Abstract
A general method is described for the assay of glycosyltransferase activity, which makes use of synthetic glycoside acceptors attached to hydrophobic aglycones. The products formed by incubation of an enzyme with acceptor and radiolabelled sugarnucleotide can then be rapidly (one minute) separated from interfering radioactivity by adsorption on to reverse-phase C-18 cartridges. After aqueous washing, products are easily isolated by elution with methanol. The utility of the method for the assay of β(1–4)galactosyltransferase, α(1–2)fucosyltransferase andN-acetylglucosaminyltransferase I and V is demonstrated.
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Palcic, M.M., Heerze, L.D., Pierce, M. et al. The use of hydrophobic synthetic glycosides as acceptors in glycosyltransferase assays. Glycoconjugate J 5, 49–63 (1988). https://doi.org/10.1007/BF01048331
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DOI: https://doi.org/10.1007/BF01048331