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Enhancement of enzyme activity in aqueous-organic solvent mixtures

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Summary

The activities of poyphenol oxidase, peroxidase, acid phosphatase and trypsin were measured in the presence of four different water miscible solvents, acetonitrile; N,N dimethylformamide; tetrahydrofuran and dioxane. Stimulation of enzymatic activities was observed in all the cases at a specific concentration range of cosolvent added which cannot be correlated either with log P or denaturation capacity. Also, no significant structural changes were observed by fluorescence studies even at the cosolvent concentrations where activity increases were significant.

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Authors and Affiliations

  1. Chemistry Department, II T Delhi, 110016, India

    Renu Batra & Munishwar N. Gupta

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  1. Renu Batra
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  2. Munishwar N. Gupta
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Batra, R., Gupta, M.N. Enhancement of enzyme activity in aqueous-organic solvent mixtures. Biotechnol Lett 16, 1059–1064 (1994). https://doi.org/10.1007/BF01022403

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