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Hydrophobic interaction chromatography of penicillin amidase

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Summary

For hydrophobic binding of penicillin amidase to modified Sepharose, a phenyl group or a hydrophobic aliphatic moiety (leucyl, octyl) is necessary. Concentration and purification of the enzyme can then be achieved in a single step.

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Authors and Affiliations

  1. Research and Development, Hindustan Antibiotics Ltd., Pimpri, 411018, Pune, India

    Vayalombron K. Sudhakaran & Jaiprakash G. Shewale

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  1. Vayalombron K. Sudhakaran
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  2. Jaiprakash G. Shewale
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Sudhakaran, V.K., Shewale, J.G. Hydrophobic interaction chromatography of penicillin amidase. Biotechnol Lett 9, 539–542 (1987). https://doi.org/10.1007/BF01026657

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  • DOI: https://doi.org/10.1007/BF01026657

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