Summary
Explants from petioles, folioles or hypocotyls ofOnonis natrix have been used for calli initiation. Hypocotyls inoculated on MS medium supplemented with 2% sucrose and 0.5 mg.1−1 2,4-D / 1 mg.1−1 Kin showed to be the best primary explant. Cell suspension cultures were established in MS basal medium supplemented with 2% sucrose, 0.5 mg.1−1 NAA or 2,4-D and 1 mg.1−1 Kin. Different subculturing periods, inoculum density, hormonal supplementation and sucrose concentration were assayed in order to obtain the best culture growth conditions. The optimal conditions were achieved with cultures initiated with 40 g.1−1 of initial inoculum, growing in MS basal medium supplemented with 4% sucrose, 0.5 mg.1−1 NAA and 1 mg.1−1 Kin subcultured every twelve days. Under these experimental conditions, the cultures showed a doubling time of 36.3 hours.
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References
Aitchison, P. A.; A. J. Macleod; M. M. Yeoman, 1977. In “Plant Tissue and Cell Culture” (ed. H. E. Street). Blackwell Scientific Publications, Oxford.
Amorim, H. V.; D. K. Dougall; W. R. Sharp, 1977. Physiol. Plant. 39: 91–95.
De-Eknamkul, W.; B. E. Ellis, 1985. Plant Cell Reports 4: 50–53.
Do, C. B.; F. Cornier, 1991. Plant Cell Reports 9: 500–504.
Drapeau, D.; H. Blanch; C. Wilke, 1986. Biotechnol. Bioengng. 28: 1555–1563.
Feliciano, A. S.; A. F. Barrero; M. Madarde; J. M. M. Del Corral; M. V. Calle, 1983. Phytochemistry 22: 2031–2033.
Figueiredo, A. C.; M. S. Pais, 1991. Biotechnol. Lett. 13: 63–68.
Figueiredo, A. C.; P. Fevereiro; J. Cabral; J. Novais; M. S. Pais 1987. Biotechnol. Lett. 9: 213–218.
Font Quer, P. V., 1981. “Plantas Medicinales. El Dioscorides Renovado” (ed. Labor, S. A.).
Gamborg, O.; R. Miller; K. Ojima, 1968. Exp. Cell Res. 50: 151–158.
Larkin, P., 1976. Planta 128: 213–216.
Murashige, T.; F. Skoog, 1962. Physiol. Plant. 15: 473–497.
Phillips, R.; G. Henshaw, 1977. J. Exp. Bot. 28: 785–794.
Schripsema, J.; A. Meijer; F. Van Iren; H. Hoopen; R. Verpoorte, 1990. Plant Cell, Tissue and Organ Culture 22: 55–64.
Schulz, A., 1981. PH. D. Thesis Technische Universitat. Hannover.
Street, H. E., 1977. In “Plant Tissue and Cell Culture” (ed. H. E. Street). Blackwell Scientific Publications, Oxford.
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Graça Miguel, M., Barroso, J.G. Cell suspension cultures ofOnonis natrix L. subspeciesramosissima: Establishment and culture conditions. Biotechnol Lett 14, 223–226 (1992). https://doi.org/10.1007/BF01023363
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DOI: https://doi.org/10.1007/BF01023363