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Development of semi-continuous and continuous flow bioreactors for the high level production of chloroperoxidase

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Summary

Continuous and semi-continuous flow bioreactors have been developed for the production of chloroperoxidase, which is secreted by the fungusCaldariomyces fumago. The semi-continuous system facilitated the harvest of mycelium-free supernatant for 200 days from a single inoculum, with enzyme levels greater than 600 mg/l during the first 80 days. The continuous flow bioreactor yielded essentially pure enzyme at the rate of 117 mg/day.

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Authors and Affiliations

  1. 403 Roger Adams Laboratory, Department of Biochemistry, University of Illinois, 61801, Urbana, IL

    Steven R. Blanke, San Yi & Lowell P. Hager

Authors
  1. Steven R. Blanke
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  2. San Yi
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  3. Lowell P. Hager
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Blanke, S.R., Yi, S. & Hager, L.P. Development of semi-continuous and continuous flow bioreactors for the high level production of chloroperoxidase. Biotechnol Lett 11, 769–774 (1989). https://doi.org/10.1007/BF01026094

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  • DOI: https://doi.org/10.1007/BF01026094

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