Skip to main content
SpringerLink
Log in

Simultaneous expression by porcine aorta endothelial cells of glycosphingolipids bearing the major epitope for human xenoreactive antibodies (Galα1–3Gal), blood group H determinant andN-glycolylneuraminic acid

  • Papers
  • Published:
Glycoconjugate Journal Aims and scope Submit manuscript

Abstract

Glycosphingolipids were isolated from primary cultures of porcine endothelial cells labelled with14C-galactose or14C-glucosamine. They were characterized by their mobility on thin layer chromatogram, their sensitivity to exoglycosidases, and their labelling with antibodies. In addition to the major glycosphingolipids, globotetra-and globotriaosylceramide, minor ones were identified as penta-and heptaglycosylceramide of the neolactoseries terminated by either Galα1–3Gal-(xenoreactive epitope) or Fucα1–2Gal-(H determinant). Two gangliosides were found, GM3 and GD3, andN-glycolylneuraminic acid was their major sialic acid. Therefore, porcine endothelial cells differ from human endothelial cells by expression of glycosphingolipids that are absent in man: two Galα1–3Gal-terminated glycolipids recognized by human natural antibodies, and twoN-glycolylneuraminic acid-terminated gangliosides which are potent immunogens.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

HPTLC:

high performance thin-layer chromatography

GSL:

glycosphingolipid

NeuAc:

N-acetylneuraminic acid

NeuGc:

N-glycolylneuraminic acid

PAEC:

porcine aorta endothelial cell

References

  1. Good AH, Cooper DKC, Malcolm AJ, Ippolito RM, Koren E, Neethling FA, Ye Y, Zuhdi N, Lamontagne LR (1992)Transplant Proc 24: 559–62.

    PubMed  Google Scholar 

  2. Galili U (1993)Immunology Today 14: 480–82.

    PubMed  Google Scholar 

  3. Collins BH, Parker W, Platt JL (1994)Xenotransplantation 1: 36–46.

    Google Scholar 

  4. Galili U, Shohet SB, Kobrin E, Stults CLM, Macher BA (1988).J Biol Chem 263: 17755–62.

    PubMed  Google Scholar 

  5. Oriol R, Ye Y, Koren E, Cooper DKC (1993)Transplantation 56: 1433–42.

    PubMed  Google Scholar 

  6. Oriol R, Mollicone R, Coullin P, Dalix AM, Candelier JJ (1992)APMIS 100: 28–38.

    Google Scholar 

  7. Cooper DKC, Koren E, Oriol R (1994)Xeno 2: 22–25.

    Google Scholar 

  8. Sandrin MS, Fodor WL, Mouhtouris W, Osman N, Cohney S, Rollins SA, Guilmette ER, Setter E, Squinto SP, McKenzie IFC (1995)Nature Med 1: 1261–67.

    PubMed  Google Scholar 

  9. Higashi H, Naiki M, Matuo S, Okouchi K (1977)Biochem Biophys Res Commun 79: 388–95.

    PubMed  Google Scholar 

  10. Merrick JM, Zadarlik K, Milgrom F (1978)Int Arch Allergy Appl Immun 7: 477–80.

    Google Scholar 

  11. Bouhours D, Bouhours JF (1994)Xeno 2: 33–34.

    Google Scholar 

  12. Bouhours D, Bouhours JF (1988)J Biol Chem 263: 15540–45.

    PubMed  Google Scholar 

  13. Saito T, Hakomori S (1971)J Lipid Res 12: 257–59.

    PubMed  Google Scholar 

  14. Ueno K, Ando S, Yu RK (1978)J Lipid Res 19: 863–71.

    PubMed  Google Scholar 

  15. Magnani JL, Smith DF, Ginsburg V (1980)Anal Biochem 109: 399–402.

    PubMed  Google Scholar 

  16. Hansson GC, Karlsson KA, Larson G, Samuelsson B, Thurin J, Bjursten LM (1985)J Immunol Method 83: 37–42.

    PubMed  Google Scholar 

  17. Piller F, Piller V (1993) InGlycobiology, A Practical Approach (Fukuda M, Kobata A, eds) pp. 291–328. Oxford: University Press.

    Google Scholar 

  18. Watanabe K, Laine RA, Hakomori S (1975)Biochemistry 14: 2725–33.

    PubMed  Google Scholar 

  19. Egge H, Kordowicz M, Peter-Katalinic J, Hanfland P (1985)J Biol Chem 260: 4927–35.

    PubMed  Google Scholar 

  20. Samuelsson BE, Rydberg L, Breimer ME, Bäcker A, Gustavsson M, Holgersson J, Karlsson E, Uyterwaal AC, Cairn T, Welsh K (1994)Immunol Rev 141: 151–68.

    PubMed  Google Scholar 

  21. Gillard BK, Jones MA, Marcus DM (1987)Arch Biochem Biophys 256: 435–45.

    PubMed  Google Scholar 

  22. Larsen RD, Rivera-Marrero CA, Ernst LK, Cummings RD, Lowe JB (1990)J Biol Chem 265: 7055–61.

    PubMed  Google Scholar 

  23. Joziasse DH, Shaper JH, Jabs EW, Shaper NL (1991)J Biol Chem 266: 6991–98.

    PubMed  Google Scholar 

  24. Galili U, Anaraki F, Thall A, Hill-Black C, Radic M (1993)Blood 82: 2485–93.

    PubMed  Google Scholar 

  25. Jalali-Araghi K, Macher BA (1994)Glycoconjugate J 11: 266–71.

    Google Scholar 

  26. Chien JL, Li SC, Li YT (1979)J Lipid Res 20: 669–73.

    PubMed  Google Scholar 

  27. Blanken WM, Van den Eijnden DH (1985)J Biol Chem 260: 12927–34.

    PubMed  Google Scholar 

  28. Watkins WM (1980)Adv. Hum. Genet. 10: 1–136.

    PubMed  Google Scholar 

  29. Platt JL, Lindman BJ, Chen H, Spitalnik SL, Bach F (1990)Transplantation 50: 817–22.

    PubMed  Google Scholar 

  30. Suzuki Y, Suzuki N, Michi H, Matsumoto M (1985)Lipids 9: 588–93.

    Google Scholar 

  31. Kawano T, Kozutsumi Y, Kawasaki T, Suzuki A (1994)J Biol Chem 269: 9024–29.

    PubMed  Google Scholar 

  32. Hamanaka S, Handa S, Inoue J, Hasegawa A, Yamakawa T (1979)J Biochem. (Tokyo) 86: 695–98.

    Google Scholar 

  33. Hashimoto Y, Yamakawa T, Tanabe Y (1984)J Biochem (Tokyo) 96: 1777–82.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut de Transplantation et de Recherche en Transplantation, INSERM U. 437, Centre Hospitalier Régional, F-44035, Nantes Cedex, France

    Daniele Bouhours, Christine Pourcel & Jean-Francois Bouhours

Authors
  1. Daniele Bouhours
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christine Pourcel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jean-Francois Bouhours
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bouhours, D., Pourcel, C. & Bouhours, JF. Simultaneous expression by porcine aorta endothelial cells of glycosphingolipids bearing the major epitope for human xenoreactive antibodies (Galα1–3Gal), blood group H determinant andN-glycolylneuraminic acid. Glycoconjugate J 13, 947–953 (1996). https://doi.org/10.1007/BF01053190

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01053190

Keywords

Search

Navigation