periodic acid-Schiff reaction in the gastrointestinal tract mechanism and distribution of the reactive substance.J. Histochem. cytochem. 21, 473–82.
Reid, P. E., Culling, C. F. A. &Dunn, W. L. (1978b) A histochemical method for the demonstration of 9-O-acyl sialic acids. An investigation of bovine submaxillary mucin and intestinal mucins.J. Histochem. Cytochem. 26, 187–92.
Reid, P. E., Culling, C. F. A., Dunn, W. L. &Clay, M. G. (1976) The use of a transesterification technique to distinguish between certain neuraminidase resistant epithelial glycoprotein.Can. J. Biochem. 55, 493–503.
Reid, P. E., Culling, C. F. A., Dunn, W. L., Clay, M. G. &Ramey, C. W. (1978a) A correlative chemical and histochemical study of theO-acetylated sialic acids of human colonic epithelial glycoproteins in formalin fixed paraffin embedded tissues.J. Histochem. Cytochem. 26, 1033–41.
Reid, P. E., Culling, C. F. A., Dunn, W. L., Ramey, C. W. &Clay, M. G. (1975b) Differences in chemical composition between the epithelial glycoproteins of the upper and lower halves of rat colon.Can. J. Biochem. 53, 1328–32.
Reid, P. E., Culling, C. F. A., Ramey, C. W., Dunn, W. L. &Clay, M. G. (1977) A simple method for the determination of theO-acetyl substitution pattern of the sialic acids of colonic epithelial glycoprotein.Can. J. Biochem. 55, 493–503.
Reid, P. E., Culling, C. F. A., Ramey, C. W., Dunn, W. L. Magil, A. B. &Clay, M. G. (1980) Differences between theO-acetylated sialic acids of the epithelial mucins of human colonic tumours and normal controls: a correlative chemical and histochemical study.J. Histochem. Cytochem. 28, 217–22.
Reid, P. E., Culling, C. F. A., Tsang, Wai-Chui, Ramey, C. W., Dunn, W. L. &Clay, M. G. (1975a) The demonstration ofO-acetylated sialic acids in colonic epithelial glycoproteins.Can. J. Biochem. 53, 388–91.
Roelfs, R. E., Gibbs, G. E. &Griffin, G. D. (1967) The composition of rectal mucus in cystic fibrosis.Am. J. Dis. Child. 113, 419–21.
Rogers, C. M., Cooke, K. B. &Filipe, M. I. (1978) Sialic acids of human large bowel mucosa:O-acetylated variants in normal and malignant states.Gut 19, 587–92.
Soergel, K. H. &Ingelfinger, F. J. (1961) Proteins in serum and rectal mucus of patients with ulcerative colitis.Gastroenterology 40, 37–46.
Soergel, K. H. &Ingelfinger, F. J. (1964) Composition of rectal mucus in normal subjects and patients with ulcerative colitis.Gastroenterology 47, 610–16.
Spicer, S. S. (1965) Diamine methods for differentiating mucosubstances histochemically.J. Histochem. Cytochem. 13, 211–34.
Sky-Peck, H. H., Lundgren, R. &Borstein, I. (1966) Acid mucopolysaccharide composition in human colon.Ann. N.Y. Acad. Sci. 130, 951–962.
Teague, R. H., Fraser, D. &Clamp, J. R. (1973) Changes in monosaccharide content of mucous glycoproteins in ulcerative colitis.Br. Med. J. 2, 645–6.
Terho, T. &Laitio, M. (1977) Hexosamine containing macromolecules in human colon carcinomas.Scand. J. Gastroenterol. 12, 7–15.
Whitehead, R. (1979) Ulcerative colitis, Crohn's colitis, ischaemic colitis and pseudomembranous colitis. InMucosal Biopsy of the Gastrointestinal Tract., 2nd edn, Vol. 3, pp. 152–95. Philadelphia, Toronto, London: Saunders.
Winzler, R. J. (1955) Determination of serum glycoproteins.Meth. biochem Analysis 2, 279–311.
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Reid, P.E., Culling, C.F.A., Dunn, W.L. et al. Chemical and histochemical studies of normal and diseased human gastrointestinal tract. I. A comparison between histologically normal colon, colonic tumours, ulcerative colitis and diverticular disease of the colon. Histochem J 16, 235–251 (1984). https://doi.org/10.1007/BF01003608
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DOI: https://doi.org/10.1007/BF01003608